Patents by Inventor Klaus Gregorius
Klaus Gregorius has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11879027Abstract: Disclosed is agents and methods that target metabolism malfunctions, inborne as well as acquired, as well as methods for preparation of the agents. In particular, the invention relates to methods for preparing molecular imprinted polymers with high binding capacity for phenylalanine or tyrosine, MIPs that bind phenylalanine or tyrosine, and methods for treating phenylketonuria, alkaptonuria, and hypertyrosinemia.Type: GrantFiled: May 11, 2021Date of Patent: January 23, 2024Assignee: MIPSALUS APSInventors: Klaus Gregorius, Nicolas Otto Krogh
-
Patent number: 11549948Abstract: Disclosed is a FRET based assay and related products. The assay employs molecular imprinted polymers having a very high affinity for their target.Type: GrantFiled: March 15, 2017Date of Patent: January 10, 2023Assignee: MIPSALUS APSInventors: Nicolas Otto Krogh, Klaus Gregorius, Elena Fernandez Laborda
-
Publication number: 20210277158Abstract: Disclosed is agents and methods that target metabolism malfunctions, inborne as well as acquired, as well as methods for preparation of the agents. In particular, the invention relates to methods for preparing molecular imprinted polymers with high binding capacity for phenylalanine or tyrosine, MIPs that bind phenylalanine or tyrosine, and methods for treating phenylketonuria, alkaptonuria, and hypertyrosinemia.Type: ApplicationFiled: May 11, 2021Publication date: September 9, 2021Inventors: Klaus Gregorius, Nicolas Otto Krogh
-
Patent number: 11028203Abstract: Disclosed is agents and methods that target metabolism malfunctions, inborne as well as acquired, as well as methods for preparation of the agents. In particular, the invention relates to methods for preparing molecular imprinted polymers with high binding capacity for phenylalanine or tyrosine, MIPs that bind phenylalanine or tyrosine, and methods for treating phenylketonuria, alkaptonuria, and hypertyrosinemia.Type: GrantFiled: June 29, 2018Date of Patent: June 8, 2021Assignee: Mipsalus APSInventors: Klaus Gregorius, Nicolas Otto Krogh
-
Publication number: 20200166516Abstract: Disclosed is a FRET based assay and related products. The assay employs molecular imprinted polymers having a very high affinity for their target.Type: ApplicationFiled: March 15, 2017Publication date: May 28, 2020Applicant: Mipsalus ApSInventors: Nicolas Otto Krogh, Klaus Gregorius, Elena Fernandez Laborda
-
Publication number: 20200140591Abstract: Disclosed is agents and methods that target metabolism malfunctions, inborne as well as acquired, as well as methods for preparation of the agents. In particular, the invention relates to methods for preparing molecular imprinted polymers with high binding capacity for phenylalanine or tyrosine, MIPs that bind phenylalanine or tyrosine, and methods for treating phenylketonuria, alkaptonuria, and hypertyrosinemia.Type: ApplicationFiled: June 29, 2018Publication date: May 7, 2020Inventors: Klaus Gregorius, Nicolas Otto Krogh
-
Publication number: 20150374742Abstract: Disclosed is a method for preparing a composition enriched for receptors (typically molecular impringet polymers, MIPs) that bind an agent, where said receptors each specifically bind at least two discrete sites on said agent, by subjecting a sample of receptors to a first step of affinity purification with the agent where one binding site on the agent is non-accessible for binding to the receptors and subsequently subjecting the purified receptors to at least one further step of affinity purification with the agent where a second binding site on the agent is non-accessible.Type: ApplicationFiled: September 9, 2015Publication date: December 31, 2015Inventors: Nicolas Otto Krogh, Klaus Gregorius
-
Patent number: 9212271Abstract: Provided is an improved method for preparation of insoluble molecular imprinted polymers (MIPs), the method comprising: a) providing soluble or semi-soluble MIPs that 1) substantially all bind template agents and 2) have sizes which enable their separation in a chromatographic step utilizing packed bed chromatography, b) cross-linking the template agent binding soluble MIPs provided in step a so as to obtain insoluble template agent binding MIPs, and c) optionally isolating, concentrating or purifying the MIPs obtained by the cross-linking in step b. In an interesting embodiment, step a includes an affinity purification procedure, which ensures that the MIPs provided in step a are indeed all binders of the template.Type: GrantFiled: February 28, 2012Date of Patent: December 15, 2015Assignee: Mipsalus APSInventors: Klaus Gregorius, Ian Alan Nicholls, Nicolas Otto Krogh
-
Patent number: 9149737Abstract: Disclosed is a method for preparing a composition enriched for receptors (typically molecular impringet polymers, MIPs) that bind an agent, where said receptors each specifically bind at least two discrete sites on said agent, by subjecting a sample of receptors to a first step of affinity purification with the agent where one binding site on the agent is non-accessible for binding to the receptors and subsequently subjecting the purified receptors to at least one further step of affinity purification with the agent where a second binding site on the agent is non-accessible.Type: GrantFiled: September 16, 2010Date of Patent: October 6, 2015Assignee: Mipsalus ApSInventors: Nicolas Otto Krogh, Klaus Gregorius
