Patents by Inventor Kohji Nishida

Kohji Nishida has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12365950
    Abstract: A method for detecting conjunctival diseases such as conjunctival MALT lymphoma, and an aging biomarker that serves as an indicator of the aging state of a subject are provided. The method for detecting conjunctival diseases comprises a step of comparing a microbial community structure of a microbiota in an ocular surface tissue specimen sampled from a healthy person with a microbial community structure of a microbiota in an ocular surface tissue specimen sampled from a subject to determine that the ocular surface tissue specimen of the subject has an indication of the conjunctival diseases. The aging biomarker a comprises bacterial species which belongs to the Corynebacteriaceae family or the Propionibacteriales family in an ocular surface tissue.
    Type: Grant
    Filed: June 28, 2019
    Date of Patent: July 22, 2025
    Assignees: Osaka University, Otsuka Pharmaceutical Co., Ltd.
    Inventors: Kohji Nishida, Noriyasu Hashida, Tetsuya Iida, Shota Nakamura, Daisuke Motooka, Kazunobu Asao, Satoru Ando
  • Publication number: 20250179428
    Abstract: The present invention relates to a method for inducing the differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lineage in a serum-free medium without using feeder cells and inducing the differentiation of the resultant ocular surface ectodermal lineage cells into corneal epithelial cells.
    Type: Application
    Filed: February 5, 2025
    Publication date: June 5, 2025
    Applicant: Osaka University
    Inventors: Kohji NISHIDA, Ryuhei HAYASHI, Yuki ISHIKAWA
  • Patent number: 12291727
    Abstract: Provided is a method for producing a stem cell-derived lacrimal gland tissue, the method comprising isolating SSEA4 and CD104 double positive cells from a self-formed ectodermal autonomous multi-zone (SEAM) cell population derived from pluripotent stem cells and three-dimensionally culturing the isolated cells in a medium with epidermal growth factor (EGF) and a ROCK inhibitor to produce a cell population expressing a lacrimal gland-related protein. The present invention provides a lacrimal gland organoid produced from pluripotent stem cells including iPS cells and thus is very useful for cell-based regenerative therapy for lacrimal gland-related diseases and cell-based research on the diseases.
    Type: Grant
    Filed: March 28, 2019
    Date of Patent: May 6, 2025
    Assignee: OSAKA UNIVERSITY
    Inventors: Kohji Nishida, Ryuhei Hayashi, Toru Okubo, Yoichi Honma
  • Patent number: 12272459
    Abstract: A medical diagnostic apparatus includes a receiver circuit that receives three-dimensional data of a subject's eye, and a processor configured to separate portions of the three-dimensional data into separate segments, perform processing differently on each of the separate segments, and combine the separately processed segments to produce a segmented three-dimensional data set. The processor is further configured to generate at least one diagnostic metric from the segmented three-dimensional data set, and the processor is further configured to evaluate a pathological condition based on the at least one diagnostic metric. Related methods and computer readable media are also disclosed.
    Type: Grant
    Filed: October 5, 2021
    Date of Patent: April 8, 2025
    Assignees: Topcon Corporation, Osaka University
    Inventors: Kohji Nishida, Atsuya Miki, Kazuichi Maruyama, Ryo Kawasaki, Song Mei, Zaixing Mao, Zhenguo Wang, Kinpui Chan, Xin Sui
  • Patent number: 12247219
    Abstract: The present invention relates to a method for inducing the differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lineage in a serum-free medium without using feeder cells and inducing the differentiation of the resultant ocular surface ectodermal lineage cells into corneal epithelial cells.
    Type: Grant
    Filed: June 15, 2021
    Date of Patent: March 11, 2025
    Assignee: Osaka University
    Inventors: Kohji Nishida, Ryuhei Hayashi, Yuki Ishikawa
  • Patent number: 12202218
    Abstract: The present invention provides a new method for producing a lens to be fitted to an eye in a simple manner at a low cost. In order to achieve the aforementioned object, the present invention provides a method for producing a lens to be fitted to an eye, including the step of: hydrophilizing a surface of a polymer lens by reacting with a compound radical, wherein the compound radical is a radical containing one element selected from the group consisting of Group 15 elements and Group 16 elements, and a Group 17 element.
