Patents by Inventor Kiyoto Nishiyama
Kiyoto Nishiyama has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200093915Abstract: The present invention provides a vaccine containing virus-like particles derived from virus particles having an envelope, in which a lipid-component content of the virus-like particles is reduced relative to a lipid-component content of the virus particles.Type: ApplicationFiled: November 18, 2019Publication date: March 26, 2020Applicant: KM Biologics Co., Ltd.Inventors: Kazuhiko Kimachi, Motoharu Abe, Kazuyuki Ikeda, Hiroto Onuma, Yukari Tsurudome, Daisuke Ikeno, Kiyoto Nishiyama, Tatsufumi Onchi, Yusuke Ooyama, Issay Asano, Ryoichi Kitano
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Patent number: 10525122Abstract: The present invention provides a vaccine containing virus-like particles derived from virus particles having an envelope, in which a lipid-component content of the virus-like particles is reduced relative to a lipid-component content of the virus particles.Type: GrantFiled: July 15, 2015Date of Patent: January 7, 2020Assignee: KM Biologics Co., Ltd.Inventors: Kazuhiko Kimachi, Motoharu Abe, Kazuyuki Ikeda, Hiroto Onuma, Yukari Tsurudome, Daisuke Ikeno, Kiyoto Nishiyama, Tatsufumi Onchi, Yusuke Ooyama, Issay Asano, Ryoichi Kitano
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Publication number: 20170196965Abstract: The present invention provides a vaccine containing virus-like particles derived from virus particles having an envelope, in which a lipid-component content of the virus-like particles is reduced relative to a lipid-component content of the virus particles.Type: ApplicationFiled: July 15, 2015Publication date: July 13, 2017Applicant: THE CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTEInventors: Kazuhiko KIMACHI, Motoharu ABE, Kazuyuki IKEDA, Hiroto ONUMA, Yukari TSURUDOME, Daisuke IKENO, Kiyoto NISHIYAMA, Tatsufumi ONCHI, Yusuke OOYAMA, Issay ASANO, Ryoichi KITANO
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Patent number: 8883165Abstract: A modified peptide derived from matrix protein 2 (hereinafter also referred to as “M2”), one of surface layer proteins of influenza virus, and a method for utilization of the modified peptide are provided. A peptide (hereinafter also referred to as “M2eC peptide”) that is made up by inserting cysteine residue(s) into a peptide (hereinafter also referred to as “M2e”) consisting of 23 amino acid residues of from positions No. 2 to No.Type: GrantFiled: August 23, 2010Date of Patent: November 11, 2014Assignee: The Chemo-Sero-Therapeutic Research InstituteInventors: Kazuyoshi Kaminaka, Chikateru Nozaki, Junichi Matsuda, Kiyoto Nishiyama
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Publication number: 20120244184Abstract: A modified peptide derived from matrix protein 2 (hereinafter also referred to as “M2”), one of surface layer proteins of influenza virus, and a method for utilization of the modified peptide are provided. A peptide (hereinafter also referred to as “M2eC peptide”) that is made up by inserting cysteine residue(s) into a peptide (hereinafter also referred to as “M2e”) consisting of 23 amino acid residues of from positions No. 2 to No.Type: ApplicationFiled: August 23, 2010Publication date: September 27, 2012Applicant: THE CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTEInventors: Kazuyoshi Kaminaka, Chikateru Nozaki, Junichi Matsuda, Kiyoto Nishiyama
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Patent number: 7883844Abstract: A method for producing influenza virus on large scale is provided. A method for propagating influenza virus which comprises, after removing or decreasing a trypsin inhibitor secreted into culture of MDCK cells (cell line derived from dog kidney) by washing with a culture medium or a buffer, inoculating influenza virus into said cells and culturing said influenza virus-inoculated cells in a culture medium supplemented with trypsin.Type: GrantFiled: May 11, 2007Date of Patent: February 8, 2011Assignee: Juridical Foundation the Chemosero-Therapeutic Research InstituteInventors: Toshinobu Nouchi, Kiyoto Nishiyama, Keishin Sugawara
