Abstract: Compounds which possess a complementary structure to a desired molecule, such as a biomolecule, in particular polymeric or oligomeric compounds, which are useful as in vivo or in vitro diagnostic and therapeutic agents are provided. Also, various methods for producing such compounds are provided. These polymeric or oligomeric compounds are useful in particular as antimicrobial agents, receptor, hormone or enzyme agonists and antagonists.

Abstract: Polymer particles are formed containing molecular memory recognition sites and magnetically susceptible components. The particles are prepared by co-polymerizing one or more monomers and a cross-linking agent in the presence of at least one imprint molecule and at least one magnetically susceptible component such as iron oxide or nickel oxide, and removing the imprint molecule to form molecular memory recognition sites. The particles are also prepared by co-polymerizing the monomer and cross-linking agent in the presence of the imprint molecule to produce particles, removing the imprint molecule and associating magnetically susceptible components with the particles. The particles may also be prepared containing selective adsorbents such as cells or antibodies. The particles are used for selective adsorption of a product such as separating and resolving two different enantiomeric forms due to one of the forms adsorbing to a memory recognition site created by using the form as an imprint molecule.

Abstract: This invention relates to molecularly imprinted polymers (MIPs) using an amide as the hydrogen bonding functional group. The amide MIPs made according to this invention exhibited enantiomeric recognition of the imprinted species when evaluated by high performance liquid chromatography (HPLC) even in an aqueous phase. In addition, the amide MIPs demonstrated improved enantiomeric resolution and load capacity.

Abstract: The invention relates to artificial antibodies that are prepared by molecular imprinting, where methacrylic acid, ethylene glycol dimethacrylate and a print molecule are combined to form an artificial antibody having spatially positioned binding sites dictated by the corticosteroid print molecule, and the antibodies can be used in separation and analytical procedures.

Abstract: Compounds which possess a complementary structure to a desired molecule, such as a biomolecule, in particular polymeric or oligomeric compounds, which are useful as in vivo or in vitro diagnostic and therapeutic agents are provided. Also, various methods for producing such compounds are provided. These polymeric or oligomeric compounds are useful in particular as antimicrobial agents, receptor, hormone or enzyme agonists and antagonists.

Abstract: Compounds which possess a complementary structure to a desired molecule, such as a biomolecule, in particular polymeric or oligomeric compounds, which are useful as in vivo or in vitro diagnostic and therapeutic agents are provided. Also, various methods for producing such compounds are provided. These polymeric or oligomeric compounds are useful in particular as antimicrobial agents, receptor, hormone or enzyme agonists and antagonists.

Abstract: The claimed invention is directed to a molecularly imprinted support formed from at least two distinct acylic monomers and at least one imprinted molecule. The support comprises beads having a uniform surface for reproducible presentation thereon.

Abstract: A suspension polymerization technique is provided for molecular imprinting based on the use of a perfluorocarbon liquid as the dispersing phase that does not interfere with the interactions between functional monomers and print molecules required for the recognition process during molecular imprinting, and provides controllable particle sizes within less than about 5 .mu.m to about 100 .mu.m.

Abstract: The claimed invention is directed to stabilizers, polymers, and emulsifiers useful in preparing molecularly imprinted materials having recognition capabilities corresponding to the imprint molecule.

Abstract: The present invention comprises a method Of detecting and quantifying components separated by capillary electrophoresis or other techniques using thin capillaries in the separation. The method of the present invention comprises irradiating the whole or large parts of the capillary at short time intervals and detecting the fluorescence originating from the sample components, preferably by a CCD detector (charge coupled device). Thereby, an instantaneous image of the progress of the separation process is obtained. The present invention further comprises an apparatus for performing this detection.

Abstract: A method for separating enantiomers of a derivative of an aryloxipropanolamine is disclosed, characterized in that the derivative is contacted with a chiral solid-phase chromatography material containing molecular imprints of an optically pure enantiomer of the derivative to be separated.

Abstract: A novel synthesis of N-acryloyl-tris-(hydroxymethyl)aminomethane (NAT) is described. The polymerization kinetics and transparency of the poly(NAT) gels crosslinked by a crosslinker (CL) e.g. N,N'-methylenebisacrylamide (Bis), i.e. poly(NAT+Bis) polymer, are shown. Poly(NAT+Bis) gradient (4-24%) gels were prepared and found to resolve native proteins according to their size. The exclusion limit of these gels is over 3.times.10.sup.6 Da. This is more than threefold higher than the exclusion limit of the polyacrylamide gradient gel of the same concentration. Therefore poly(NAT+CL) gels are better than polyacrylamide gels for the resolution of large proteins. It was demonstrated that poly(NAT+CL)-polyacrylamide composite gels could be prepared. The poly(NAT+CL) gels were found to be advantageous also for isoelectric focussing in carrier ampholytes or immobilized pH gradients. Poly(NAT+CL) gels are also very good for separation of nucleic acids.

