Abstract: A method and device for analyzing species in a sample includes immobilized ligands in a porous matrix disposed in a microchannel. A method of analysis is provided by loading a sample to the microchannel and establishing an analyte flow through the porous matrix. The analyte is detected as the species that binds to the ligand immobilized in the porous matrix. The present method may be adapted to identify multiple species present in a sample by immobilizing different ligands in a series in a single microfluidic channel by sequentially photopolymerization of various porous matrixes, each containing different ligands.