Patents by Inventor Knud Vad

Knud Vad has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • N-terminally extended proteins expressed in yeast
    Patent number: 6500645
    Abstract: The present invention relates to polypeptides expressed and processed in yeast, a DNA construct comprising a DNA sequence encoding such polypeptides, vectors carrying such DNA fragments and yeast cells transformed with the vectors, as well as a process of producing heterologous proteins in yeast.
    Type: Grant
    Filed: June 2, 1999
    Date of Patent: December 31, 2002
    Assignee: Novo Nordisk A/S
    Inventors: Thomas Børglum Kjeldsen, Per Balschmidt, Annette Frost Pettersson, Knud Vad, Jakob Brandt, Svend Havelund
  • Method of making proteins in transformed yeast cells
    Publication number: 20020192753
    Abstract: The present invention relates to a method for expressing heterologous proteins or polypeptides in yeast by culturing a transformed yeast strain which does not contain a functional antibiotic resistance marker gene.
    Type: Application
    Filed: February 14, 2002
    Publication date: December 19, 2002
    Inventors: Thomas Kjeldsen, Knud Vad
  • Method of making proteins in transformed yeast cells
    Patent number: 6358705
    Abstract: The present invention relates to a method for expressing heterologous proteins or polypeptides in yeast by culturing a transformed yeast strain which does not contain a functional antibiotic resistance marker gene.
    Type: Grant
    Filed: July 6, 1999
    Date of Patent: March 19, 2002
    Assignee: Novo Nordisk A/S
    Inventors: Thomas Kjeldsen, Knud Vad
  • Process for making desired polypeptides in yeast
    Patent number: 6183989
    Abstract: The invention describes a process for making desired polypeptides in yeast. The desired products are expressed as leader bound polypeptides connected by means of a monobasic processing site. The desired polypeptide is cleaved from the leader either in vivo or in vitro by enzymatic cleavage.
    Type: Grant
    Filed: March 3, 1999
    Date of Patent: February 6, 2001
    Assignee: Novo Nordisk A/S
    Inventors: Jacob Brandt, Knud Vad
  • Method for the production of polypeptides
    Patent number: 6110703
    Abstract: The present invention relates to a novel method for the production of short chain polypeptides, including polypeptides having up to 3 disulfide bonds and/or structures rich in basic amino acid residues, and open structured short chain polypeptides, e.g. glucagon, glucagon like peptides and their functional analogues, in genetically modified yeast cells, said genetically modified yeast cells, and a method for the preparation of said yeast cells.
    Type: Grant
    Filed: July 7, 1997
    Date of Patent: August 29, 2000
    Assignee: Novo Nordisk A/S
    Inventors: Michi Egel-Mitani, Jakob Brandt, Knud Vad
  • Synthetic leader peptide sequences
    Patent number: 5795746
    Abstract: The present invention relates to a DNA expression cassette comprising the following sequence:5'-P-SP-LS-PS-*gene*-(T).sub.i -3'whereinP is a promoter sequence,SP is a DNA sequence encoding a signal peptide,LS is a DNA sequence encoding a leader peptide of formula I:GlnProIle(Asp/Glu)(Asp/Glu)X.sup.1 (Glu/Asp)X.sup.2 AsnZ(Thr/Ser)X.sup.3 (SEQ ID NO:77) (I)whereinX.sup.l is a peptide bond or a codable amino acid;X.sup.2 is a peptide bond, a codable amino acid or a sequence of up to 4 codable amino acids which may be the same or different;Z is a codable amino acid except Pro; andX.sup.3 is a sequence of from 4 to 30 codable amino acids which may be the same or different;PS is a DNA sequence encoding a processing site;*gene* is a DNA sequence encoding a polypeptide;T is a terminator sequence; andi is 0 or 1.
    Type: Grant
    Filed: January 8, 1997
    Date of Patent: August 18, 1998
    Assignee: Novo Nordisk A/S
    Inventors: Thomas B.o slashed.rglum Kjeldsen, Knud Vad
  • Synthetic leader peptide sequences
    Patent number: 5639642
    Abstract: The present invention relates to a DNA expression cassette comprising the following sequence:5'-P-SP-LS-PS-*gene*-(T).sub.i -3'whereinP is a promoter sequence,SP is a DNA sequence encoding a signal peptide,LS is a DNA sequence encoding a leader peptide of formula I:GinProIle(Asp/Glu)(Asp/Glu)X.sup.1 (Glu/Asp)X.sup.2 AsnZ(Thr/Ser)X.sup.3 (SEQ ID NO: 77) (I)whereinX.sup.1 is a peptide bond or a codable amino acid;X.sup.2 is a peptide bond, a codable amino acid or a sequence of up to 4 codable amino acids which may be the same or different;Z is a codable amino acid except Pro; andX.sup.3 is a sequence of from 4 to 30 codable amino acids which may be the same or different;PS is a DNA sequence encoding a processing site;*gene* is a DNA sequence encoding a polypeptide;T is a terminator sequence; andi is 0 or 1.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: June 17, 1997
    Assignee: Novo Nordisk A/S
    Inventors: Thomas B.o slashed.rglum Kjeldsen, Knud Vad