Patents by Inventor Kiyoyuki Matsumura
Kiyoyuki Matsumura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10294465Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: GrantFiled: September 26, 2018Date of Patent: May 21, 2019Assignee: TAKARA BIO INC.Inventors: Kiyoyuki Matsumura, Nariaki Takatsu, Takashi Uemori, Hiroyuki Mukai
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Patent number: 10280412Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: GrantFiled: December 12, 2018Date of Patent: May 7, 2019Assignee: TAKARA BIO INC.Inventors: Kiyoyuki Matsumura, Nariaki Takatsu, Takashi Uemori, Hiroyuki Mukai
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Publication number: 20190100736Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: ApplicationFiled: December 12, 2018Publication date: April 4, 2019Applicant: TAKARA BIO INC.Inventors: Kiyoyuki MATSUMURA, Nariaki TAKATSU, Takashi UEMORI, Hiroyuki MUKAI
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Publication number: 20190085307Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: ApplicationFiled: September 26, 2018Publication date: March 21, 2019Applicant: TAKARA BIO INC.Inventors: Kiyoyuki MATSUMURA, Nariaki TAKATSU, Takashi UEMORI, Hiroyuki MUKAI
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Patent number: 10196618Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: GrantFiled: September 26, 2018Date of Patent: February 5, 2019Assignee: TAKARA BIO INC.Inventors: Kiyoyuki Matsumura, Nariaki Takatsu, Takashi Uemori, Hiroyuki Mukai
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Publication number: 20190017037Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: ApplicationFiled: September 26, 2018Publication date: January 17, 2019Applicant: TAKARA BIO INC.Inventors: Kiyoyuki MATSUMURA, Nariaki TAKATSU, Takashi UEMORI, Hiroyuki MUKAI
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Patent number: 10131890Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: GrantFiled: March 13, 2014Date of Patent: November 20, 2018Assignee: TAKARA BIO INC.Inventors: Kiyoyuki Matsumura, Nariaki Takatsu, Takashi Uemori, Hiroyuki Mukai
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Patent number: 10093968Abstract: Provided are the following: a method, for improving reactivity of an acid synthesis reaction, comprising a step for adding an ?-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleoside triphosphate, and an ?-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ?-amino acid.Type: GrantFiled: May 23, 2017Date of Patent: October 9, 2018Assignee: TAKARA BIO INC.Inventors: Kiyoyuki Matsumura, Takashi Uemori, Hiroyuki Mukai
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Publication number: 20170260578Abstract: Provided are the following: a method, for improving reactivity of an acid synthesis reaction, comprising a step for adding an ?-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleoside triphosphate, and an ?-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ?-amino acid.Type: ApplicationFiled: May 23, 2017Publication date: September 14, 2017Applicant: TAKARA BIO INC.Inventors: Kiyoyuki MATSUMURA, Takashi UEMORI, Hiroyuki MUKAI
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Patent number: 9689013Abstract: Provided are the following: a method, for improving reactivity of a nucleic acid synthesis reaction, comprising a step for adding an ?-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleotide triphosphate, and an ?-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ?-amino acid.Type: GrantFiled: January 24, 2013Date of Patent: June 27, 2017Assignee: TAKARA BIO INC.Inventors: Kiyoyuki Matsumura, Takashi Uemori, Hiroyuki Mukai
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Publication number: 20160017300Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.Type: ApplicationFiled: March 13, 2014Publication date: January 21, 2016Applicant: TAKARA BIO INC.Inventors: Kiyoyuki MATSUMURA, Nariaki TAKATSU, Takashi UEMORI, Hiroyuki MUKAI
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Publication number: 20140363849Abstract: Provided are the following: a method, for improving reactivity of a nucleic acid synthesis reaction, comprising a step for adding an ?-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleotide triphosphate, and an ?-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ?-amino acid.Type: ApplicationFiled: January 24, 2013Publication date: December 11, 2014Applicant: Takara Bio Inc.Inventors: Kiyoyuki Matsumura, Takashi Uemori, Hiroyuki Mukai
