Abstract: An isolated expression enhancer active in a plant, portion of a plant or plant cell, the expression enhancer is provided. The isolated expression enhancer may be selected from the group consisting of nbEPI42 (SEQ ID NO:1); nbSNS46 (SEQ ID NO:2); nbCSY65 (SEQ ID NO:3); nbHEL40 (SEQ ID NO:4); and nbSEP44 (SEQ ID NO:5). Methods for using the isolated expression enhancer are also provided.

Abstract: An isolated expression enhancer active in a plant, portion of a plant or plant cell, the expression enhancer is provided. The isolated expression enhancer may be selected from the group consisting of nbMT78 (SEQ ID NO:1); nbATL75 (SEQ ID NO:2); nbDJ46 (SEQ ID NO:3); nbCHP79 (SEQ ID NO:4); nbEN42 (SEQ ID NO:5); atHSP69 (SEQ ID NO:6); atGRP62 (SEQ ID NO:7); atPK65 (SEQ ID NO:8); atRP46 (SEQ ID NO:9); nb30S72 (SEQ ID NO:10); nbGT61 (SEQ ID NO:11); nbPV55 (SEQ ID NO:12); nbPPI43 (SEQ ID NO:13); nbPM64 (SEQ ID NO:14); and nbH2A86 (SEQ ID NO:15). Methods for using the isolated expression enhancer are also provided.

Abstract: The purpose of the present invention is to provide technology for identifying a 5? UTR having excellent translation ability while taking into account 5? UTR variants in monocotyledons, providing a DNA molecule that codes for the 5? UTR, and efficiently producing a recombinant protein in the monocotyledons. It is possible to efficiently produce a recombinant protein in a monocotyledon by using a nucleic acid structure in a polynucleotide that codes for a foreign protein, said structure comprising a base sequence among the base sequences shown in sequences 1-4 and linking to a 5? UTR or a variant thereof.

Abstract: The present invention addresses the problem of developing a DNA molecule encoding a 5?UTR that enables the high-level expression of a recombinant protein in a plant, and providing a technique for producing a recombinant protein efficiently. A nucleic acid construct composed of a polynucleotide encoding a protein and a 5?UTR comprising a nucleotide sequence represented by SEQ ID NO: 1 or 2 or a variant of the 5?UTR, wherein the 5?UTR is ligated to the polynucleotide. By using the nucleic acid construct, it becomes possible to efficiently produce a recombinant protein in a plant.

Abstract: An object of the present invention is to identify sequence features of the 5? UTR, which are involved in changes in the translational state of plants under environmental stresses, and to provide a recombinant DNA molecule, an expression vector, a transformant, and the like, having the above sequence features.

Abstract: An object of this invention is to produce a protein by efficiently culturing plant cells while escaping mRNA translational repression in cultured plant cells under stress caused by lack of conditions essentially required for the growth (e.g., nutrient-starvation stress and hypoxic stress).

Abstract: An object of the present invention is to identify sequence features of the 5? UTR, which are involved in changes in the translational state of plants under environmental stresses, and to provide a recombinant DNA molecule, an expression vector, a transformant, and the like, having the above sequence features.

Abstract: A cleaning apparatus includes a plurality of air nozzles that discharge air from above each of the distal ends of a suction nozzle and a discharge nozzle, the suction nozzle sucking in at least some of reaction liquid undergoing a heterogeneous reaction, the discharge nozzle discharging a predetermined B/F cleaning fluid, the air nozzles being provided corresponding respectively to a plurality of pairs of the suction and the discharge nozzles. The apparatus includes an air supply unit that supplies air to the plurality of air nozzles at a time; a plurality of pressure sensors each of which detects a pressure of air which is supplied by the air supply unit and which is in the vicinity of each proximal end of the air nozzles; and a control unit that individually controls the B/F-cleaning-fluid discharging operation of the discharge nozzles based on the respective detected pressures and changes in the pressures.

Abstract: The present invention is to provide a gene having asymmetric hydrolase activity which is useful for synthesis of an optically active carboxylic acid, its antipode ester, and lactone, and a hydroxycarboxylic ester asymmetric hydrolase enzyme (EnHCH) derived from Enterobacter sp. DS-S-75 strain (FERM BP-5494) which is bacteria belonging to the genus Enterobacter, a EnHCH gene shown by base sequence of SEQ. ID. NO: 1, a gene encoding a protein having an amino acid sequence of SEQ. ID. NO: 2, and E. coli DH5? (pKK-EnHCH) deposited to International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology as a deposition No. FERM BP-08466.

Abstract: The present invention is to provide a gene having asymmetric hydrolase activity which is useful for synthesis of an optically active carboxylic acid, its antipode ester, and lactone, and a hydroxycarboxylic ester asymmetric hydrolase enzyme (EnHCH) derived from Enterobacter sp. DS-S-75 strain (FERM BP-5494) which is bacteria belonging to the genus Enterobacter, a EnHCH gene shown by base sequence of SEQ. ID. NO: 1, a gene encoding a protein having an amino acid sequence of SEQ. ID. NO: 2, and E. coli DH5? (pKK-EnHCH) deposited to International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology as a deposition No. FERM BP-08466.

Abstract: The present invention is to provide a gene having asymmetric hydrolase activity which is useful for synthesis of an optically active carboxylic acid, its antipode ester, and lactone, and a hydroxycarboxylic ester asymmetric hydrolase enzyme (EnHCH) derived from Enterobacter sp. DS-S-75 strain (FERM BP-5494) which is bacteria belonging to the genus Enterobacter, a EnHCH gene shown by base sequence of SEQ.ID.NO: 1, a gene encoding a protein having an amino acid sequence of SEQ.ID.NO: 2, and E. coli DH5? (pKK-EnHCH) deposited to International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology as a deposition No. FERM BP-08466.

Abstract: The present invention is to provide a gene having asymmetric hydrolase activity which is useful for synthesis of an optically active carboxylic acid, its antipode ester, and lactone, and a hydroxycarboxylic ester asymmetric hydrolase enzyme (EnHCH) derived from Enterobacter sp. DS-S-75 strain (FERM BP-5494) which is bacteria belonging to the genus Enterobacter, a EnHCH gene shown by base sequence of SEQ.ID.NO: 1, a gene encoding a protein having an amino acid sequence of SEQ.ID.NO: 2, and E. coli DH5&agr; (pKK-EnHCH) deposited to International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology as a deposition No. FERM BP-08466.

Abstract: A method useful for stable expression of an exogenous gene, introduced into a plant, is provided. By introducing an exogenous gene and 5′ upstream sequence of tobacco dehydogenase concurrently, stable expression of the exogenous gene introduced into a plant can be achieved.

Abstract: A method for the stable expression of an introduced exogenous gene in a plant or plant cell is provided. Stable expression of an exogenous gene that was introduced was achieved by operably linking an upstream sequence of sea urchin arylsulfatase gene as an insulator.