Patents by Inventor Kiyomi SHITAOKA

Kiyomi SHITAOKA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for amplifying a T cell receptor (TCR) cDNA
    Patent number: 10533204
    Abstract: As a method for highly efficiently amplifying a TCR cDNA in a short period of time, there is provided a method for amplifying a T cell receptor (TCR) cDNA, which comprises the following step (1) and step (2): (1) the step of performing PCR by using at least one kind of the L primer mentioned below, the C primer 1 or UTR primer 1 mentioned below, and cDNA obtained from a single cell as the template to obtain an amplification product 1; an L primer of 30- to 60-nucleotide length comprising an adapter part of 15- to 25-nucleotide length, and a leader region-annealing part of 15- to 25-nucleotide length, which is ligated downstream from the adapter part, and can anneal to a part of a leader region containing a translation initiation codon, or an upstream part thereof, a C primer 1 of 15- to 25-nucleotide length, which can anneal to a part of a constant region, or a UTR primer 1 of 15- to 25-nucleotide length, which can anneal to a part of a 3? untranslated region; (2) the step of performing PCR by using the a
    Type: Grant
    Filed: May 29, 2015
    Date of Patent: January 14, 2020
    Assignees: National University Corporatlon University of Toyama, SC World, Inc.
    Inventors: Hiroshi Hamana, Hiroyuki Kishi, Atsushi Muraguchi, Kiyomi Shitaoka
  • Method for Amplifying TCR cDNA
    Publication number: 20170183700
    Abstract: As a method for highly efficiently amplifying a TCR cDNA in a short period of time, there is provided a method for amplifying a T cell receptor (TCR) cDNA, which comprises the following step (1) and step (2): (1) the step of performing PCR by using at least one kind of the L primer mentioned below, the C primer 1 or UTR primer 1 mentioned below, and cDNA obtained from a single cell as the template to obtain an amplification product 1; an L primer of 30- to 60-nucleotide length comprising an adapter part of 15- to 25-nucleotide length, and a leader region-annealing part of 15- to 25-nucleotide length, which is ligated downstream from the adapter part, and can anneal to a part of a leader region containing a translation initiation codon, or an upstream part thereof, a C primer 1 of 15- to 25-nucleotide length, which can anneal to a part of a constant region, or a UTR primer 1 of 15- to 25-nucleotide length, which can anneal to a part of a 3? untranslated region; (2) the step of performing PCR by using the a
    Type: Application
    Filed: May 29, 2015
    Publication date: June 29, 2017
    Inventors: Hiroshi HAMANA, Hiroyuki KISHI, Atsushi MURAGUCHI, Kiyomi SHITAOKA