    Type: Grant
    Filed: June 20, 2019
    Date of Patent: January 21, 2025
    Assignee: Osaka University
    Inventors: Kohji Nishida, Koichi Baba, Shizuka Koh, Kei Ohkubo, Haruyasu Asahara, Tsuyoshi Inoue, Hiroshi Uyama, Taka-Aki Asoh
  • Publication number: 20240274295
    Abstract: There are provided a highly versatile method for screening for retinopathy of prematurity, a screening apparatus, and a trained model that are capable of accurately predict the progression of retinopathy of prematurity at appropriate timing. There is provided a method for screening for retinopathy of prematurity to predict the progression of retinopathy of prematurity, the method including a treatment determination step of determining whether or not treatment is indicated for retinopathy of prematurity after a predetermined number of days after birth based on premature infant information including postnatal time-series data on weight, height, and vital signs of a premature infant whose gestational age is less than a predetermined week.
    Type: Application
    Filed: May 31, 2022
    Publication date: August 15, 2024
    Inventors: Yoko FUKUSHIMA, Kohji NISHIDA, Ryosuke KOJIMA, Yasushi OKUNO
  • Patent number: 12035973
    Abstract: A method for detecting a stressed state, including a step of detecting a stressed state of a subject based on a tomographic image of a choroid of the subject, and a stress detection apparatus including: an image acquisition unit that acquires a tomographic image of a choroid of a subject; a calculation unit that calculates a choroidal thickness or a volume of the choroid based on the tomographic image; and a detection unit that detects a stressed state of the subject based on the choroidal thickness or the volume of the choroid.
    Type: Grant
    Filed: January 25, 2019
    Date of Patent: July 16, 2024
    Assignee: OSAKA UNIVERSITY
    Inventors: Kohji Nishida, Kazuichi Maruyama, Noriyasu Hashida, Reiko Kobayashi
  • Patent number: 12013365
    Abstract: Provided are a virus measuring method, a virus measuring device, a virus determining program, a stress determining method, and a stress determining device. A virus measuring method includes a contact step of bringing a liquid specimen containing a body fluid of a subject and an electrolytic solution into contact with each other via a through-hole portion formed in a separating wall, a current measuring step of applying a voltage to the liquid specimen and the electrolytic solution with respect to the through-hole portion and obtaining a waveform of an ionic current flowing through the through-hole portion, and a virus determining step of determining the kind of a virus contained in the body fluid on the basis of the waveform. In the virus determining step, the kind of the virus is determined by comparing the waveform with waveform information that corresponds to a known virus and is obtained beforehand.
    Type: Grant
    Filed: July 18, 2019
    Date of Patent: June 18, 2024
    Assignee: OSAKA UNIVERSITY
    Inventors: Kohji Nishida, Noriyasu Hashida, Masateru Taniguchi, Makusu Tsutsui
  • Patent number: 11839697
    Abstract: Provided is a means for identifying cells suitable for transplantation into a cornea, or a means for producing a cell population suitable for transplantation into a cornea using the same. A method for producing a cell population suitable for transplantation into a cornea, the method comprising the steps of (1) preparing a cell population cultured under conditions suitable for inducing differentiation into corneal endothelial cells, and (2) measuring the expression of at least one gene selected from the group consisting of POU6F2, LMX1B, and TFAP2B in the cell population.
    Type: Grant
    Filed: June 19, 2018
    Date of Patent: December 12, 2023
    Assignee: OSAKA UNIVERSITY
    Inventors: Kohji Nishida, Motokazu Tsujikawa, Susumu Hara, Satoshi Kawasaki, Masahito Yoshihara, Masayoshi Itoh, Hideya Kawaji
  • Publication number: 20230306568
    Abstract: A medical diagnostic apparatus includes a receiver circuit that receives three-dimensional volumetric data of a subject’s eye, and a processor configured to separate portions of the three-dimensional volumetric data into separate segments, perform processing differently on each of the separate segments, and combine the separately processed segments to produce an enhanced three-dimensional volumetric data set. The processor is further configured to generate at least one diagnostic metric from the enhanced three-dimensional volumetric data set, and the processor is further configured to evaluate a pathological condition based on the at least one diagnostic metric. Related methods and computer readable media are also disclosed.