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Publication number: 20090181446Abstract: A method for producing influenza virus on large scale is provided. A method for propagating influenza virus which comprises, after removing or decreasing a trypsin inhibitor secreted into culture of MDCK cells (cell line derived from dog kidney) by washing with a culture medium or a buffer, inoculating influenza virus into said cells and culturing said influenza virus-inoculated cells in a culture medium supplemented with trypsin.Type: ApplicationFiled: May 11, 2007Publication date: July 16, 2009Applicant: Juridical Foundation the Chemo-Sero-Therapeutic Research InstituteInventors: Toshinobu Nouchi, Kiyoto Nishiyama, Keishin Sugawara
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Patent number: 5785968Abstract: Anti-FCV (feline calicivirus) feline-type recombinant antibody effective for treatment, prevention and diagnosis of FCV infection and a gene fragment useful for preparation of said antibody are provided. Cell line 1D7 capable of producing a mouse monoclonal antibody having an excellent FCV-neutralizing activity was constructed and a gene fragment coding for the V region in charge of the FCV-specific binding of said antibody was obtained. This gene fragment and the gene coding for the constant region of the feline antibody are used to give a chimeric anti-FCV recombinant antibody. The obtained recombinant antibody is a novel antibody and is useful for the diagnosis, treatment and prevention of feline virus infections, particularly feline calicivirus infection, with high safety in administration into cats.Type: GrantFiled: March 1, 1993Date of Patent: July 28, 1998Assignee: Juridical Foundation The Chemo-Sero-Therapeutic Research InstituteInventors: Kazuhiko Kimachi, Hiroaki Maeda, Kiyoto Nishiyama, Sachio Tokiyoshi, Yukinobu Tohya, Takeshi Mikami
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Patent number: 5760185Abstract: An anti-FHV-1 recombinant antibody efficacious for treatment, prevention and diagnosis of feline herpes virus-1 (FHV-1) and a gene fragment useful for preparing the same are provided. A cell producing a mouse monoclonal antibody having an excellent neutralizing activity against FHV-1 was constructed, and a gene fragment coding for V region of said antibody responsible for the specific binding activity against FHV-1 was obtained. Using this gene fragment and a gene fragment coding for the constant region of a feline antibody, a chimeric anti-FHV-1 recombinant antibody is obtained.Type: GrantFiled: May 26, 1995Date of Patent: June 2, 1998Assignee: Juridical Foundation The Chemo-Sero-Therapeutic Research InstituteInventors: Kazuhiko Kimachi, Hiroaki Maeda, Kiyoto Nishiyama, Sachio Tokiyoshi
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Patent number: 5683891Abstract: A novel method for production of an exogenous protein is provided, the method being suitable for expression of an exogenous protein, especially a recombinant antibody etc., in an eucaryotic cell by utilizing a genetic recombination technique. That is, a novel method for production of an exogenous protein is provided which allows for culture of a cell in which an exogenous gene is introduced in a serum-free medium and an efficient production of an exogenous protein, by using, as a host cell for expression, a fused cell which is prepared by fusing a mouse myeloma and a lymphatic cell and which can be cultured in a serum-free medium.Type: GrantFiled: May 26, 1995Date of Patent: November 4, 1997Assignee: Juridical Foundation The Chemo-Sero Therapeutic Research InstituteInventors: Kiyoto Nishiyama, Yuji Ishikawa, Kazuhiko Kimachi, Hiroaki Maeda, Sachio Tokiyoshi
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Patent number: 4883752Abstract: Disclosed is a monoclonal antibody to HBsAg which is prepared by forming hybridomas between human peripheral blood lymphocyte cells, derived from humans having high titers of anti-HBsAg, and myeloma cells, cloning the hybridomas and selecting the antibody-producing clones. The monoclonal antibody can react with all the subtypes of HBsAg and is expected to be very effective in diagnosis and treatment of diseases due to the viral infection.Type: GrantFiled: October 24, 1985Date of Patent: November 28, 1989Assignee: Juridical Foundation the Chemo-Sero-Therapeutic Research InstituteInventors: Yasuyuki Eda, Toshihiro Maeda, Kiyoto Nishiyama, Akira Tashiro