Abstract: The invention relates to the pseudotyping of retroviral vectors with heterologous envelope proteins derived from the Paramyxoviridae family, genus Morbillivirus, and various uses of the resulting vector particles. The present invention is based on the unexpected and surprising finding that the incorporation of morbillivirus F and H proteins having truncated cytoplasmic tails into lentiviral vector particles, and the complex interaction of these two proteins during cellular fusion, allows for a superior and more effective transduction of cells. Moreover, these pseudotyped vector particles allow the targeted gene transfer into a given cell type of interest by modifying a mutated and truncated H protein with a single-chain antibody or ligand directed against a cell surface marker of the target cell.

Abstract: The invention relates to the pseudotyping of retroviral vectors with heterologous envelope proteins derived from the Paramyxoviridae family, genus Morbillivirus, and various uses of the resulting vector particles. The present invention is based on the unexpected and surprising finding that the incorporation of morbillivirus F and H proteins having truncated cytoplasmic tails into lentiviral vector particles, and the complex interaction of these two proteins during cellular fusion, allows for a superior and more effective transduction of cells. Moreover, these pseudotyped vector particles allow the targeted gene transfer into a given cell type of interest by modifying a mutated and truncated H protein with a single-chain antibody or ligand directed against a cell surface marker of the target cell.

Abstract: The invention relates to the pseudotyping of retroviral vectors with heterologous envelope proteins derived from the Paramyxoviridae family, genus Morbillivirus, and various uses of the resulting vector particles. The present invention is based on the unexpected and surprising finding that the incorporation of morbillivirus F and H proteins having truncated cytoplasmic tails into lentiviral vector particles, and the complex interaction of these two proteins during cellular fusion, allows for a superior and more effective transduction of cells. Moreover, these pseudotyped vector particles allow the targeted gene transfer into a given cell type of interest by modifying a mutated and truncated H protein with a single-chain antibody or ligand directed against a cell surface marker of the target cell.

Abstract: The invention relates to APOBEC4 proteins and N- or C-terminally modified APOBEC4 proteins for the modulation of the release of retroviruses or viral particles thereof from cells and various uses thereof. Expression of APOBEC4 or N-terminally stabilized APOBEC4 protein in a cell leads to an increased viral production, while expression of C-terminally modified APOBEC4 protein leads to a decrease in viral production.

Abstract: The invention relates to the pseudotyping of retroviral vectors with heterologous envelope proteins derived from the Paramyxoviridae family, genus Morbillivirus, and various uses of the resulting vector particles. The present invention is based on the unexpected and surprising finding that the incorporation of morbillivirus F and H proteins having truncated cytoplasmic tails into lentiviral vector particles, and the complex interaction of these two proteins during cellular fusion, allows for a superior and more effective transduction of cells. Moreover, these pseudotyped vector particles allow the targeted gene transfer into a given cell type of interest by modifying a mutated and truncated H protein with a single-chain antibody or ligand directed against a cell surface marker of the target cell.

Abstract: The invention relates to retroviral vectors (known as lentiviral vectors), which are used to transfer genes into cells that are at cell cycle stage G0, to methods for their production and to the use thereof for transferring genes into mammalian cells. Said vectors are derived from SIVsmmPBj14 (simian immunodeficiency virus) of the sooty mangabey monkey, strain PBj 14.

Abstract: The invention relates to cell-specific retroviral vectors with antibody recognition domains (scFv), which are suitable for cell-specific transduction of a selected mammal cell type (cell targeting), to methods for the production of the cell-specific retroviral vectors and to the use thereof in gene transfers into selected cells. The invention further relates to retroviral packaging cells to obtain cell-specific vectors according to the present invention.

Abstract: The invention relates to gene transfer into human T cells using novel retroviral scFv cell targeting vectors and using these vectors for the treatment of T-cell-associated diseases.

Abstract: The invention relates to retroviral vectors (known as lentiviral vectors), which are used to transfer genes into cells that are at cell cycle stage G0, to methods for their production and to the use thereof for transferring genes into mammalian cells. Said vectors are derived from SIVsmmPBj14 (simian immunodeficiency virus) of the sooty mangabey monkey, strain PBj 14.

Abstract: The invention relates to cell-specific retroviral vectors with antibody recognition domains (scFv), which are suitable for cell-specific transduction of a selected mammal cell type (cell targeting), to methods for the production of the cell-specific retroviral vectors and to the use thereof in gene transfers into selected cells. The invention further relates to retroviral packaging cells to obtain cell-specific vectors according to the present invention.

Abstract: The invention relates to the production and use of retroviral vectors for cell specific gene transfer, specially to a production method of retroviral vectors containing capsid particles of murine leukemia virus (MLV) and envelope proteins of human immunodeficiency vises (HIV) or simian immunodeficiency viruses (SIV). Said vectors can be used for gene transfer in selected cell types, specially in CD4-positive mammal cells.

Abstract: The invention relates to pseudo-typed retroviral vectors having modified surface capsid proteins suitable for cell-specific transduction of a selected mammalian cell type (cell targeting), methods for the preparation of the cell-specific pseudo-typed retroviral vectors and to their use in gene transfer into selected cells.

Abstract: The preparation and use of novel lentiviral SiVagm-derived vectors for gene transfer and in particular a method of preparation of the lentiviral vectors containing capsid particles of the simian immunodeficiency virus SIVagm and envelope proteins of SIVagm and other retroviruses such as the human immunodeficiency viruses (HIV), other simian immunodeficiency viruses (SIV), other retroviruses such as the murine leukemia virus (MLV) or the “gibbon ape leukemia virus” (GaLV) or the porcine endogenous or exogenous retrovirus (PERV), the “vesicular stomatitis virus” (VSV-G) are described. The vectors and corresponding packaging cells may be employed for packaging and transfer of genes which are not packaged by other lentiviral, retroviral and other vectors or which show an inefficient gene transfer with other vector particles. These vectors may be used for gene transfer into selected cell types, specifically into proliferatively active and resting human cells.

Abstract: The preparation and use of novel lentiviral SIVagm-derived vectors for gene transfer and in particular a method of preparation of the lentiviral vectors containing capsid particles of the simian immunodeficiency virus SIVagm and envelope proteins of SIVagm and other retroviruses such as the human immunodeficiency viruses (HIV), other simian immunodeficiency viruses (SIV), other retroviruses such as the murine leukemia virus (MLV) or the “gibbon ape leukemia virus” (GaLV) or the porcine endogenous or exogenous retrovirus (PERV), the “vesicular stomatitis virus” (VSV-G) are described. The vectors and corresponding packaging cells may be employed for packaging and transfer of genes which are not packaged by other lentiviral, retroviral and other vectors or which show an inefficient gene transfer with other vector particles. These vectors may be used for gene transfer into selected cell types, specifically into proliferatively active and resting human cells.