Abstract: A first method of purifying apolipoprotein A-1 includes mixing plasma fraction IV with a 1-8 M urea solution to form a pretreatment solution; loading the pretreatment solution to a first anion chromatography column, and then eluting to obtain an apoA-1 protein solution; and loading the apoA-1 protein solution to a second anion chromatography column, and eluting to obtain pure apoA-1 protein. A second method includes dissolving plasma fraction IV in a buffer to produce a pretreatment solution; adding NaCl to the pretreatment solution and cooling it to form apoA-1 precipitate; collecting and reconstituting the apoA-1 precipitate; loading the reconstituted apoA-1 to an anion exchange column; and eluting apoA-1 from the column.

Abstract: A kit of lyophilized thrombin and lyophilized fibrinogen comprises fibrinogen of 50-100 mg/ml, thrombin of 100-1000 IU/ml and 20-60 mmol/L CaCl2 used to compound fibrin membrane. The lyophilized thrombin and lyophilized fibrinogen are applied in a clinical tumor operation for reducing the risk of tumor metastasis after the operation. The solutions of thrombin and fibrinogen are daubed on the surface of a tumor, where they mix and form a solid-meshy fibrin membrane that prevents pervasion of tumor cells caused by incision and trauma in the tumor operation, thereby reducing the risk of palindromia and metabasis after the operation and improving the patient's life span.