Abstract: A method for detecting a glycoprotein using a solid support is disclosed where the glycoprotein is oxidized by periodate, polyacrylic polyhydrazide which is a copolymer having repeating units possessing a hydrazide group and repeating units possessing hydroxyl groups is coupled to the oxidized glycoprotein and a glycoenzyme or radioactive compound containing aldehyde groups or activated ketone groups is coupled to the polyacrylic polyhydrazide which allows for detection of the glycoprotein. The glycoprotein may be directly attached to the solid support or may be bound to an antigen which is immobilized on the solid support.

Abstract: A Triqonopsis variabilis D-amino acid oxidase in substantially pure form and active against cephalosporin C is disclosed. This D-amino acid oxidase is isolated from Trigonopsis variabilis by a method which is performed in three steps, namely:(a) acidifying and heating a crude cell extract of Trigonopsis variabilis to obtain a precipitate and supernatant fraction;(b) treating said supernatant fraction obtained in step (a) with sufficient ammonium sulfate to obtain a second precipitate, said second precipitate containing the D-amino acid oxidase of claim 1; and(c) resuspending the precipitate obtained in step (b) and collecting the D-amino acid oxidase by isoelectric precipitation.

Abstract: A method of preparing synthetic enzymes and synthetic antibodies in which method use is made of the so called molecular imprinting method, is described. As imprinting molecules use is made of transition state analogues, substrate-like compounds and antigen-like molecules, respectively. There is also described the use of the so prepared enzymes and antibodies.

Abstract: A chiral two-phase system for resolution of racemic mixtures, or for separation of diastereomers, is disclosed. The system comprises two immiscible liquid phases and one or more enantioselectively binding chiral components, each of which is substantially in one of said phases.Also disclosed is a method for chiral resolution of racemic mixtures, or for separation of diastereomers. Use is here made of the fact that different enantiomers are partitioned differently between the phases in the above-mentioned two-phase system in that one of the enantiomers is selectively bound to one of the chiral components which is substantially in one of said phases.

Abstract: The apparatus for detecting gases comprises a photoacoustic gas detector 35, in which the intensity-modulated absorbed test light beam 32' is converted into noise and detected by means of microphone 36. The photoacoustic gas detector 35 is acoustically decoupled from the gas collecting point 54 by a gas-permeable diaphragm 52 or a rigid, porous material 52'. The gas chamber 35' of photoacoustic detector 35 can be scavenged by means of supply lines 38, 38' and valves 56, 56'. It is advantageous to use a thermal source 32 intensity-modulated by phase lag. The signal is processed at microphone 36 by means of a N-path filter comprising cyclic switch 63 and R-elements 62, 65, 65'. The apparatus can be used for detecting CO.sub.2 and CO.

Abstract: Viable biomaterial such as animal cells, plant cells, bacteria, algae or fungi are immobilized with retained ability of growth by encapsulation in polymer beads. Encapsulation is carried out by adding the biomaterial to an aqueous solution of a polymer such as agar, agarose, carrageenan, chitosan, gelatin, collagen or fibrinogen, dispersing the solution in a water-insoluble dispersion medium such as soybean oil, tri-n-butylphosphate, liquid silicone, paraffin oil or phthalic acid dibutylester and allowing the polymer to gel.

Abstract: A separation agent composed of a water insoluble solid substance with a convatently bound lectin. Said solid substance is structurally composed of a water insoluble silica or silicate material as basic material and via a linkage to silicon atoms in the silica or silicate material, a covatently bound lectin.

Abstract: An intravascularly administrable, magnetically responsive nanosphere or nanoparticle made up of a crystalline carbohydrate matrix, preferably starch, enclosing a magnetic material, is described. The nanosphere or nanoparticle is produced by dissolving a carbohydrate together with a magnetic material to form a solution which is emulsified in a hydrophobic solvent from which the carbohydrate is crystallized.The resulting magnetic nanospheres having an average diameter not exceeding 1500 nm are capable of transporting pharmacologically active substances and can be injected intravenously for subsequent concentration in a part of the body by means of a magnetic field.