Abstract: A method is provided for the regeneration of an individual coconut plantlet. It comprises culturing a tissue explant of an undifferentiated male flower of a coconut tree in a culture medium, which contains 10-50 ppm of an auxin, 0.1-3% of a polysaccharide produced by a bacterium of Pseudomonas and 0.05-1% of activated carbon, in a dark place until a shoot-like structure appears; and then culturing under exposure to light of 3,000-20,000 luxes while repeating its subculture in a fresh culture medium.

Abstract: A composition for curing autoimmune diseases is disclosed. The composition comprises as an effective component an acidic heteropolysaccharide which is secreted by a callus induced from a plant belonging to the genus Polianthes L. The acidic heteropolysaccharide can be prepared as a uniform product by the application of plant tissue culture methods and exhibits superior capability of removing immunocomplexes with the least side effects.

Abstract: A novel acidic heteropolysaccharide which comprises as its structural units arabinose, mannose, galactose, glucuronic acid, and xylose, at a specific ratio. The polysaccharide can be prepared by culturing a callus induced from a plant belonging to the genus Polianthes L. in a culture medium containing plant hormones. The polysaccharide can be used as a component of agents for external application and detergents, and provides good moisturizing effects and a smooth, satisfying feeling upon use. When formulated in detergents, they produce remarkable improvement in a clean, rich feeling upon use.

Abstract: A novel detergent composition for clothing comprises a cellulase having a non-degrading index of the following equation of not less than 500 ##EQU1## The composition can not only remove the dirt in clothing articles without damaging them but also show the effect of mitigating the roughness of cloth to the touch and the effect of preventing decoloration.

Abstract: Alkaline cellulases having an optimum pH in an alkaline range and being stable over a broad pH range are produced by microorganisms which belong to the genus Bacillus and grow in a neutral medium.These alkaline cellulases are rarely inhibited by ingredients ordinarily incorporated in detergents such as surface active agents, proteinases and chelating agents, so that they can be conveniently used in detergent compositions.

Abstract: Novel alkaline cellulase K, CMCase I and CMCase II are obtained by isolation from a culture product of Bacillus sp KSM-635.These enzymes stably work in a wide range including an alkaline side, and their activity is shown even at low temperatures. Further, they have a strong resistance to surface active agents, chelating agents and proteinases. Therefore, they can be effectively utilized not only as an additive for clothing detergents, but also as a biomass and in other fields.

Abstract: Novel alkali-resistant cellulases which have an optimum pH at a neutral region and have a high relative activity in an alkaline region are produced by microorganisms belonging to the genus Bacillus and growing in neutral medium.The cellulases suffer little inhibition by means of detergent ingredients such as surface active agents, proteinases and chelating agents. Therefore, they can be conveniently used as one of ingredients for detergent compositions.

Abstract: A novel acidic heteropolysaccharide which comprises as its structural units arabinose, mannose, galactose, glucuronic acid, and xylose, at a specific ratio. The polysaccharide can be prepared by culturing a callus induced from a plant belonging to the genus Polianthes L. in a culture medium containing plant hormones. The polysaccharide can be used as a component of agents for external application and detergents, and provides good moisturizing effects and a smooth, satisfying feeling upon use. When formulated in detergents, they produce remarkable improvement in a clean, rich feeling upon use.

Abstract: DNA frangments containing an alkaline cellulase K gene, which comprise about 4.0, about 2.4 and about 1.9 kilo base pairs, respectively, each having a specific restruction map, recombinant plasmids containing any of these DNA fragments, and recombinant microorganisms harboring any of said plasmids.The alkaline cellulase K gene is derived from a bacterial strain which belongs to the genus Bacillus and is alkalophilic, that is, capable of revealing an optimum growth in an alkaline pH region.

Abstract: A novel detergent composition for clothing comprises a cellulase having a non-degrading index of the following equation of not less than 500 ##EQU1## The composition can not only remove the dirt in clothing articles without damaging them but also show the effect of mitigating the roughness of cloth to the touch and the effect of preventing decoloration.

Abstract: A branched fatty acid cholesterol ester represented by the formula (I): ##STR1## where, R is a saturated aliphatic hydrocarbon group having a total of 11 to 23 carbon atoms and including at least one alkyl substituent group attached on the main chain inbetween the carboxyl-bonding position and the center of the main chain; and cosmetic compositions containing the same are disclosed.

Abstract: A non-allergenic lanolin or a derivative thereof which does not contain any substances which have an Rf value of less than 0.38 with a chloroform solvent or less than 0.14 with a benzene solvent, in thin layer chromatography using magnesium silicate having a thickness of 0.25 mm as the adsorbent.

Abstract: A process is provided for producing a lower allergenic lanolin or derivative thereof by liquid-liquid extracting lanolin or its derivative with a non-polar hydrocarbon solvent and a mixed solvent of water with a lower alcohol. The non-polar hydrocarbon solvent extract is then recovered.