Patents by Inventor Kinya Fujishiro

Kinya Fujishiro has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cholesterol oxidase
    Patent number: 6410253
    Abstract: A process for producing cholesterol oxidase from a recombinant microorganism, the microorganism itself and its recombinant plasmid are disclosed. The cholesterol oxidase has a particular amino acid sequence, a high substrate affinity and a working pH in the acidic range. The gene for this cholesterol oxidase was derived from Brevibacterium sterolicum. The cholesterol oxidase is used for the quantitative determination of cholesterol.
    Type: Grant
    Filed: August 14, 1998
    Date of Patent: June 25, 2002
    Assignee: Kyowa Hakko Kogyo Co., Ltd.
    Inventors: Kinya Fujishiro, Takayuki Uwajima
  • Determining ascorbic acid using ASOD and LEUCO chromogen
    Patent number: 6153399
    Abstract: A reagent, kit and method for the quantative determination of ascorbic acid in a sample uses ascorbate oxidase which catalyzes the reaction of reduced ascorbic acid to oxidized ascorbic acid and hydrogen peroxide. Ascorbic acid in the sample is reacted with oxygen in the presence of the ascorbate oxidase, chromogen and peroxidase. Absorbance is determined and compared with a known calibration curve.
    Type: Grant
    Filed: September 18, 1998
    Date of Patent: November 28, 2000
    Assignee: Kyowa Medex Co., Ltd.
    Inventors: Kinya Fujishiro, Katsuyuki Taga, Norihito Aoyama, Akira Miike
  • Cholesterol oxidase
    Patent number: 5798242
    Abstract: A cholesterol oxidase having a particular amino acid sequence and having a high substrate affinity and a working pH in an acidic range is produced by Brevibacterium sterolicum.
    Type: Grant
    Filed: December 5, 1996
    Date of Patent: August 25, 1998
    Assignee: Kyowa Hakko Kogyo Co., Ltd.
    Inventors: Kinya Fujishiro, Takayuki Uwajima
  • Cholesterol oxidase
    Patent number: 5665560
    Abstract: A process for producing cholesterol oxidase from a recombinant microorganism, the microorganism itself and its recombinant plasmid are disclosed. The cholesterol oxidase has a particular amino acid sequence, a high substrate affinity and a working pH in the acidic range. The gene for this cholesterol oxidase was derived from Brevibacterium sterolicum. The cholesterol oxidase is used for the quantitative determination of cholesterol.
    Type: Grant
    Filed: June 2, 1995
    Date of Patent: September 9, 1997
    Assignee: Kyowa Hakko Kogyo Co., Ltd.
    Inventors: Kinya Fujishiro, Takayuki Uwajima
  • Cholesterol oxidase
    Patent number: 5602017
    Abstract: A process for producing cholesterol oxidase from a recombinant microorganism, the microorganism itself and its recombinant plasmid are disclosed. The cholesterol oxidase has a particular amino acid sequence, a high substrate affinity and a working pH in the acidic range. The gene for this cholesterol oxidase was derived from Brevibacterium sterolicum.
    Type: Grant
    Filed: June 17, 1994
    Date of Patent: February 11, 1997
    Assignee: Kyowa Hakko Kogyo Co., Ltd.
    Inventors: Kinya Fujishiro, Takayuki Uwajima
  • Cholesterol oxidase
    Patent number: 5371005
    Abstract: A cholesterol oxidase having a particular amino acid sequence and having a high substrate affinity and a working pH in an acidic range is produced by Brevibacterium sterolicum.
    Type: Grant
    Filed: February 8, 1993
    Date of Patent: December 6, 1994
    Assignee: Kyowa Hakko Kogyo Co., Ltd.
    Inventors: Kinya Fujishiro, Takayuki Uwajima
  • Novel acetylpolyamine amidohydrolase
    Patent number: 4981788
    Abstract: A novel acetylpolyamine amidohydrolase is described. The enzyme specifically hydrolyzes the amino bond in acetylputrescine, acetylcadaverine, acetylspermidine and acetylspermine with strong substrate affinity. The enzyme is preferably produced by culturing a microorganism belonging to the genus Mycoplana, and is used in the quantitative determination of polyamine contained in a living body sample, which is useful for cancer diagnosis.
    Type: Grant
    Filed: June 28, 1988
    Date of Patent: January 1, 1991
    Assignee: Kyowa Hakko Kogyo Co., Ltd.
    Inventors: Takayuki Uwajima, Kinya Fujishiro, Mayumi Ando
  • Novel aldehyde oxidase and process for producing same
    Patent number: 4666842
    Abstract: Disclosed is a new enzyme, aldehyde oxidase, which catalyzes the oxidation of an aldehyde to a corresponding acid but does not have any catalytic action upon other substrates. Also disclosed is a fermentative process from producing the enzyme.
    Type: Grant
    Filed: January 9, 1986
    Date of Patent: May 19, 1987
    Assignee: Kyowa Hakko Kogyo Co., Inc.
    Inventors: Takayuki Uwajima, Kinya Fujishiro, Kenichiro Takayama