Patents by Inventor Kithmie Harshana Malagodapathiranage

Kithmie Harshana Malagodapathiranage has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • NOVEL ENZYMATIC METHODS TO GENERATE HIGH YIELDS OF SEQUENCE SPECIFIC RNA OLIGONUCLEOTIDES WITH EXTREME PRECISION
    Publication number: 20230265477
    Abstract: Described herein are synthetic methods for producing sequence-specific RNA oligonucleotides that eliminate impurities produced in prior art methods. In one aspect, an end-protected capture DNA complementary to a portion of the product RNA is employed. In another aspect, the template DNA is covalently or noncovalently linked to the RNA polymerase, either directly or through the use of a nontemplate DNA. In a third aspect, a flow chamber is employed. All of the methods can be used in combination.
    Type: Application
    Filed: January 10, 2023
    Publication date: August 24, 2023
    Inventors: Craig Martin, Elvan Cavac, Kithmie Harshana Malagodapathiranage, Shuo Sui, Sarah L. Perry, Yasaman Gholamalipour
  • Enzymatic methods to generate high yields of sequence specific rna with extreme precision
    Patent number: 11578348
    Abstract: Described herein are synthetic methods for producing sequence-specific RNA oligonucleotides that eliminate impurities produced in prior art methods. In one aspect, an end-protected capture DNA complementary to a portion of the product RNA is employed. In another aspect, the template DNA is covalently or noncovalently linked to the RNA polymerase, either directly or through the use of a nontemplate DNA. In a third aspect, a flow chamber is employed. All of the methods can be used in combination.
    Type: Grant
    Filed: April 24, 2020
    Date of Patent: February 14, 2023
    Assignee: THE UNIVERSITY OF MASSACHUSETTS
    Inventors: Craig Martin, Elvan Cavac, Kithmie Harshana Malagodapathiranage, Shuo Sui, Sarah L. Perry, Yasaman Gholamalipour
  • NOVEL ENZYMATIC METHODS TO GENERATE HIGH YIELDS OF SEQUENCE SPECIFIC RNAS WITH EXTREME PRECISION
    Publication number: 20220112546
    Abstract: Described herein are synthetic methods for producing sequence-specific RNA oligonucleotides that eliminate impurities produced in prior art methods. In one aspect, a first amplification primer includes one or more deoxyuridine residues, wherein at least one of the one or more deoxyuridine residues is at position ?1, ?2, ?3, ?4 or ?5 of the promoter region for the single-subunit, DNA-dependent RNA polymerase. The deoxyuridines are excised to provide an amplified functional template DNA which is then used to synthesize RNA which has reduced immunogenic double stranded RNA compared to controls.
    Type: Application
    Filed: October 28, 2021
    Publication date: April 14, 2022
    Inventors: Craig Martin, Elvan Cavac, Kithmie Harshana Malagodapathiranage
  • NOVEL ENZYMATIC METHODS TO GENERATE HIGH YIELDS OF SEQUENCE SPECIFIC RNA OLIGONUCLEOTIDES WITH EXTREME PRECISION
    Publication number: 20200340028
    Abstract: Described herein are synthetic methods for producing sequence-specific RNA oligonucleotides that eliminate impurities produced in prior art methods. In one aspect, an end-protected capture DNA complementary to a portion of the product RNA is employed. In another aspect, the template DNA is covalently or noncovalently linked to the RNA polymerase, either directly or through the use of a nontemplate DNA. In a third aspect, a flow chamber is employed. All of the methods can be used in combination.
    Type: Application
    Filed: April 24, 2020
    Publication date: October 29, 2020
    Inventors: Craig Martin, Elvan Cavac, Kithmie Harshana Malagodapathiranage, Shuo Sui, Sarah L. Perry, Yasaman Gholamalipour