Patents by Inventor Kien Truc Giang Nguyen
Kien Truc Giang Nguyen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11795488Abstract: The present invention relates to a method of ligating a first peptide via its C-terminus to the N-terminus of a second peptide, wherein the reaction is catalyzed by an asparagine/aspartate (Asx) peptide ligase OaAEPI Cys247Ala having the amino acid sequence of SEQ ID NO: 1. Further encompassed are a method of preparing a dimer, oligomer, or multimer of one or more peptides of interest and a method of modifying or tagging the surface of a target cell by one or more peptides of interest. Also encompassed in the invention are the ligated peptides and/or tagged target cells obtainable according to any of the methods, the peptide ligase OaAEPI Cys247Ala having the amino acid sequence of SEQ ID NO: 1, as well as kits comprising said peptide ligase.Type: GrantFiled: September 12, 2017Date of Patent: October 24, 2023Assignee: NANYANG TECHNOLOGICAL UNIVERSITYInventors: Bin Wu, Renliang Yang, Yee Hwa Wong, Julien Lescar, Chuan Fa Liu, James P Tam, Kien Truc Giang Nguyen, Ziqi Long
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Patent number: 11788105Abstract: Described are methods of using a lipase for hydrolysis and esterification. In a first method of producing a medium chain fatty acid by hydrolysis, the method comprises providing a polypeptide with at least 90% degree of identity to SEQ ID No. 3, and contacting the polypeptide with a medium chain fatty acid ester and water to produce the medium chain fatty acid. In a second method of forming an ester, the method comprises providing a polypeptide with at least 90% degree of identity to SEQ ID No. 3; and contacting the polypeptide with a long chain fatty acid, an alcohol, and water to form the ester of the long chain fatty acid and the alcohol.Type: GrantFiled: December 30, 2020Date of Patent: October 17, 2023Assignee: Wilmar International LimitedInventors: Ren Liang Yang, Hong Fang Zhang, Weijian Ye, Mong Jie Andre Ng, Kien Truc Giang Nguyen
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Publication number: 20230128192Abstract: The present invention relates to a method of ligating a first peptide via its C-terminus to the N-terminus of a second peptide, wherein the reaction is catalyzed by an asparagine/aspartate (Asx) peptide ligase OaAEPI Cys247Ala having the amino acid sequence of SEQ ID NO: 1. Further encompassed are a method of preparing a dimer, oligomer, or multimer of one or more peptides of interest and a method of modifying or tagging the surface of a target cell by one or more peptides of interest. Also encompassed in the invention are the ligated peptides and/or tagged target cells obtainable according to any of the methods, the peptide ligase OaAEPI Cys247Ala having the amino acid sequence of SEQ ID NO: 1, as well as kits comprising said peptide ligase.Type: ApplicationFiled: September 30, 2022Publication date: April 27, 2023Inventors: Bin WU, Renliang YANG, Yee Hwa WONG, Julien LESCAR, Chuan Fa LIU, James P TAM, Kien Truc Giang NGUYEN, Ziqi LONG
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Publication number: 20220010341Abstract: The present invention is in the field of oil processing, and specifically the present invention relates to a process of hydrolyzing an oil with high melting point, such as hydrogenated castor oil (HCO), by lipase. In an embodiment of the invention, there is provided a process of hydrolyzing an oil having a higher melting point to obtain a hydrolysis product, the process comprising: a step of mixing water, an organic solvent, and a lipase with the oil to form a mixture, and a step of hydrolyzing the oil in the mixture, wherein the lipase comprises one or more lipase derived from Thermomyces sp. but not any monoacylglycerol lipase or any lipase derived from Rhizomucor sp., and the organic solvent is any one selected from a sterically hindered alcohol, a non-polar organic solvent, and a mixture of a water-miscible organic solvent with a non-polar organic solvent.Type: ApplicationFiled: October 2, 2019Publication date: January 13, 2022Applicant: WILMAR INTERNATIONAL LIMITEDInventors: Kien Truc Giang NGUYEN, Ren Liang YANG
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Patent number: 11124788Abstract: The present invention is directed to enzymes having Asx-specific ligase and cyclase activity and to nucleic acids encoding those as well as methods of the manufacture of said enzymes. Further encompassed are methods and uses of these enzymes.Type: GrantFiled: February 10, 2020Date of Patent: September 21, 2021Assignee: NANYANG TECHNOLOGICAL UNIVERSITYInventors: Kien Truc Giang Nguyen, James P. Tam
