Abstract: A cell line of Accession No. NITE P-03458. A producing method of a cell line includes: a transfection step of transfecting a vector into a cell, where the vector includes a nucleic acid sequence encoding a nonstructural protein of a coronavirus, a reporter protein including a drug-resistant protein, and a structural protein of the coronavirus; and a selection step of performing selection on the cell obtained in the transfection step using a drug.

Abstract: The present invention provides an anti-hepatitis C virus composition that includes a substance that suppresses the expression or function of a PA28? gene, a method for preventing hepatitis C viral infection or suppressing hepatitis C virus growth that includes the step of administering the composition to a subject, and a method for screening an effective component of an anti-hepatitis C virus composition that includes the step of selecting a substance that inhibits the expression or function of a PA28? gene.

Abstract: An easy and efficient screening method for an agent useful for prevention and/or treatment of hepatitis C virus-related disease, and a prophylactic and/or therapeutic agent for hepatitis C virus-related disease obtained by the method. The screening method comprises a step of examining the inhibitory activity of an test substance on the protein-protein interaction between hepatitis C virus core protein and PA28.gamma,. The method can be carried out by using transcriptional activity of a lipid metabolism-control factor as indicator. The test substance used in the method may have an activity to inhibit expression or function of the PA28.gamma. gene. The hepatitis C virus-related diseases include fatty liver, acute hepatitis, chronic hepatitis, liver cirrhosis, liver cancer, and insulin-resistant diseases. The method is extremely useful as a method of obtaining an agent superior in preventive and therapeutic activity particularly to diseases such as fatty liver and liver cancer.

Abstract: The present invention provides an anti-hepatitis C virus composition that includes a substance that suppresses the expression or function of a PA28? gene, a method for preventing hepatitis C viral infection or suppressing hepatitis C virus growth that includes the step of administering the composition to a subject, and a method for screening an effective component of an anti-hepatitis C virus composition that includes the step of selecting a substance that inhibits the expression or function of a PA28? gene.

Abstract: Provided is a screening method being able to screen an agent useful for prevention and/or treatment of hepatitis C virus-related disease easily and efficiently and a prophylactic and/or therapeutic agent for hepatitis C virus-related disease obtained by the method. The screening method for a prophylactic and/or therapeutic agent for hepatitis C virus-related disease according to the present invention is characterized by comprising a a step of examining the inhibitory activity of an test substance on the protein-protein interaction between hepatitis C virus core protein and PA28?. The method can be carried out, for example, by using transcriptional activity of a lipid metabolism-control factor as indicator. The test substance used in the method may have an activity to inhibit expression or function of the PA28? gene. The hepatitis C virus-related diseases include hepatitis C, fatty liver, acute hepatitis, chronic hepatitis, liver cirrhosis, liver cancer, insulin-resistant diseases, and the like.