Abstract: Disclosed herein are methods of using serum nitric oxide levels to diagnose subjects as having or likely to develop a post-operative complication.

Abstract: Disclosed herein are methods of using serum nitric oxide levels to diagnose subjects as having or likely to develop a post-operative complication.

Abstract: An article tether is provided. The article tether may include a tethering cord, a first coupling, a second coupling, an anchor and a clasp. The tethering cord may include a first end and a second end. The first coupling and the second coupling may form tubular openings sized for slidably receiving the tethering cord but not the anchor. The user may thread the first end through the second coupling opening and then the first coupling opening sequentially in a specified direction. Then the user may connect the first end to the anchor. Then the user may thread the second end through the first coupling opening in the same specified direction so that the tethering cord defines a loop opening. The user may then fit the loop opening around an article or portion thereof. The user may then adjust the size of the loop opening so as to securely attach the article tether to the article. The user may then fasten the clasp to their apparel so as to prevent the article from getting misplaced when not in use.

Abstract: A two-step method for cloning restriction modification systems is provided which comprises introducing the modification or methylase gene into a host cell and expressing the gene therein to protect the host cell followed by introducing the restriction or endonuclease gene into the host whereby the restriction gene is expressed at lower levels relative to the modification gene. Related methods for producing restriction enzymes is also disclosed as well as clones containing the BamI and DdeI restriction genes.