Patents by Inventor Kiyoshi Hayakawa
Kiyoshi Hayakawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240143465Abstract: A semiconductor device includes first and second processor cores configured to perform a lock step operation and including first and second scan chains. The semiconductor device further includes a scan test control unit that controls a scan test of the first and second processor cores using the first and second scan chains, and a start-up control unit that outputs a reset signal for bringing the first and second processor cores into a reset state. The start-up control unit outputs an initialization scan request before the start of a lock step operation, and the scan test control unit performs an initialization scan test operation on the first and second processor cores by using an initialization pattern.Type: ApplicationFiled: August 18, 2023Publication date: May 2, 2024Inventors: Kiyoshi HAYASE, Yuki HAYAKAWA, Toshiyuki KAYA, Kyohei YAMAGUCHI, Takahiro IRITA, Shinichi SHIBAHARA
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Patent number: 11517741Abstract: An electrode for electroporation comprising a plurality of electrode needles, wherein first polarity electrode needles, an electrode needle holding portion, and a syringe holding portion are provided; two or more first polarity electrode needles project from a bottom surface of a lower structural body of an outer frame support of the electrode needle holding portion toward an electroporation target side; the bottom surface of the lower structural body of the outer frame support is provided with a hole for syringe needle insertion and removal communicating with a syringe holding portion side; the syringe holding portion is provided on a side opposite to the electroporation target side of the electrode needle holding portion; and the syringe holding portion has a path for syringe needle insertion and removal, at least a portion of the path for syringe needle insertion and removal being provided with an electro-conductive portion for a second polarity.Type: GrantFiled: February 2, 2017Date of Patent: December 6, 2022Assignees: TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE, NEPA GENE CO., LTD.Inventors: Michinori Kohara, Yasuhiko Hayakawa, Kiyoshi Hayakawa
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Publication number: 20210187276Abstract: An electrode for electroporation comprising a plurality of electrode needles, wherein first polarity electrode needles, an electrode needle holding portion, and a syringe holding portion are provided; two or more first polarity electrode needles project from a bottom surface of a lower structural body of an outer frame support of the electrode needle holding portion toward an electroporation target side; the bottom surface of the lower structural body of the outer frame support is provided with a hole for syringe needle insertion and removal communicating with a syringe holding portion side; the syringe holding portion is provided on a side opposite to the electroporation target side of the electrode needle holding portion; and the syringe holding portion has a path for syringe needle insertion and removal, at least a portion of the path for syringe needle insertion and removal being provided with an electro-conductive portion for a second polarity.Type: ApplicationFiled: February 2, 2017Publication date: June 24, 2021Inventors: Michinori Kohara, Yasuhiko Hayakawa, Kiyoshi Hayakawa
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Patent number: 10633674Abstract: An object is to develop a technology enabling utilization, by only an extremely simple technique, of a technology which is widely applicable to mammals without requiring the utilization of an ES cell, and which involves modifying a certain gene by targeting a certain sequence on a genome (genome editing technology based on ZFN or the like). Provided is a technology for efficiently modifying an arbitrary target gene of a mammal, by immersing a pronuclear stage mammalian zygote with an intact zona pellucida into a solution containing a pair of molecules of mRNA having a certain sequence, and performing electroporation treatment through application of multiple square-wave pulses in three steps with the total electric energy of a first electric pulse adjusted within a predetermined range.Type: GrantFiled: May 27, 2014Date of Patent: April 28, 2020Assignee: NEPA GENE CO., LTD.Inventors: Takehito Kaneko, Tomoji Mashimo, Yasuhiko Hayakawa, Kiyoshi Hayakawa
