Patents by Inventor Kirston Koths

Kirston Koths has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Compositions containing reduced, microbially produced interleukin-2 (IL-2)
    Patent number: 5614185
    Abstract: A process for recovering microbially produced IL-2 in a highly pure form from the cellular material of the microorganisms that produced it comprising: disrupting the-cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.
    Type: Grant
    Filed: April 25, 1995
    Date of Patent: March 25, 1997
    Assignee: Chiron Corporation
    Inventors: Kirston Koths, James Thomson, Michael Kunitani, Kenneth Wilson, Wolf Hanisch
  • Purified, reduced recombinant interleukin-2 compositions
    Patent number: 5419899
    Abstract: A process for recovering microbially produced IL-2 in a highly pure form from the cellular material of the microorganisms that produced it comprising: disrupting the cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction, mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.
    Type: Grant
    Filed: May 6, 1992
    Date of Patent: May 30, 1995
    Inventors: Kirston Koths, James Thomson, Michael Kunitani, Kenneth Wilson, Wolf Hanisch
  • Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
    Patent number: 4569790
    Abstract: A process for recovering microbially produced IL-2 in a highly pure form from the cellular material of the microorganisms that produced it comprising: disrupting the cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.
    Type: Grant
    Filed: March 28, 1984
    Date of Patent: February 11, 1986
    Assignee: Cetus Corporation
    Inventors: Kirston Koths, James Thomson, Michael Kunitani, Kenneth Wilson, Wolf Hanisch