Patents by Inventor Klaus Breddam
Klaus Breddam has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20190078042Abstract: According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which—through further enzymatic or spontaneous reactions—may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off-flavors, even after prolonged storage of the beverage.Type: ApplicationFiled: September 28, 2018Publication date: March 14, 2019Applicant: CARLSBERG A/SInventors: Klaus BREDDAM, Ole OLSEN, Birgitte SKADHAUGE, Finn LOK, Soren KNUDSEN, Lene Molskov BECH
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Patent number: 9834760Abstract: According to the invention, there is provided barley-derived beverages characterized by notably reduced levels of both dimethyl sulfide (DMS) and/or its precursor S-methyl-L-methionine (SMM), or lacking said compounds. In addition, the invention relates to methods for producing the above-mentioned beverages—and also to barley plants useful in the preparation of such beverages, as well as other plant products prepared from said plants. Utilization of the invention clears the way for making improved production procedures of beverages with improved taste profiles, and promises also for notable reductions in the thermal energy input for production of beer.Type: GrantFiled: December 1, 2009Date of Patent: December 5, 2017Assignees: CARLSBERG BREWERIES A/S, HEINEKEN SUPPLY CHAIN B.V.Inventors: Søren Knudsen, Gustav Hambraeus, Lene Molskov Bech, Steen Bech Sørensen, Birgitte Skadhauge, Klaus Breddam, Ole Olsen
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Patent number: 9363959Abstract: According to the invention, there is provided barley with total loss of functional lipoxygenase (LOX)-1 and LOX-2 enzymes, and plant products produced thereof, such as malt manufactured by using barley kernels defective in the synthesis of the fatty acid-dioxygenating enzymes LOX-1 and LOX-2. Said enzymes account for the principal activities related to dioxygenation of linoleic acid into 9- and 13-hydroperoxy octadecadienoic acid, respectively. 9-Hydroperoxy octadecadienoic acid represents a LOX pathway metabolite, which—through further enzymatic or spontaneous reactions—may lead to the appearance of trans-2-nonenal (T2N). The invention enables brewers to produce a beer having insignificant levels of stale, T2N-specific off-flavors, even after prolonged storage of the beverage.Type: GrantFiled: December 28, 2009Date of Patent: June 14, 2016Assignees: CARLSBERG BREWERIES A/S, HEINEKEN SUPPLY CHAIN B.V.Inventors: Birgitte Skadhauge, Finn Lok, Klaus Breddam, Ole Olsen, Lette Mølskov Bech, Søren Knudsen
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Publication number: 20150218498Abstract: According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which-through further enzymatic or spontaneous reactions-may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off flavors, even after prolonged storage of the beverage.Type: ApplicationFiled: May 16, 2014Publication date: August 6, 2015Applicant: CARLSBERG A/SInventors: Klaus BREDDAM, Ole OLSEN, Birgitte SKADHAUGE, Finn LOK, Soren KNUDSEN, Lene Molskov BECH
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Publication number: 20110318469Abstract: According to the invention, there is provided barley with total loss of functional lipoxygenase (LOX)-1 and LOX-2 enzymes, and plant products produced thereof, such as malt manufactured by using barley kernels defective in the synthesis of the fatty acid-dioxygenating enzymes LOX-1 and LOX-2. Said enzymes account for the principal activities related to dioxygenation of linoleic acid into 9- and 13-hydroperoxy octadecadienoic acid, respectively. 9-Hydroperoxy octadecadienoic acid represents a LOX pathway metabolite, which—through further enzymatic or spontaneous reactions—may lead to the appearance of trans-2-nonenal (T2N). The invention enables brewers to produce a beer having insignificant levels of stale, T2N-specific off-flavors, even after prolonged storage of the beverage.Type: ApplicationFiled: December 28, 2009Publication date: December 29, 2011Applicants: Heineken Supply Chain B.V., Carlsberg Breweries A//SInventors: Birgitte Skadhauge, Finn Lok, Klaus Breddam, Ole Olsen, Lene Molskov Bech, Soren Knudsen
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Publication number: 20110293779Abstract: According to the invention, there is provided barley-derived beverages characterized by notably reduced levels of both dimethyl sulfide (DMS) and/or its precursor S-methyl-L-methionine (SMM), or lacking said compounds. In addition, the invention relates to methods for producing the above-mentioned beverages—and also to barley plants useful in the preparation of such beverages, as well as other plant products prepared from said plants. Utilization of the invention clears the way for making improved production procedures of beverages with improved taste profiles, and promises also for notable reductions in the thermal energy input for production of beer.Type: ApplicationFiled: December 1, 2009Publication date: December 1, 2011Applicants: Heineken Supply Chain B.V., Carlsberg Breweries A/SInventors: Søren Knudsen, Gustav Hambraeus, Lene Molskov Bech, Steen Bech Sorensen, Birgitte Skadhauge, Klaus Breddam, Ole Olsen