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Publication number: 20190382792Abstract: An object is to develop a technology enabling utilization, by only an extremely simple technique, of a technology which is widely applicable to mammals without requiring the utilization of an ES cell, and which involves modifying a certain gene by targeting a certain sequence on a genome (genome editing technology based on ZFN or the like). Provided is a technology for efficiently modifying an arbitrary target gene of a mammal, by immersing a pronuclear stage mammalian zygote with an intact zona pellucida into a solution containing a pair of molecules of mRNA having a certain sequence, and performing electroporation treatment through application of multiple square-wave pulses in three steps with the total electric energy of a first electric pulse adjusted within a predetermined range.Type: ApplicationFiled: August 7, 2019Publication date: December 19, 2019Applicant: NEPA GENE CO., LTD.Inventors: Takehito Kaneko, Tomoji Mashimo, Yasuhiko Hayakawa, Kiyoshi Hayakawa
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Patent number: 10004899Abstract: An electric pulse generator for electroporator includes a poring pulse generator, and a transfer pulse generator. The poring generator includes an n-stage Cockroft-Walton circuit, and a first circuit branched from a branching point of wiring on output side of the Cockroft-Walton, the first circuit includes a switching switch that is turned off in high-voltage mode and turned on in low-voltage mode, a voltage value for switching between the modes is in 200 to 1400 V, the first circuit includes a second circuit in which m2 series of m1 series-connected capacitors are connected in parallel, the switch and second circuit are connected in series, and first wiring on output side of the second circuit is merged with second wiring on output side of the Cockroft-Walton on output side of the branching point, and a withstand voltage of the Cockroft-Walton is a value in 1500 to 5000 V in the high-voltage mode.Type: GrantFiled: December 6, 2016Date of Patent: June 26, 2018Assignee: NEPA GENE CO., LTD.Inventors: Yasuhiko Hayakawa, Kiyoshi Hayakawa, Masahiko Sugiyama, Dai Ayusawa, Kensuke Miki
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Publication number: 20170080222Abstract: An electric pulse generator for electroporator includes a poring pulse generator, and a transfer pulse generator. The poring generator includes an n-stage Cockroft-Walton circuit, and a first circuit branched from a branching point of wiring on output side of the Cockroft-Walton, the first circuit includes a switching switch that is turned off in high-voltage mode and turned on in low-voltage mode, a voltage value for switching between the modes is in 200 to 1400 V, the first circuit includes a second circuit in which m2 series of m1 series-connected capacitors are connected in parallel, the switch and second circuit are connected in series, and first wiring on output side of the second circuit is merged with second wiring on output side of the Cockroft-Walton on output side of the branching point, and a withstand voltage of the Cockroft-Walton is a value in 1500 to 5000 V in the high-voltage mode.Type: ApplicationFiled: December 6, 2016Publication date: March 23, 2017Applicant: NEPA GENE CO., LTD.Inventors: Yasuhiko HAYAKAWA, Kiyoshi HAYAKAWA, Masahiko SUGIYAMA, Dai AYUSAWA, Kensuke MIKI
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Publication number: 20160215297Abstract: An object is to develop a technology enabling utilization, by only an extremely simple technique, of a technology which is widely applicable to mammals without requiring the utilization of an ES cell, and which involves modifying a certain gene by targeting a certain sequence on a genome (genome editing technology based on ZFN or the like). Provided is a technology for efficiently modifying an arbitrary target gene of a mammal, by immersing a pronuclear stage mammalian zygote with an intact zona pellucida into a solution containing a pair of molecules of mRNA having a certain sequence, and performing electroporation treatment through application of multiple square-wave pulses in three steps with the total electric energy of a first electric pulse adjusted within a predetermined range.Type: ApplicationFiled: May 27, 2014Publication date: July 28, 2016Applicant: NEPA GENE CO., LTD.Inventors: Takehito KANEKO, Tomoji MASHIMO, Yasuhiko HAYAKAWA, Kiyoshi HAYAKAWA
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Publication number: 20130126334Abstract: The present invention provides a method for manufacturing a glass substrate for a magnetic disk that enables grinding processing with fixed abrasive grains without a decrease of the grinding rate and that can manufacture a high quality glass substrate at low cost. The present invention is a method for manufacturing a glass substrate for a magnetic disk, including a grinding step of grinding a main surface of a glass substrate using a lubricant and a surface plate that has a grinding surface provided with fixed abrasive grains containing diamond particles. In the grinding step, for example, by adding Al2O3, Al3+ is allowed to be contained in the lubricant that is supplied to the surface on which grinding processing is performed of the glass substrate. The lubricant has an Al3+ content preferably in a range of 0.05 g/L to 1.0 g/L.Type: ApplicationFiled: August 31, 2011Publication date: May 23, 2013Inventors: Yoshihiro Tawara, Kiyoshi Hayakawa, Taiji Osada
