Abstract: A system and a methodology are provided with a broad range of application in immune diagnostic screening and therapy and specifically for predicting the risk of graft versus host disease (GVHD) and/or graft versus leukemia (GVL) effects prior to transplantation. The method includes the steps of incubating single T or NK cells with a few, usually three to five target cells for extended period of times and evaluating the cell contact-dependent lytic activity or activation of the single effector cells within larger populations. The results obtained are analyzed by proprietary software for automated image analysis and compared with patient data comprised in a comprehensive database containing accumulated empirical and clinical information on donor-recipient screening results and patient information.

Abstract: The following observation shows that antigens or epitopes associated with impaired cellular peptide processing, especially MHC class I dependent, especially antigens associated with impaired TAP-function, can be used for immunization against cancer or as a virus vaccine.