Patents by Inventor Kohichiro Tsuji

Kohichiro Tsuji has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for production of eosinophil from pluripotent stem cell
    Patent number: 9404082
    Abstract: The present invention relates to a method for producing human eosinophils from human pluripotent stem cells. More specifically, the present invention provides a method for producing human eosinophils from human pluripotent stem cells, which method comprises the steps of: (1) co-culturing, in the presence of VEGF, human pluripotent stem cells with cells separated from the AGM region of a mammalian fetus; (2) performing suspension culture using a medium comprising IL-3, IL-6, Flt3 ligand, SCF, TPO and serum; (3) performing suspension culture using a medium comprising IL-3, SCF, GM-CSF and serum; and, optionally, (4) performing suspension culture using a medium comprising IL-3, IL-5 and serum.
    Type: Grant
    Filed: December 2, 2011
    Date of Patent: August 2, 2016
    Assignee: KYOTO UNIVERSITY
    Inventors: Tatsutoshi Nakahata, Kohichiro Tsuji, Feng Ma, Hirohisa Saito, Kenji Matsumoto
  • METHOD FOR PRODUCTION OF EOSINOPHIL FROM PLURIPOTENT STEM CELL
    Publication number: 20150104820
    Abstract: The present invention relates to a method for producing human eosinophils from human pluripotent stem cells. More specifically, the present invention provides a method for producing human eosinophils from human pluripotent stem cells, which method comprises the steps of: (1) co-culturing, in the presence of VEGF, human pluripotent stem cells with cells separated from the AGM region of a mammalian fetus; (2) performing suspension culture using a medium comprising IL-3, IL-6, Flt3 ligand, SCF, TPO and serum; (3) performing suspension culture using a medium comprising IL-3, SCF, GM-CSF and serum; and, optionally, (4) performing suspension culture using a medium comprising IL-3, IL-5 and serum.
    Type: Application
    Filed: December 2, 2011
    Publication date: April 16, 2015
    Applicant: KYOTO UNIVERSITY
    Inventors: Tatsutoshi Nakahata, Kohichiro Tsuji, Feng Ma, Hirohisa Saito, Kenji Matsumoto
  • Method for producing mast cells from pluripotent stem cells
    Patent number: 8741644
    Abstract: The present invention relates to a method for producing human mast cells from human pluripotent stem cells. More particularly, the present invention provides a method for producing human mast cells from human pluripotent stem cells, comprising the steps of: (a) culturing human pluripotent stem cells under a condition suitable for promoting differentiation of the human pluripotent stem cells into hematopoietic progenitor cells expressing CD34; and (b) culturing the cells obtained in step (a) in the presence of hematopoietic factors comprising thrombopoietin (TPO) and Flt3 ligand.
    Type: Grant
    Filed: September 8, 2010
    Date of Patent: June 3, 2014
    Assignee: Kyoto University
    Inventors: Tatsutoshi Nakahata, Kohichiro Tsuji, Feng Ma
  • METHOD FOR PRODUCING MAST CELLS FROM PLURIPOTENT STEM CELLS
    Publication number: 20120208206
    Abstract: The present invention relates to a method for producing human mast cells from human pluripotent stem cells. More particularly, the present invention provides a method for producing human mast cells from human pluripotent stem cells, comprising the steps of: (a) culturing human pluripotent stem cells under a condition suitable for promoting differentiation of the human pluripotent stem cells into hematopoietic progenitor cells expressing CD34; and (b) culturing the cells obtained in step (a) in the presence of hematopoietic factors comprising thrombopoietin (TPO) and Flt3 ligand.
    Type: Application
    Filed: September 8, 2010
    Publication date: August 16, 2012
    Inventors: Tatsutoshi Nakahata, Kohichiro Tsuji, Feng Ma