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Publication number: 20130122592Abstract: Provided is a method for transferring an extraneous gene by an electroporation technique, which is applicable to a wide range of animal cells and is extremely remarkably improved in viability and gene transferring rate. Also provided is a method for transferring an extraneous gene by an electroporation technique with high viability and gene transferring rate even in the case where no specialized transferring buffer is used. Also provided are: a method for transferring an extraneous gene by an electroporation technique, which is remarkably improved in viability and gene transferring rate, the method including continuously applying, to an animal cell, a first electric pulse (strong electric pulse) and a second electric pulse (weak electric pulse) under specific conditions; and a method for transferring an extraneous gene by an electroporation technique, in which a liquid medium capable of being used for culturing of the animal cell is used as a transferring buffer.Type: ApplicationFiled: January 12, 2011Publication date: May 16, 2013Applicant: NEPA GENE CO., LTD.Inventors: Yasuhiko Hayakawa, Kiyoshi Hayakawa
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Patent number: 7735658Abstract: A classification apparatus including a magnetic stirrer composed of main body (1) and rotor (2) and, arranged on the main body of the magnetic stirrer, classification tube (3). The interior of the classification tube is provided with classification compartments (A-E) partitioned from each other by divider plates (5a-5b). The lowermost classification compartment (A) is furnished with gas emission conduit (7). In the classification compartment (A), the rotor (2) of the magnetic stirrer is arranged. From gas supply source (8), a high-pressure gas is fed into the gas emission conduit. By means of the gas emitted from the gas emission conduit, a vortex flow rising from the lowermost classification compartment sequentially toward higher classification compartments is generated in the classification tube.Type: GrantFiled: January 23, 2007Date of Patent: June 15, 2010Assignees: The Doshisha, Kyoto University, Fukujuen Co., Ltd.Inventors: Jusuke Hidaka, Shinzo Yoshikado, Yoshiyuki Shirakawa, Yoshiaki Ito, Kiyoshi Hayakawa, Yuuko Watanabe
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Publication number: 20090078619Abstract: A classification apparatus including a magnetic stirrer composed of main body (1) and rotor (2) and, arranged on the main body of the magnetic stirrer, classification tube (3). The interior of the classification tube is provided with classification compartments (A-E) partitioned from each other by divider plates (5a-5b). The lowermost classification compartment (A) is furnished with gas emission conduit (7). In the classification compartment (A), the rotor (2) of the magnetic stirrer is arranged. From gas supply source (8), a high-pressure gas is fed into the gas emission conduit. By means of the gas emitted from the gas emission conduit, a vortex flow rising from the lowermost classification compartment sequentially toward higher classification compartments is generated in the classification tube.Type: ApplicationFiled: January 23, 2007Publication date: March 26, 2009Applicants: THE DOSHISHA, Kyoto University, Fukujuen Co., Ltd.Inventors: Jusuke Hidaka, Shinzo Yoshikado, Yoshiyuki Shirakawa, Yoshiaki Ito, Kiyoshi Hayakawa, Yuuko Watanabe
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Publication number: 20090035439Abstract: A method capable of manufacturing, by using a ball mill apparatus, powdered tea having the same quality as that manufactured by using a millstone. By using the ball mill apparatus having synthetic resin pots, a powdered tea material and synthetic resin balls are sealed in the pot, and the pot is revolved or revolved and rotated on its axis to crush the powdered tea material.Type: ApplicationFiled: March 31, 2006Publication date: February 5, 2009Inventors: Jusuke Hidaka, Shinzo Yoshikado, Yoshiyuki Shirakawa, Yoshiaki Ito, Kiyoshi Hayakawa, Yuuko Watanabe
