Patents by Inventor Kiyochika Tokuyasu

Kiyochika Tokuyasu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for extracting and purifying kallidinogenase
    Patent number: 4038141
    Abstract: A method for extracting and collecting kallidinogenase (EC 3.4.4.21 substance) which comprises extracting the pancreas of a mammal with water in the presence of a salt at a temperature of about 45.degree. to about 60.degree. C. and a pH of about 5 to about 8, wherein the extraction is carried out in the presence of a water-soluble salt of magnesium with an acid, and a method for purifying kallidinogenase which comprises subjecting the aqueous phase of a kallidinogenase-containing aqueous extract obtained from the pancreas of a mammal to an adsorption-elution treatment using an anion-exchange resin, wherein said aqueous phase of the kallidinogenase-containing aqueous extract is brought into contact with a gel-type weakly basic anion-exchange resin to cause the kallidinogenase fraction to be adsorbed to said resin, and the kallidinogenase is eluted with an aqueous solution of an ammonium salt of a weak acid, thereby to collect the kallidinogenase.
    Type: Grant
    Filed: April 7, 1976
    Date of Patent: July 26, 1977
    Assignee: Seikagaku Kogyo Co. Ltd.
    Inventors: Kiyochika Tokuyasu, Takeshi Yokobori, Chizuko Nakahara
  • Methods for extracting and purifying kallidinogenase
    Patent number: 3994782
    Abstract: A method for extracting and collecting kallidinogenase (EC 3.4.4.21 substance) which comprises extracting the pancreas of a mammal with water in the presence of a salt at a temperature of about 45.degree. to about 60.degree. C. and a pH of about 5 to about 8, wherein the extraction is carried out in the presence of a water-soluble salt of magnesium with an acid, and a method for purifying kallidinogenase which comprises subjecting the aqueous phase of a kallidinogenase-containing aqueous extract obtained from the pancreas of a mammal to an adsorption-elution treatment using an anion-exchange resin, wherein said aqueous phase of the kallidinogenase-containing aqueous extract is brought into contact with a gel-type weakly basic anion-exchange resin to cause the kallidinogenase fraction to be adsorbed to said resin, and the kallidinogenase is eluted with an aqueous solution of an ammonium salt of a weak acid, thereby to collect the kallidinogenase.
    Type: Grant
    Filed: December 5, 1975
    Date of Patent: November 30, 1976
    Assignee: Seikagaku Kogyo Co. Ltd.
    Inventors: Kiyochika Tokuyasu, Takeshi Yokobori, Chizuko Nakahara