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Publication number: 20110195151Abstract: According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which-through further enzymatic or spontaneous reactions-may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off flavors, even after prolonged storage of the beverage.Type: ApplicationFiled: November 22, 2010Publication date: August 11, 2011Applicant: Carlsberg A/SInventors: Klaus Breddam, Ole Olsen, Birgitte Skadhauge, Finn Lok, Søren Knudsen, Lene Mølskov Bech
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Patent number: 7838053Abstract: According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which-through further enzymatic or spontaneous reactions-may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off flavors, even after prolonged storage of the beverage.Type: GrantFiled: March 9, 2005Date of Patent: November 23, 2010Assignee: Carlsberg A/SInventors: Klaus Breddam, Ole Olsen, Birgitte Skadhauge, Finn Lok, Søren Knudsen, Lene Mølskov Bech
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Publication number: 20090029000Abstract: According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which-through further enzymatic or spontaneous reactions may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off flavors, even after prolonged storage of the beverage.Type: ApplicationFiled: March 9, 2005Publication date: January 29, 2009Applicant: Carlsberg A/SInventors: Klaus Breddam, Ole Olsen, Birgitte Skadhauge, Finn Lok, Soren Knudsen, Lene Molskov Bech
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Patent number: 7420105Abstract: According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which—through further enzymatic or spontaneous reactions—may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off-flavors, even after prolonged storage of the beverage.Type: GrantFiled: March 11, 2004Date of Patent: September 2, 2008Assignee: Carlsberg A/SInventors: Klaus Breddam, Ole Olsen, Birgitte Skadhauge, Finn Lok, Soren Knudsen, Lene Molskov Bech
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Publication number: 20050204437Abstract: According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which—through further enzymatic or spontaneous reactions—may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off-flavors, even after prolonged storage of the beverage.Type: ApplicationFiled: March 11, 2004Publication date: September 15, 2005Applicant: CARLSBERG A/SInventors: Klaus Breddam, Ole Olsen, Birgitte Skadhauge, Finn Lok, Soren Knudsen, Lene Bech
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Publication number: 20010010923Abstract: A method for transamidating a peptide substrate having a P1 amino acid residue with a positively charged side chain. According to the invention, carboxypeptidase Y is modified to substitute at least one amino acid having a negatively charged side chain in an S1 subsite. Additionally, the modified carboxypeptidase Y can include substituted amino acid residues in an S1′, S2 and/or S3 subsite to accommodate a specific peptide substrate.Type: ApplicationFiled: October 19, 1999Publication date: August 2, 2001Inventors: UFFE MORTENSEN, KJELD OLESEN, HENNING STENNICKE, STEEN B. SORENSEN, KLAUS BREDDAM
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Patent number: 6187579Abstract: The invention provides customized proteases (i.e., mutant enzymes), methods of making customized proteases, as well as methods of using customized proteases. The customized proteases of the invention are derived from the known proteases. Altered transacylation reactions include the capability to perform transacylation reactions not substantially catalyzed by the known protease or the capability to perform transacylation reactions with improved yields, or both. The methods of the invention provide for customized proteases through site specific or random mutagenesis of the active site amino acids of the known proteases. The invention also provides for methods of using the customized proteases to prepare a preselected transacylation products. The preselected transacylation products produced can be modified by substitution at the N-or C-terminal with nucleophiles such as L-amino acids, D-amino acids, amino acid amides, and radioactive amino acids.Type: GrantFiled: October 27, 1994Date of Patent: February 13, 2001Assignee: Carlsberg A/SInventors: Klaus Breddam, Morten C. Kielland-Brandt, Uffe Hasbo Mortensen, Kjeld Ove Olesen, Henning Ralf Stennicke, Fred W. Wagner
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Patent number: 5993865Abstract: The present invention concerns a beverage containing proteins and/or peptides and is characterized in that it contains Cereal-LT and/or homologues as herein defined and/or a modified Cereal-LTP fraction obtainable from the Cereal-LTP and/or homologues by heating, boiling and/or mashing the Cereal-LTP and/or homologues in water at a pH between 3 and 7. The invention also concerns a method for preparing it and a use of a foam-forming additive.Type: GrantFiled: June 26, 1996Date of Patent: November 30, 1999Assignee: Carlsberg A/SInventors: Lene M.o slashed.lskov Bech, Steen Bech S.o slashed.rensen, Pia Vaag, Marianne Muldbjerg, Thorkild Beenfeldt, Robert Leah, Klaus Breddam
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Patent number: 5985627Abstract: A method for transamidating a peptide substrate having a P.sub.1 amino acid residue with a positively charged side chain. According to the invention, carboxypeptidase Y is modified to substitute at least one amino acid having a negatively charged side chain in an S.sub.1 subsite. Additionally, the modified carboxypeptidase Y can include substituted amino acid residues in an S.sub.1 ', S.sub.2 and/or S.sub.3 subsite to accommodate a specific peptide substrate.Type: GrantFiled: February 28, 1997Date of Patent: November 16, 1999Assignee: Carlsberg LaboratoryInventors: Uffe Mortensen, Kjeld Olesen, Henning Stennicke, Steen B. S.o slashed.rensen, Klaus Breddam
