Abstract: In a liquid chromatograph, the disulfide adsorption of iron atoms in a filter for a column is avoided and thus peak tailing in a chromatogram is prevented to thereby prevent a lowering in the sample recovery rate. A liquid chromatograph column comprising a hollow tubular column body, a bead-shaped packing material which is packed in the hollow part of the column body, a filter provided with a mesh which has a mesh size smaller than the particle diameter of the packing material, and a cap which presses the filter against the packing material to thereby enclose the packing material within the column body, characterized in that the main component of the filter is nickel or hastelloy.

Abstract: Provided are an adsorptive carbon which can effectively adsorb vivotoxins such as advanced glycation end products (AGEs), and an adsorbent containing the adsorptive carbon as an active ingredient. The adsorptive carbon according to the present invention has a total pore volume of 0.10 to 1.0 mL/g, an average pore diameter of 1.0 to 2.0 nm, and an absorbance of an infrared absorption band at 1650-1800 cm?1 of no less than 0.005.

Abstract: It is possible to correctly determine whether a change in the pressure or flow rate is caused by normal opening and closing operations of a check valve and to monitor in real time whether an operation of a check valve is normal or abnormal in a liquid supply device. Light is introduced in the check valve and a change in the quantity of light transmitted through or reflected by the check valve, caused by the opening and closing of the valve is detected, so that the opening and closing operations of the check valve can be directly detected. The check valve is arranged in a pipe in the liquid supply device, and a change in the pressure in the pipe is monitored on the basis of a signal from a pressure sensor and a signal which represents the opening and closing of the check valve.

Abstract: Both a column having high-pressure resistance and capable of performing high-speed separation and analysis even with a small flow rate and a liquid chromatograph apparatus using the column are provided. A separation column according to the present invention has a monolithic rod being arranged in the center, being made of a porous material, and having a circular cross section, a filler layer arranged such that it encircles a circumferential surface of the monolithic rod, and a cylindrical support medium arranged outside the filler layer. The filler layer is formed by filling a tube-like gap between the monolithic rod and the support medium with particles or beads.

Abstract: Provided are an adsorptive carbon which can effectively adsorb vivotoxins such as advanced glycation end products (AGEs), and an adsorbent containing the adsorptive carbon as an active ingredient. The adsorptive carbon according to the present invention has a total pore volume of 0.10 to 1.0 mL/g, an average pore diameter of 1.0 to 2.0 nm, and an absorbance of an infrared absorption band at 1650-1800 cm?1 of no less than 0.005.

Abstract: After various sugar nucleotide solutions and glycosyltransferases (or primers) have been mixed, they are introduced into a reaction tank (column) with primers (or glycosyltransferases) immobilized thereon. Then solutions coming out of the reaction tank are led to an ultrafiltration column. The oligosaccharide synthesizer according to the present invention is equipped with a flow path for ensuring that glycosyltransferases or primers separated by the ultrafiltration column are returned into a container for storing each solution in a sample injector.

Abstract: A chromatographic analyzer is provided for facilitating curve fitting by means of the linear least-square method for a chromatogram that contains a plurality of overlapping peaks. The present invention is characterized by a chromatographic data processor for executing data processing of a chromatogram obtained by separating a sample to be measured using a column and detecting the separated sample, wherein fitting processing is executed to each peak in an arbitrary time region having the plurality of peaks of the chromatogram starting from the front side of the time region or from the back side of the time region, and the processed peaks are subtracted from the chromatogram in the time region so that the plurality of peaks in the chromatogram can be separated from one another. Thus, the plurality of overlapping peaks, particularly three or more overlapping peaks in the chromatogram can be easily separated from one another only by defining some setting conditions.

Abstract: It is possible to correctly determine whether a change in the pressure or flow rate is caused by normal opening and closing operations of a check valve and to monitor in real time whether an operation of a check valve is normal or abnormal in a liquid supply device. Light is introduced in the check valve and a change in the quantity of light transmitted through or reflected by the check valve, caused by the opening and closing of the valve is detected, so that the opening and closing operations of the check valve can be directly detected. The check valve is arranged in a pipe in the liquid supply device, and a change in the pressure in the pipe is monitored on the basis of a signal from a pressure sensor and a signal which represents the opening and closing of the check valve.

Abstract: In a liquid delivery device, when an inlet-side check valve and an outlet-side check valve of a first cylinder are both closed, and compression of an eluent inside the first cylinder is started by means of a first plunger inside the first cylinder, a control unit doubles the rotational speed of a step motor, and measures the amount of change in the pressure inside the first cylinder which changes for a prescribed time by means of a first cylinder internal pressure detector. The time elapsed until the pressure inside the first cylinder is the same as the pressure inside a discharge-side flow passage is predicted using the rate of change over time of the pressure of the eluent. When the predicted elapsed time has elapsed, the rotational speed of the step motor is returned to the original speed, and the compression of the eluent inside the first cylinder is completed.

Abstract: In a liquid chromatograph, the disulfide adsorption of iron atoms in a filter for a column is avoided and thus peak tailing in a chromatogram is prevented to thereby prevent a lowering in the sample recovery rate. A liquid chromatograph column comprising a hollow tubular column body, a bead-shaped packing material which is packed in the hollow part of the column body, a filter provided with a mesh which has a mesh size smaller than the particle diameter of the packing material, and a cap which presses the filter against the packing material to thereby enclose the packing material within the column body, characterized in that the main component of the filter is nickel or hastelloy.