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Patent number: 5053293Abstract: A method for the production of a photoelectrochemical cell includes forming an anion-doped long-chain alkylthiophene polymer layer on one surface of a semiconductor directly or through the medium of a coating of a noble metal formed in advance on said one surface, interposing a solid electrolyte between a transparent electrode and the anion-dope long-chain alkylthiophene polymer on the semiconductor, and finally connecting a lead wire to the other surface of the semiconductor. A photoelectrochemical cell is produced by the method described above and consists essentially of a layer of an anion-doped long-chain alkylthiophene polymer formed on one surface of a semiconductor directly or through the medium of a coating of a noble metal formed in advance, a transparent electrode, and a solid electrolyte interposed between the polymer layer and the transparent electrode.Type: GrantFiled: August 17, 1990Date of Patent: October 1, 1991Assignees: Agency of Industrial Science & Technology, Ministry of International Trade & IndustryInventors: Hiromi Yamakita, Masato Tazawa, Kiyoshi Hayakawa, Hiroshi Taoda
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Patent number: 4934003Abstract: A disabled transferring device comprises a stationary base and a rotary base coupled with the stationary base for forming a base assembly. An actuation lever assembly which has an actuation lever having a pivot point in the vicinity of one end and an actuation point at the other end, is pivotally supported on the rotary base of the base assembly. A pivotal support shaft is secured onto the level in the vicinity of the pivot point in such an arrangement that the securing point of the pivotal support shaft is oriented at the pivot point or between the one end and the pivot point with a first distance to the pivot point of the lever. The first distance is much shorted than a second distance between the pivot point and the actuation point. A saddle to mount thereon the disabled is mounted on the top end of the support shaft.Type: GrantFiled: January 31, 1989Date of Patent: June 19, 1990Assignee: Echo CorporationInventors: Kiyoshi Hayakawa, Takao Arai, Osamu Tanaka, Hiroshi Iijima, Hiroki Suzuki, Fumiyuki Matsuno, Kenji Misugi
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Patent number: 4869795Abstract: A fuel gas is produced from an aqueous acetone solution by causing the aqueous acetone solution to contact powdered silicon and exposing the resultant product of this contact to light.Type: GrantFiled: March 7, 1989Date of Patent: September 26, 1989Assignees: Agency of Industrial Science, Ministry of International Trade and IndustryInventors: Hiroshi Taoda, Kiyoshi Hayakawa, Masato Tazawa, Hiromi Yamakita
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Patent number: 4794130Abstract: A shaped article of halogenated polymer which is obtained by causing a halogen to react upon a shaped article of a polymer by irradiation of light is irradiated with light in the presence of an acetylene compound to have the acetylene compound graft polymerized onto the shaped article of polymer.Type: GrantFiled: March 30, 1987Date of Patent: December 27, 1988Assignees: Agency of Industrial Science and Technology, Ministry of International Trade and IndustryInventors: Kiyoshi Hayakawa, Hiromi Yamakita, Masato Tazawa, Hiroshi Taoda
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Patent number: 4647348Abstract: On a silicon semiconductor or a silicon semiconductor having a coating of a noble metal, a polymer film is deposited by immersing the silicon semiconductor or the silicon semiconductor with the noble metal coating in a solution containing a thiophene compound, applying a voltage to the silicon semiconductor as an electrode and, at the same time, exposing the silicon semiconductor to light.Type: GrantFiled: March 12, 1986Date of Patent: March 3, 1987Assignees: Agency of Industrial Science & Technology, Ministry of International Trade and IndustryInventors: Hiromi Yamakita, Kiyoshi Hayakawa, Masato Tazawa, Hiroshi Taoda
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Patent number: 4502930Abstract: A 7-substituted quadricyclene-cyclodextrin inclusion compound is manufactured by irradiating a 7-substituted norbornadiene-cyclodextrin inclusion compound with light.Type: GrantFiled: February 29, 1984Date of Patent: March 5, 1985Assignees: Agency of Industrial Science & Technology, Ministry of International Trade & IndustryInventors: Takaari Yumoto, Kiyoshi Hayakawa, Kaoru Kawase, Hiromi Yamakita, Hiroshi Taoda
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Patent number: 4473484Abstract: A material comprising an organic compound capable of inducing its own photo-isomerization, a photosensitizer, and a side-reaction inhibitor exhibits an ability to convert solar energy into another form of energy and store it in such form.Type: GrantFiled: July 28, 1983Date of Patent: September 25, 1984Assignees: Agency of Industrial Science & Technology, Ministry of International Trade & IndustryInventors: Hiroshi Taoda, Kiyoshi Hayakawa, Kaoru Kawase, Takaari Yumoto, Mineo Kosaka, Tadashi Asahina