-
Publication number: 20150031779Abstract: Provided is an improved method for preparation of insoluble molecular imprinted polymers (MIPs), the method comprising: a) providing soluble or semi-soluble MIPs that 1) substantially all bind template agents and 2) have sizes which enable their separation in a chromatographic step utilizing packed bed chromatography, b) cross-linking the template agent binding soluble MIPs provided in step a so as to obtain insoluble template agent binding MIPs, and c) optionally isolating, concentrating or purifying the MIPs obtained by the cross-linking in step b. In an interesting embodiment, step a includes an affinity purification procedure, which ensures that the MIPs provided in step a are indeed all binders of the template.Type: ApplicationFiled: February 28, 2012Publication date: January 29, 2015Applicant: Mipsalus APSInventors: Klaus Gregorius, Ian Alan Nicholls, Nicolas Otto Krogh
-
Patent number: 8871212Abstract: Disclosed are novel methods and compositions for combating diseases characterized by deposition of amyloid. The methods generally rely on immunization against amyloid precursor protein (APP) or beta amyloid (A?). Immunization is preferably effected by administration of analogs of autologous APP or A?, said analogs being capable of inducing antibody production against the autologous amyloidogenic polypeptides. Especially preferred as an immunogen is autologous A? which has been modified by introduction of one single or a few foreign, immunodominant and promiscuous T-cell epitopes. Such methods and means include methods for the preparation of analogs and pharmaceutical formulations, as well as nucleic acid fragments, vectors, transformed cells, polypeptides and pharmaceutical formulations.Type: GrantFiled: December 19, 2008Date of Patent: October 28, 2014Assignee: H. Lundbeck A/SInventors: Peter Birk Rasmussen, Martin Roland Jensen, Klaus Gregorius Nielsen, Peter Koefoed, Florence Dal Degan
-
Publication number: 20140200336Abstract: A sensor for the detection or measurement of a carbohydrate analyte in fluid comprises components of a competitive binding assay the readout of which is a detectable or measurable optical signal retained by a material that permits diffusion of the analyte but not the assay components, the assay components comprising: a carbohydrate binding molecule labelled with one of a proximity based signal generating/modulating moiety pair; and a carbohydrate analogue capable of competing with the analyte for. binding to the carbohydrate binding molecule, the carbohydrate analogue being a flexible water-soluble polymer comprising: polymerized or co-polymerised residues of monomer units, the monomer unit residues bearing pendant carbohydrate or carbohydrate mimetic moieties and pendant moieties which are the other of the proximity based signal generating/modulating moiety pair.Type: ApplicationFiled: February 12, 2014Publication date: July 17, 2014Applicant: MEDTRONIC MINIMED, INC.Inventors: Jesper Svenning KRISTENSEN, Klaus GREGORIUS, Casper STRUVE, Yihua YU
-
Patent number: 8700113Abstract: A sensor for the detection or measurement of carbohydrate analyte (such as glucose) in fluid comprises components of a competitive binding assay the readout of which is a detectable or measurable optical signal (such as FRET assay) retained by a material that permits diffusion of analyte but not the assay components, the assay components comprising: an animal lectin; and an analyte analogue capable of competing with analyte for binding to the lectin.Type: GrantFiled: December 7, 2005Date of Patent: April 15, 2014Assignee: Medtronic Minimed, Inc.Inventors: Jesper Svenning Kristensen, Klaus Gregorius, Casper Struve, John Myhre Frederiksen, Yihua Yu
-
Patent number: 8691517Abstract: A sensor for the detection or measurement of a carbohydrate analyte in fluid comprises components of a competitive binding assay the readout of which is a detectable or measurable optical signal retained by a material that permits diffusion of the analyte but not the assay components, the assay components comprising: a carbohydrate binding molecule labelled with one of a proximity based signal generating/modulating moiety pair; and a carbohydrate analogue capable of competing with the analyte for binding to the carbohydrate binding molecule, the carbohydrate analogue being a flexible water-soluble polymer comprising: polymerized or co-polymerised residues of monomer units, the monomer unit residues bearing pendant carbohydrate or carbohydrate mimetic moieties and pendant moieties which are the other of the proximity based signal generating/modulating moiety pair.Type: GrantFiled: December 6, 2006Date of Patent: April 8, 2014Assignee: Medtronic Minimed, Inc.Inventors: Jesper Svenning Kristensen, Klaus Gregorius, Casper Struve, Yihua Yu
-