Abstract: A chromatographic analyzer is provided for facilitating curve fitting by means of the linear least-square method for a chromatogram that contains a plurality of overlapping peaks. A chromatographic data processor executes fitting processing to each peak in an arbitrary time region having the plurality of peaks of the chromatogram starting from the front or back side of the time region or from the back side of the time region, and the processed peaks are subtracted from the chromatogram in the time region so that the plurality of peaks in the chromatogram can be separated from one another.

Abstract: A separation analyzer for transferring solutions, while changing mixing ratios of the solutions, wherein a flow passage from a first pump, a flow passage from a second pump, and a passage for sample introduction section, the flow passages being connected, which further comprises a flow switching means for temporarily storing the solutions, whereby the flow switching means alternatively makes a first state for pushing out the solutions in a second sampling loop to the sample introduction section by the second pump, while transferring the solutions to the first sampling loop; and a second state for pushing out the solutions in the first sampling loop by the second pump, while transferring the solutions from the first pump to the second sampling loop.

Abstract: A method for analyzing a multi-channel chromatogram is realized for accurately resolving overlapping peaks on a multi-channel chromatogram to permit analysis of the composition of a sample. First, components of overlapping peaks are specified. A data matrix Dij is determined, and compressed in the wavelength direction to obtain a data matrix vector Di. Next, the two-dimensional data is deconvoluted, final standard deviation s0 is registered, and the deconvoluted two-dimensional data is reconvoluted to a retention intensity matrix. A quantitative spectral intensity matrix is computed, components are identified, and the identified components are quantified. Subsequently, an eigenvalue problem is solved, and the number n of components is estimated. The deconvolution and computation of the eigenvalue problem are iteratively executed until the peaks are isolated. Then, elution profiles are calculated, the components are identified, and the identified components are quantified.

Abstract: A chromatographic analyzer is provided for facilitating curve fitting by means of the linear least-square method for a chromatogram that contains a plurality of overlapping peaks. The present invention is characterized by a chromatographic data processor for executing data processing of a chromatogram obtained by separating a sample to be measured using a column and detecting the separated sample, wherein fitting processing is executed to each peak in an arbitrary time region having the plurality of peaks of the chromatogram starting from the front side of the time region or from the back side of the time region, and the processed peaks are subtracted from the chromatogram in the time region so that the plurality of peaks in the chromatogram can be separated from one another. Thus, the plurality of overlapping peaks, particularly three or more overlapping peaks in the chromatogram can be easily separated from one another only by defining some setting conditions.

Abstract: In a liquid chromatography apparatus, a separation column of intermediate stage is additionally connected between a separation column of first stage and a separation column of second stage. Preferably, a switching unit and a liquid feed unit for mixing and feeding a plurality of solutions are added to improve a separation capability. A three-dimensional liquid chromatography apparatus capable of avoiding the “solution interference” can be realized. Even a complex sample containing a hydrophilic component and a hydrophobic component in a mixed state can be separated and analyzed satisfactorily on-line.

Abstract: The present invention provides a sugar chain synthesizer capable of continuously reacting sugar chains when a plurality of sugar chains are successively reacted. The sugar chain synthesizer of the present invention includes a plurality of vessels containing respective sugar nucleotide solutions, a plurality of vessels containing respective glycosyltransferases, and a reactor containing a primer that is a water-soluble polymer, into which the above described sugar nucleotide solution and glycosyltransferase are introduced. In the present invention, components in a reaction solution obtained in the reactor are separated through an ultrafiltration column, and a reaction product is then returned to the above described reactor, so as to continuously synthesize sugar chains. Although it is a complicated synthesis of sugar chains, it becomes possible to carry out such synthesis continuously and automatically.

Abstract: To analyze glycans and peptide sequences without liberating glycans from glycosylated peptides, a glycan structure is analyzed through negative-ion CID, in which sialic acid and fucose are resistant to elimination, and a peptide sequence is analyzed through positive-ion ECD.

Abstract: A separation analyzer for transferring solutions, while changing mixing ratios of the solutions, wherein a flow passage from a first pump, a flow passage from a second pump, and a passage for sample introduction section, the flow passages being connected, which further comprises a flow switching means for temporarily storing the solutions, whereby the flow switching means alternatively makes a first state for pushing out the solutions in a second sampling loop to the sample introduction section by the second pump, while transferring the solutions to the first sampling loop; and a second state for pushing out the solutions in the first sampling loop by the second pump, while transferring the solutions from the first pump to the second sampling loop.

Abstract: A separation analyzer for transferring solutions, while changing mixing ratios of the solutions, wherein a flow passage from a first pump, a flow passage from a second pump, and a passage for sample introduction section, the flow passages being connected, which further comprises a flow switching means for temporarily storing the solutions, whereby the flow switching means alternatively makes a first state for pushing out the solutions in a second sampling loop to the sample introduction section by the second pump, while transferring the solutions to the first sampling loop; and a second state for pushing out the solutions in the first sampling loop by the second pump, while transferring the solutions from the first pump to the second sampling loop.

Abstract: A chromatographic analyzer is provided for facilitating curve fitting by means of the linear least-square method for a chromatogram that contains a plurality of overlapping peaks. The present invention is characterized by a chromatographic data processor for executing data processing of a chromatogram obtained by separating a sample to be measured using a column and detecting the separated sample, wherein fitting processing is executed to each peak in an arbitrary time region having the plurality of peaks of the chromatogram starting from the front side of the time region or from the back side of the time region, and the processed peaks are subtracted from the chromatogram in the time region so that the plurality of peaks in the chromatogram can be separated from one another. Thus, the plurality of overlapping peaks, particularly three or more overlapping peaks in the chromatogram can be easily separated from one another only by defining some setting conditions.