Patents by Inventor Kiyoshi Mizuuchi

Kiyoshi Mizuuchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Detection of nucleic acid sequence variations using phase Mu transposase
    Patent number: 7316903
    Abstract: The present invention relates, e.g., to a method of detecting a mismatch in a double stranded nucleic acid target, comprising (a) contacting the target with (i) a Mu-end nucleic acid, and (ii) a phage Mu transposase, under conditions effective for the Mu-end nucleic acid to transpose into the target at about the site of a mismatch, if the target comprises a mismatch, and (b) detecting transposition of the Mu-end DNA into the target, wherein transposition of the Mu-end nucleic acid into the target at a predominant site indicates the presence of a mismatch at that site.
    Type: Grant
    Filed: March 26, 2004
    Date of Patent: January 8, 2008
    Assignee: United States of America as represented by the Department of Health and Human Services
    Inventors: Katsuhiko Yanagihara, Kiyoshi Mizuuchi
  • Detection of nucleic acid sequence variations using phase MU transposase
    Publication number: 20040191821
    Abstract: The present invention relates, e.g., to a method of detecting a mismatch in a double stranded nucleic acid target, comprising (a) contacting the target with (i) a Mu-end nucleic acid, and (ii) a phage Mu transposase, under conditions effective for the Mu-end nucleic acid to transpose into the target at about the site of a mismatch, if the target comprises a mismatch, and (b) detecting transposition of the Mu-end DNA into the target, wherein transposition of the Mu-end nucleic acid into the target at a predominant site indicates the presence of a mismatch at that site.
    Type: Application
    Filed: March 26, 2004
    Publication date: September 30, 2004
    Applicant: The Government of the USA, as represented by the secretary, Dept. of Health and Human Services
    Inventors: Katsuhiko Yanagihara, Kiyoshi Mizuuchi
  • Mini Mu containing plasmid and a method for rapid DNA sequencing
    Patent number: 4716105
    Abstract: The present invention discloses a rapid method of sequencing a relatively large segment of deoxyribonucleic acid. The method in part comprises high frequency insertion of a suitable transposon into a segment of DNA of interest. Preferable use of Mu transposons is described. A plasmid having mini-Mu transposons has been prepared and disclosed.
    Type: Grant
    Filed: December 13, 1984
    Date of Patent: December 29, 1987
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: Kiyoshi Mizuuchi, Michiyo Mizuuchi, Toshiro Adachi