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Patent number: 11091786Abstract: The present invention relates to a method of forming a peptide of Formula (I) (P1-Xaa1-Xaa2-P2) by ligating a first peptide of Formula (II) (P1-Xaa1-X—R, wherein X is O or S) to a second peptide of Formula (III) (Xaa1-Xaa2-P2) by enzymatically cleaving the bond between “Asx” and “X” in the first peptide of Formula (II) and ligating the fragment P1-Asx of the first peptide to the second peptide of Formula (III), wherein the enzymatic cleavage and ligation reaction is catalyzed by butelase 1 (SEQ ID NO: 1) and the peptide of Formula (I) is a depsipeptide, preferably a thiodepsipeptide. Further encompassed are peptides and dendrimeric peptide assemblies prepared using the presently disclosed method, as well as use of the dendrimeric peptide assemblies as a vaccine, medicament, or diagnostic agent, particularly as an antimicrobial agent.Type: GrantFiled: September 29, 2016Date of Patent: August 17, 2021Assignee: Nanyang Technological UniversityInventors: James P Tam, Kien Truc Giang Nguyen, Yuan Cao, Chuan Fa Liu
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Publication number: 20210198704Abstract: Described are methods of using a lipase for hydrolysis and esterification. In a first method of producing a medium chain fatty acid by hydrolysis, the method comprises providing a polypeptide with at least 90% degree of identity to SEQ ID NO. 3, and contacting the polypeptide with a medium chain fatty acid ester and water to produce the medium chain fatty acid. In a second method of forming an ester, the method comprises providing a polypeptide with at least 90% degree of identity to SEQ ID NO. 3; and contacting the polypeptide with a long chain fatty acid, an alcohol, and water to form the ester of the long chain fatty acid and the alcohol.Type: ApplicationFiled: December 30, 2020Publication date: July 1, 2021Applicant: Wilmar International LimitedInventors: Ren Liang YANG, Hong Fang ZHANG, Weijian YE, Mong Jie Andre NG, Kien Truc Giang NGUYEN
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Publication number: 20200172893Abstract: The present invention is directed to enzymes having Asx-specific ligase and cyclase activity and to nucleic acids encoding those as well as methods of the manufacture of said enzymes. Further encompassed are methods and uses of these enzymes.Type: ApplicationFiled: February 10, 2020Publication date: June 4, 2020Inventors: Kien Truc Giang Nguyen, James P. Tam
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Patent number: 10590407Abstract: The present invention is directed to enzymes having Asx-specific ligase and cyclase activity and to nucleic acids encoding those as well as methods of the manufacture of said enzymes. Further encompassed are methods and uses of these enzymes.Type: GrantFiled: March 24, 2015Date of Patent: March 17, 2020Assignee: Nanyang Technological UniversityInventors: Kien Truc Giang Nguyen, James P. Tam
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Publication number: 20190218586Abstract: The present invention relates to a method of ligating a first peptide via its C-terminus to the N-terminus of a second peptide, wherein the reaction is catalyzed by an asparagine/aspartate (Asx) peptide ligase OaAEPI Cys247Ala having the amino acid sequence of SEQ ID NO: 1. Further encompassed are a method of preparing a dimer, oligomer, or multimer of one or more peptides of interest and a method of modifying or tagging the surface of a target cell by one or more peptides of interest. Also encompassed in the invention are the ligated peptides and/or tagged target cells obtainable according to any of the methods, the peptide ligase OaAEPI Cys247Ala having the amino acid sequence of SEQ ID NO: 1, as well as kits comprising said peptide ligase.Type: ApplicationFiled: September 12, 2017Publication date: July 18, 2019Inventors: Bin WU, Renliang YANG, Yee Hwa WONG, Julien LESCAR, Chuan Fa LIU, James P TAM, Kien Truc Giang NGUYEN, Ziqi LONG
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Publication number: 20180274003Abstract: The present invention relates to a method of forming a peptide of Formula (I) (P1-Xaa1-Xaa2-P2) by ligating a first peptide of Formula (II) (P1-Xaa1-X—R, wherein X is O or S) to a second peptide of Formula (II I) (Xaa1-Xaa2-P2) by enzymatically cleaving the bond between “Asx” and “X” in the first peptide of Formula (II) and ligating the fragment P1-Asx of the first peptide to the second peptide of Formula (III), wherein the enzymatic cleavage and ligation reaction is catalyzed by butelase 1 (SEQ ID NO: 1) and the peptide of Formula (I) is a depsipeptide, preferably a thiodepsipeptide. Further encompassed are peptides and dendrimeric peptide assemblies prepared using the presently disclosed method, as well as use of the dendrimeric peptide assemblies as a vaccine, medicament, or diagnostic agent, particularly as an antimicrobial agent.Type: ApplicationFiled: September 29, 2016Publication date: September 27, 2018Inventors: James P Tam, Kien Truc Giang Nguyen, Yuan Cao, Chuan Fa Liu
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Publication number: 20170044515Abstract: The present invention is directed to enzymes having Asx-specific ligase and cyclase activity and to nucleic acids encoding those as well as methods of the manufacture of said enzymes. Further encompassed are methods and uses of these enzymes.Type: ApplicationFiled: March 24, 2015Publication date: February 16, 2017Inventors: Kien Truc Giang Nguyen, James P. Tam