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Patent number: 5945329Abstract: The invention provides customized proteases (i.e., mutant enzymes), methods of making customized proteases, as well as methods of using customized proteases. The customized proteases of the invention are derived from the known proteases. Altered transacylation reactions include the capability to perform transacylation reactions not substantially catalyzed by the known protease or the capability to perform transacylation reactions with improved yields, or both. The methods of the invention provide for customized proteases through site specific or random mutagenesis of the active site amino acids of the known proteases. The invention also provides for methods of using the customized proteases to prepare a preselected transacylation products. The preselected transacylation products produced can be modified by substitution at the N- or C-terminal with nucleophiles such as L-amino acids, D-amino acids, amino acid amides, and radioactive amino acids.Type: GrantFiled: July 23, 1997Date of Patent: August 31, 1999Assignee: Carlsberg A/SInventors: Klaus Breddam, Morten C. Kielland-Brandt, Uffe Hasbo Mortensen, Kjeld Ove Olesen, Henning Ralf Stennicke, Fred W. Wagner
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Patent number: 5580751Abstract: A process for preparing C-terminally amidated peptides, Peptide-NH.sub.2, is presented. In a first step, a substrate component is reacted with a nucleophile component in the presence of trypsin or a carboxypeptidase using as nucleophile a compound NH.sub.2 -R to form a first reaction product Peptide-NH-R. In a second step, the first reaction product is non-enzymatically chemically cleaved to form the C-terminally amidated product, Peptide-NH.sub.2. The substrate component is selected from a) peptide derivatives Peptide-X-Y, where X is an amino acid or peptide residue and Y is OH, OMe or C-terminal modification and c) C-terminally esterified peptides, Peptide-OR', where R' is alkyl, aryl, heteroaryl, or aralkyl. The nucleophile component is selected from ##STR1## wherein A-F and A'-E' are carbon atoms or up to two hetero atoms, Y is H, alkyl, aryl, aralkyl, oxo or carboxy, X.sup.1 -X.sup.5 are H or various substituents.Type: GrantFiled: April 21, 1995Date of Patent: December 3, 1996Assignee: Carlsberg A/SInventors: Ole Buchardt, Klaus Breddam, Dennis Henriksen
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Patent number: 5234820Abstract: An auxiliary substance such as a label, support, or bioactive agent is attached to a protein at a site that is remote from the active site of the protein by the use of exopeptidase and a nucleophile which is an amino acid, amino acid derivative, amine or alcohol. In one embodiment, the nucleophile is attached to the carboxy terminus of a protein by catalysis with exopeptidase to form an adduct and then the adduct or its combination with a linker arm is bound to the auxiliary substance. In another embodiment, the auxiliary substance or its combination with a linker arm is bound to the nucleophile to form an intermediate substance which is then coupled by catalysis with exopeptidase to the carboxy terminus of a protein.Type: GrantFiled: June 28, 1990Date of Patent: August 10, 1993Assignees: Board of Regents of the University of Nebraska, BioNebraska, Inc.Inventors: Fred W. Wagner, Thomas R. Coolidge, Sheldon M. Schuster, Jay Stout, Dwane E. Wylie, Klaus Breddam, William Lewis
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Patent number: 5208158Abstract: Novel chemically modified detergent enzymes are provided, wherein one or more methionines have been mutated into cysteines, said cysteines subsequently being chemically modified in order to confer the enzyme improved stability towards oxidative agents. A novel process for stabilizing detergent enzymes against oxidation is also provided. Furthermore, there are provided detergent compositions comprising these novel oxidation stable detergent enzymes.Type: GrantFiled: July 17, 1990Date of Patent: May 4, 1993Assignee: Novo Nordisk A/SInventors: Lene M. Bech, Sven Branner, Klaus Breddam, Hanne Groen
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Patent number: 4645740Abstract: The B-30 amino acid in insulins is replaced enzymatically byreacting as substrate component the selected insulin Ins-X, wherein X represents the B-30 amino acidwith an amine component selected from the group consisting of(a) amino acids of the formulaH--B--OHwherein B is an amino acid residue,(b) optionally N-substituted amino acid amides of the formulaH--B--NR.sup.1 R.sup.2wherein B is an amino acid residue and R.sup.1 and R.sup.2 are independently selected from the group consisting of hydrogen, amino, hydroxy, alkyl, cycloalkyl, aryl, heteroaryl and aralkyl or R.sup.1 and R.sup.2 together with the nitrogen atom form a heterocyclic group which may contain a further hetero atom, and(c) amino acid esters of the formulaH--B--OR.sup.3, H--B--SR.sup.3 or H--B--SeR.sup.3wherein B is am amino acid residue and R.sup.Type: GrantFiled: March 23, 1982Date of Patent: February 24, 1987Assignee: Carlsberg Biotechnology Ltd. A/SInventors: Klaus Breddam, Jack T. Johansen, Fred Widmer