Abstract: The invention relates to biosensors. More particularly, this invention relates to an electrochemical biosensor and to electrochemically active enzymes or variants thereof that are suitable for detection of one or more target molecules in a sample.

Abstract: Sense codon reassignment to unnatural amino acids (uAAs) represents a powerful approach for introducing novel properties into polypeptides. The main obstacle to this approach is competition between the native isoacceptor tRNA(s) and orthogonal tRNA(s) for the reassigned codon. While several chromatographic and enzymatic procedures for selective deactivation of tRNA isoacceptors in cell-free translation systems exist, they are complex and not scalable. The present invention provides oligonucleotides that hybridise to a tRNA of interest when said tRNA is in a folded state, thereby disrupting the function of the tRNA. The present invention also provides the use of these oligonucleotides in methods for sense codon reassignment and methods for incorporation of uAAs into proteins. The approach described herein represents a new direction in genetic code reassignment with numerous practical applications.

Abstract: The invention relates to biosensors. More particularly, this invention relates to an electrochemical biosensor and to electrochemically active enzymes or variants thereof that are suitable for detection of one or more target molecules in a sample.

Abstract: A biosensor comprises an amino acid sequence of an enzyme such as glucose dehydrogenase which is capable of reacting with a substrate to produce one or more electrons, wherein the enzyme has been engineered to be switchable from a catalytically inactive to a catalytically active state in response to binding a target molecule. A method of detecting a target molecule is provided wherein an enzyme such as glucose dehydrogenase reacts with a substrate to produce one or more electrons as a result of the enzyme switching from a catalytically inactive to a catalytically active state in response to binding the target molecule.

Abstract: A complement to tRNAs and a protein translation system are provided that enable the incorporation of non-natural moieties such as non-natural amino acids without compromising the ability to incorporate all of the twenty natural amino acids into the protein. This is achieved by reassigning one of the tRNA anticodons for amino acids that are normally decoded by at least two different tRNA anticodons to a non-natural moiety, wherein at least one codon can be uniquely recognized by the reassigned anticodon and at least one another codon from the same codon box cannot be recognized by the reassigned anticodon. Accordingly, an mRNA for translation is engineered to comprise one or more specific codons corresponding to the reassigned tRNA anticodons so that the non-natural moiety is incorporated into the translated protein at a selected position.

Abstract: System and method for establishing configuration parameters for a comprehensive user interface of an alarm system. An exemplary embodiment of a configuration system in accordance with the present disclosure includes a plurality of alarm system workstations, each alarm system workstation having a set of configuration parameters associated therewith, a client workstation, and a user interface broker operatively connected to each of the alarm system workstations and the client workstation, wherein the user interface broker is configured to aggregate data from the alarm system workstations and to present such aggregated data at the client workstation using the comprehensive user interface. The user interface broker is further configured to facilitate designation of one of the alarm system workstations as a main workstation, whereupon the configuration parameters of the designated main workstation are applied to the comprehensive user interface.

Abstract: A biosensor comprises first and second molecular components and is capable of displaying non-protease enzyme activity in response to a binding event mediated by first and second binding partners of the biosensor. The first and second binding partners may bind each other directly or may both bind a target molecule. At least the first molecular component comprises an inhibited non-protease enzyme, whereby the binding event switches the enzyme from a catalytically inactive state to an active state. The second molecular component may comprise a protease that cleaves the first molecular component to release inhibition of the non-protease enzyme of first molecular component. Alternatively, the second molecular component may comprise a trap molecule that binds a bait molecule of the first molecular component to release inhibition of the non-protease enzyme of first molecular component.

Abstract: A biosensor molecule comprises: a protease amino acid sequence; at least one sensor comprising at least one sensor amino acid sequence which is responsive to at least one target molecule; and an inhibitor of the protease activity of said protease amino acid sequence; wherein the biosensor is switchable from a protease active to a protease inactive state, or from a protease inactive to a protease active state when said sensor responds to said target molecule. The biosensor protease may be a protease of a virus such as a Potyvirus or a Flavivirus wherein the inhibitor is an autoinhibitory peptide derived from the virus. The biosensor may respond to the target molecule allosterically or may be cleaved by a target protease molecule.

Abstract: A biosensor comprises first and second molecular components and is capable of displaying protease activity in response to a binding event mediated by first and second binding partners of the biosensor. The first and second binding partners may bind each other directly or may both bind a target molecule. At least the first molecular component comprises an autoinhibited protease, whereby the binding event switches the protease frora an autoinhibited inactive state to a protease active state. The second molecular component may activate the protease of the first molecular component by binding a cross-binder which releases the autoinhibitor or by cleaving a linker which releases the autoinhibitor. The first and second molecular components may both have autoinhibited proteases which reciprocally activate each other.

Abstract: A biosensor comprises first and second molecular components and is capable of displaying protease activity in response to a binding event mediated by first and second binding partners of the biosensor. The first and second binding partners may bind each other directly or may both bind a target molecule. At least the first molecular component comprises an autoinhibited protease, whereby the binding event switches the protease frora an autoinhibited inactive state to a protease active state. The second molecular component may activate the protease of the first molecular component by binding a cross-binder which releases the autoinhibitor or by cleaving a linker which releases the autoinhibitor. The first and second molecular components may both have autoinhibited proteases which reciprocally activate each other.

Abstract: A biosensor molecule comprises: a protease amino acid sequence; at least one sensor comprising at least one sensor amino acid sequence which is responsive to at least one target molecule; and an inhibitor of the protease activity of said protease amino acid sequence; wherein the biosensor is switchable from a protease active to a protease inactive state, or from a protease inactive to a protease active state when said sensor responds to said target molecule. The biosensor protease may be a protease of a virus such as a Potyvirus or a Flavivirus wherein the inhibitor is an autoinhibitory peptide derived from the virus. The biosensor may respond to the target molecule allosterically or may be cleaved by a target protease molecule.

Abstract: An interface system for providing a comprehensive user interface for alarm systems. The interface system may include two or more alarm system workstations, each having a user interface application installed thereon. One or more alarm panels may be connected to each of the alarm system workstations, and one or more points may be connected to each of the alarm panels. A broker workstation may be connected to the two or more alarm system workstations, and one or more client workstations may be connected to the broker workstation. A user interface broker may be installed on the broker workstation, wherein the user interface broker is configured to provide the one or more client workstations with a user interface that presents the status of, and that provides control over, all of the points.

Abstract: System and method for establishing configuration parameters for a comprehensive user interface of an alarm system. An exemplary embodiment of a configuration system in accordance with the present disclosure includes a plurality of alarm system workstations, each alarm system workstation having a set of configuration parameters associated therewith, a client workstation, and a user interface broker operatively connected to each of the alarm system workstations and the client workstation, wherein the user interface broker is configured to aggregate data from the alarm system workstations and to present such aggregated data at the client workstation using the comprehensive user interface. The user interface broker is further configured to facilitate designation of one of the alarm system workstations as a main workstation, whereupon the configuration parameters of the designated main workstation are applied to the comprehensive user interface.

Abstract: An interface system for providing a comprehensive user interface for alarm systems. The interface system may include two or more alarm system workstations, each having a user interface application installed thereon. One or more alarm panels may be connected to each of the alarm system workstations, and one or more points may be connected to each of the alarm panels. A broker workstation may be connected to the two or more alarm system workstations, and one or more client workstations may be connected to the broker workstation. A user interface broker may be installed on the broker workstation, wherein the user interface broker is configured to provide the one or more client workstations with a user interface that presents the status of, and that provides control over, all of the points.