Abstract: The present invention relates to composite materials useful for purifying proteins obtained from biological feedstocks. The composite materials of the invention comprise a porous support having an average pore size of 5 to 500 nm, said porous support being filled with a first polymer which is cross-linked, and a second polymer which is not crosslinked and is covalently bonded to the external surface of the cross-linked polymer-filled porous support.

Abstract: Filter media, filter elements, and arrangements of filters, wherein at least one polymeric mesh adsorbent is comprising at least one functional polymer or derivative of a functional polymer, capable of binding contaminants from a gas mixture, preferably proteins, peptides, glycoproteins, lipoproteins, nucleic acids, carbohydrates, and lipids. These contaminants may exhibit allergenic or toxic properties. These contaminants are preferably embedded in aerosols or attached to small particles. Processes for the synthesis of a polymeric mesh, whereas at least one functional polymer is immobilized via generation of amide or ester bonds, whereas all reactants are not activated and not comprising active groups.

Abstract: Method for recovering a target protein from a feedstock comprising said target protein and at least one impurity compound selected from host cell proteins (HCP), DNA, RNA or other nucleic acid, the target protein being characterized by a hydrodynamic radius Rh1 and the impurity compound being characterized by a hydrodynamic radius Rh2, wherein Rh1>Rh2, comprising the following steps (i) to (iv) and optionally step (v): (i) providing a polymeric mesh comprising at least one crosslinked polymer containing positively charged amino groups, wherein the polymer has a pore size exclusion limit Rhi which can be set variably; (ii) adapting the variable pore size exclusion limit Rhi of the polymeric mesh such that Rh2<Rhi and Rh1>Rhi; (iii) contacting the polymeric mesh with the feedstock; (iv) separating the polymeric mesh containing the retained impurity compound from the feedstock containing the excluded target protein.

Abstract: Composite material comprising a support material and at least one polymeric layer, wherein the at least one polymeric layer is present in form of a polymeric mesh and is comprising at least one non-adsorbing/non-adsorptive polymer with respect to a target compound, and wherein said composite material further comprises sites which are adsorbing/adsorptive for an impurity compound; and a combination of at least one first adsorbent and at least one second adsorbent, wherein the at least one first adsorbent comprises at least one composite material comprising at least one adsorbing/adsorptive polymer, or at least one non-adsorbing/non-adsorptive polymer, or at least one adsorbing/adsorptive polymer together with at least one non-adsorbing/non-adsorptive polymer.

Abstract: Method for recovering a target protein from a feedstock comprising said target protein and at least one impurity compound selected from host cell proteins (HCP), DNA, RNA or other nucleic acid, the target protein being characterized by a hydrodynamic radius Rh1 and the impurity compound being characterized by a hydrodynamic radius Rh2, wherein Rh1>Rh2, comprising the following steps (i) to (iv) and optionally step (v): (i) providing a polymeric mesh comprising at least one crosslinked polymer containing positively charged amino groups, wherein the polymer has a pore size exclusion limit Rhi which can be set variably; (ii) adapting the variable pore size exclusion limit Rhi of the polymeric mesh such that Rh2<Rhi and Rh1>Rhi; (iii) contacting the polymeric mesh with the feedstock; (iv) separating the polymeric mesh containing the retained impurity compound from the feedstock containing the excluded target protein.

Abstract: Sorbent comprising a solid support material, the surface of which comprises first residues comprising a binuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S, and second residues comprising a mononuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S.

Abstract: Sorbent comprising a solid support material, the surface of which comprises a first residue comprising a pyridyl ring, whose hydrogen atoms may be substituted, and a second residue comprising a carboxyl group. The first and second residues may be attached to a polymer which coats a carrier, but which polymer is not covalently grafted to the carrier. The sorbents can be used to separate or to increase the concentration and/or purity of a protein or peptide from a mixture containing the same.

