Abstract: Disclosed herein is a sample introducing apparatus which is designed such that the analytical flow path runs from the needle to the separation column without the flow path switching means placed at the downstream side of the needle. This design reduces dead volume, which in turn reduces the diffusion of the sample injected into the analytical flow path. Moreover, the absence of the flow path switching means at the downstream side of the needle to inject a sample into the analytical flow path eliminates connection of the pipe with the flow path switching means. This prevents the sample from remaining in the connecting part, thereby reducing sample carry-over and improving the accuracy of analysis.

Abstract: Both a column having high-pressure resistance and capable of performing high-speed separation and analysis even with a small flow rate and a liquid chromatograph apparatus using thereof are to be provided. A separation column according to the present invention has a monolithic rod being arranged in the center, being made of a porous material, and having a circular cross section, a filler layer arranged such that it encircles a circumferential surface of the monolithic rod, and a cylindrical support medium arranged outside the filler layer. The filler layer is formed by filling a tube-like gap between the monolithic rod and the support medium with particles or beads.

Abstract: Disclosed herein is a separation column comprising a porous block covered with a coating wherein space-free adhesion is attainable therebetween by low temperature process without releasing octadecylsilyl bases and others from the porous block. A monolith rod 1 is covered with a metal or resin coating 2. Then, the monolith rod 1 covered by the coating 2 is further covered with a rod retainer 3 made of a low melting point metal (melting point 100-200 degrees C.) to form a column body 19. Since the rod retainer 3 is formed by pressure molding at low temperature (100-200 degrees C.). It is therefore possible to absorb the surface irregularities of the monolith rod 1 by the coating 2 while octadecylsilyl bases and others from are released when the retainer 3 is formed. This allows the potential separation ability of the porous block to be brought out maximally.

Abstract: A pump device is realized, which accurately detects a flow rate of a pump, performs real-time control of the flow rate, and thus can suppress a flow rate error caused by occurrence of pulsation. Control of a pump rotation speed by a pressure sensor has low responsiveness in a low pressure region, and a correlation between a pressure and a flow rate is incomplete in a high flow rate region. With this taken into consideration, a flow rate sensor 10 is disposed at an outlet port 9 of a second pump 4. A position detection sensor 14 detects a point where a first pump 3 is shifted from a suction mode to a discharge mode. Moreover, the flow rate sensor 10 detects that there is no discharge from the pump 3, and that there is discharge from the pump 4 alone. Accordingly, a controller 15 sets a rotation speed of a motor 12 to a speed double the previous speed (double the normal rotation speed).

Abstract: In a chromatograph comprising a pump, a sample injecting unit, a column, a photo-diode array detector and a data processing unit having a spectrum library of registered spectrums of known components, a spectrum selected in advance from the spectrum library is compared with a spectrum of each of peaks in an actual sample. Thereby, it is possible to reduce the number of spectrum comparisons required for identification, and it is further possible to perform identification quickly since the identification can be performed during analysis.

Abstract: In a chromatograph comprising a pump, a sample injecting unit, a column, a photo-diode array detector and a data processing unit having a spectrum library of registered spectrums of known components, a spectrum selected in advance from the spectrum library is compared with a spectrum of each of the peaks in an actual sample. Thereby, it is possible to reduce the number of spectrum comparisons required for identification, and it is further possible to perform identification quickly since the identification can be performed during analysis.