Patent number: 8478375Abstract: A sensor for sensing analyte concentration comprises at least two different variants of an appropriate competitive binding assay, the sensor being capable of sensing accurately a required range of analyte concentrations by means of the variants of the assay each being capable of sensing accurately a part only of the required range of analyte concentrations and the variants of the assay being chosen to sense overlapping or adjoining ranges of concentration covering the whole of the required range.Type: GrantFiled: December 7, 2005Date of Patent: July 2, 2013Assignee: Medtronic Minimed, Inc.Inventors: Casper Struve, Jesper Svenning Kristensen, Klaus Gregorius, Yihua Yu
-
Publication number: 20120171154Abstract: Disclosed is a method for preparing a composition enriched for receptors (typically molecular impringet polymers, MIPs) that bind an agent, where said receptors each specifically bind at least two discrete sites on said agent, by subjecting a sample of receptors to a first step of affinity purification with the agent where one binding site on the agent is non-accessible for binding to the receptors and subsequently subjecting the purified receptors to at least one further step of affinity purification with the agent where a second binding site on the agent is non-accessible.Type: ApplicationFiled: September 16, 2010Publication date: July 5, 2012Applicant: Mipsalus ApSInventors: Nicolas Otto Krogh, Klaus Gregorius
-
Patent number: 8022039Abstract: The present invention provides for a novel method for purification of EGFR family proteins obtained from cultures of insect cells. The process comprises subsequent steps of a) diafiltration and exchange of culture medium with buffer, b) immobilized metal affinity chromatography (IMAC), C) size exclusion chromatography (SEC), and d) anion exchange chromatography (AIE). The method also provides for an immunogenic variant of HER-2 protein which for which the purification process has been especially adapted, as well as means for the preparation of the variant.Type: GrantFiled: June 28, 2010Date of Patent: September 20, 2011Assignee: BN ImmunoTherapeutics, Inc.Inventors: Marie Eskling, Klaus Gregorius Nielsen
-
Publication number: 20100285071Abstract: The present invention provides for a novel method for purification of EGFR family proteins obtained from cultures of insect cells. The process comprises subsequent steps of a) diafiltration and exchange of culture medium with buffer, b) immobilized metal affinity chromatography (IMAC), C) size exclusion chromatography (SEC), and d) anion exchange chromatography (AIE). The method also provides for an immunogenic variant of HER-2 protein which for which the purification process has been especially adapted, as well as means for the preparation of the variant.Type: ApplicationFiled: June 28, 2010Publication date: November 11, 2010Inventors: MARIE ESKLING, Klaus Gregorius Nielsen
-
Patent number: 7820633Abstract: A method is disclosed for inducing cell-mediated immunity against cellular antigens. More specifically, the invention provides for a method for inducing cytotoxic T-lymphocyte immunity against weak antigens, notably self-proteins. The method entails that antigen presenting cells are induced to present at least one CTL epitope of the weak antigen and at the same time presenting at least one foreign T-helper lymphocyte epitope. In a preferred embodiment, the antigen is a cancer specific antigen, e.g. PSM, Her2, or FGF8b. The method can be exercised by using traditional polypeptide vaccination, but also by using live attenuated vaccines or nucleic acid vaccination. The invention furthermore provides immunogenic analogues of PSM, Her2 and FGF8b, as well as nucleic acid molecules encoding these analogues. Also vectors and transformed cells are disclosed. The invention also provides for a method for identification of immunogenic analogues of weak or non-immunogenic antigens.Type: GrantFiled: May 19, 2003Date of Patent: October 26, 2010Assignee: BN Immunotherapeutics, Inc.Inventors: Lucilla Steinaa, Jesper Haaning, Iben Dalum, Peter Birk, Dana Leach, Klaus Gregorius Nielsen, Gunilla Karlsson, Anand Gautam, Søren Mouritsen
-
Patent number: 7807441Abstract: A method is disclosed for inducing cell-mediated immunity against cellular antigens. More specifically, the invention provides for a method for inducing cytotoxic T-lymphocyte immunity against weak antigens, notably self-proteins. The method entails that antigen presenting cells are induced to present at least one CTL epitope of the weak antigen and at the same time presenting at least one foreign T-helper lymphocyte epitope. In a preferred embodiment, the antigen is a cancer specific antigen, e.g. PSM, Her2, or FGF8b. The method can be exercised by using traditional polypeptide vaccination, but also by using live attenuated vaccines or nucleic acid vaccination. The invention furthermore provides immunogenic analogues of PSM, Her2 and FGF8b, as well as nucleic acid molecules encoding these analogues. Also vectors and transformed cells are disclosed. The invention also provides for a method for identification of immunogenic analogues of weak or non-immunogenic antigens.Type: GrantFiled: August 11, 2005Date of Patent: October 5, 2010Assignee: BN Immunotherapeutics, Inc.Inventors: Lucilla Steinaa, Søren Mouritsen, Anand Gautam, Iben Dalum, Jesper Haaning, Dana Leach, Klaus Gregorius Nielsen, Gunilla Karlsson, Peter Birk Rasmussen