Abstract: Sorbent comprising a solid support material, the surface of which comprises first residues comprising a binuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S, and second residues comprising a mononuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S.

Abstract: Sorbent comprising a solid support material, the surface of which comprises a first residue comprising a pyridyl ring, whose hydrogen atoms may be substituted, and a second residue comprising a carboxyl group.

Abstract: A method for the manufacture of at least one sorbent having at least two different groups, which are capable of binding, for the selective binding of a substrate, characterized in that it comprises the steps (i) to (ii): (i) determining at least two groups capable of binding a sorbent from a synthetic or natural first substrate, (ii) respectively applying at least two different groups capable of binding a second synthetic or natural substrate to one respective carrier, thereby forming at least one sorbent, whereby the groups are the same groups of step (i) or are groups that are complementary thereto, and the second substrate of step (ii) is the same or different from the first substrate according to step (i), and whereby the groups are determined such that the contributions of the Gibbs energies of the individual groups to the non-covalent bond with the second substrate yield a negative value of the Gibbs energy ?G, such that a binding strengthening occurs that results in an improved separation selectivity

Abstract: In a process, a polymer is crosslinked and then reversibly bound to a template that is dissolved or suspended in a solvent. The polymer thereby acquires a conformation adapted to the template such that an interaction enthalpy between the template and the polymer is increased by more than 0.1 kcal/mole. The template can be a chemical compound or a biological structure.

Abstract: In a process, a polymer is crosslinked and then reversibly bound to a template that is dissolved or suspended in a solvent. The polymer thereby acquires a conformation adapted to the template such that an interaction enthalpy between the template and the polymer is increased by more than 0.1 kcal/mole. The template can be a chemical compound or a biological structure.

Abstract: Process for the preparation of a polymeric network, in which (i) one or more polymers are made available which can be crosslinked with one another intramolecularly or intermolecularly or intra- and intermolecularly by covalent or non-covalent bonding, (ii) the conformation of at least one of the polymers is adapted to at least one template compound with obtainment of at least one preferred conformation of the at least one polymer, (iii) at least one of the preferred conformations obtained according to (ii) is fixed by crosslinkage.

Abstract: A method for the manufacture of at least one sorbent having at least two different groups, which are capable of binding, for the selective binding of a substrate, characterized in that it comprises the steps (i) to (ii): (i) determining at least two groups capable of binding a sorbent from a synthetic or natural first substrate, (ii) respectivly applying at least two different groups capable of binding a second synthetic or natural substrate to one respective carrier, thereby forming at least one sorbent, whereby the groups are the same groups of step (i) or are groups that are complementary thereto, and the second substrate of step (ii) is the same or different from the first substrate according to step (i), and whereby the groups are determined such that the contributions of the Gibbs energies of the individual groups to the non-covalent bond with the second substrate yield a negative value of the Gibbs energy ?G, such that a binding strengthening occurs that results in an improved separation selectivity wi

Abstract: Process for the preparation of a derivative of a polymer having at least one functional group, characterized in that the process comprises the following step (i): (i) reaction of the polymer having at least one functional group, with at least one activating reagent or at least one derivative of an activating reagent in homogeneous phase.

Abstract: Process for the preparation of a condensation compound by reaction of at least one functional group of a first low-molecular weight compound having at least two functional groups, with at least one functional group of at least one further, second low-molecular weight compound having at least two functional groups, and which can be identical to the first or different from the first low-molecular weight compound, with obtainment of a condensation compound, characterized in that at least one of the functional groups involved in this reaction has been activated before the reaction by reaction with a compound of the following structure (I) where R′ is a halogen atom or a radical (I′) and where R1, R2, R1′ and R2′ are identical or different and are hydrogen, straight-chain or branched-chain alkyl, aryl, cycloalkyl, heterocyclic or aralkyl radicals having up to 30 C atoms, or either R1 and R2 or R1′ and R2′ or both R1 and R2 and R1′ and R2