Abstract: A novel polypeptide which is at least equivalent in biological activity to IFN-.gamma. and is resistant to dimerization and polymerization, a transformant which carries a DNA coding for a novel polypeptide, and a method of producing a novel polypeptide from the culture of the transformant.The DNA coding for the novel polypeptide can be produced, for example, starting with a known plasmid which contains the IFN-.gamma. gene (cDNA), namely pHITtrp1101 or pHITtrp 1201.Insertion of this DNA into a vector followed by introduction into a host gives the transformant. An antibody column is used for the purification of the polypeptide from the culture.The novel polypeptide produced can be used as an antiviral agent or an antitumor agent.

Abstract: A DNA having a 5'-inosinate dehydrogenase gene and further having a Hind III cleavage site 2.9 kilo base pairs can be produced from the chromosomal DNA of a guanosine and/or xanthosine-producing strain of the genus Bacillus. A vector with the DNA obtained above is used to transform Bacillus strain capable of producing guanosine, and the transformed Bacillus strain is useful to increase the guanosine productivity as compared with the case in which a strain before transformantion is used.

Abstract: A novel recombinant DNA containing the base sequence shown in FIG. 1 or a portion thereof which exhibits promoter activity. The base sequence exhibiting a potent promoter activity is obtained from the chromosomal DNA of strain Bacillus by, for example, cloning with a cloning vector. By growing a transformant of Bacillus transformed with the recombinant DNA carrying a peptide encoding nucleotide, the desired peptide may be produced.