    Type: Application
    Filed: June 22, 2021
    Publication date: September 28, 2023
    Applicants: Osaka University, Topcon Corporation
    Inventors: Kohji NISHIDA, Kazuichi MARUYAMA, Atsuya MIKI, Ryo KAWASAKI, Hirokazu SAKAGUCHI, Chikako HARA, Song MEI, Yasufumi FUKUMA, Zaixing MAO, Zhenguo WANG, Kinpui CHAN
  • Patent number: 11649433
    Abstract: The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.
    Type: Grant
    Filed: January 31, 2018
    Date of Patent: May 16, 2023
    Assignee: Osaka University
    Inventors: Kohji Nishida, Kiyotoshi Sekiguchi, Ryuhei Hayashi, Shun Shibata
  • Publication number: 20230105247
    Abstract: A medical diagnostic apparatus includes a receiver circuit that receives three-dimensional data of a subject's eye, and a processor configured to separate portions of the three-dimensional data into separate segments, perform processing differently on each of the separate segments, and combine the separately processed segments to produce a segmented three-dimensional data set. The processor is further configured to generate at least one diagnostic metric from the segmented three-dimensional data set, and the processor is further configured to evaluate a pathological condition based on the at least one diagnostic metric. Related methods and computer readable media are also disclosed.
    Type: Application
    Filed: October 5, 2021
    Publication date: April 6, 2023
    Applicants: Topcon Corporation, Osaka University
    Inventors: Kohji NISHIDA, Atsuya MIKI, Kazuichi MARUYAMA, Ryo KAWASAKI, Song MEI, Zaixing MAO, Zhenguo WANG, Kinpui CHAN, Xin SUI
  • Patent number: 11357798
    Abstract: The present invention relates to mesenchymal stem cell-derived microparticles having activity that promotes the growth of corneal epithelial stem cells and/or corneal epithelial cells, activity that maintains corneal epithelial stem cells in an undifferentiated state or promotes the formation of colonies thereby, and function that protects corneal epithelium.
    Type: Grant
    Filed: August 3, 2016
    Date of Patent: June 14, 2022
    Assignees: OSAKA UNIVERSITY, ROHTO PHARMACEUTICAL CO., LTD.
    Inventors: Kohji Nishida, Ryuuhei Hayashi, Yoichi Honma, Toru Okubo, Shun Shibata
  • Publication number: 20210371818
    Abstract: The present invention relates to a method for inducing the differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lineage in a serum-free medium without using feeder cells and inducing the differentiation of the resultant ocular surface ectodermal lineage cells into corneal epithelial cells.
    Type: Application
    Filed: June 15, 2021
    Publication date: December 2, 2021
    Applicant: Osaka University
    Inventors: Kohji NISHIDA, Ryuhei HAYASHI, Yuki ISHIKAWA
  • Publication number: 20210332324
    Abstract: The present invention provides a method for producing a corneal epithelial cell population for the fabrication of corneal epithelial cell sheets for transplantation, the method comprising the steps of: (1) preparing a cell population containing corneal epithelial cells; (2) subjecting the cell population prepared in step (1) to magnetic-activated cell sorting and collecting CD200-negative/SSEA-4-positive cells; and (3) bringing the cells collected in step (2) into contact with a laminin selected from the group consisting of a laminin having an ?3 chain, a laminin having an ?4 chain and a laminin having an ?5 chain, or an E8 fragment thereof, and collecting cells adherent thereto.
    Type: Application
    Filed: December 27, 2017
    Publication date: October 28, 2021
    Inventors: Kohji Nishida, Ryuhei Hayashi, Shun Shibata
  • Publication number: 20210290432
    Abstract: Provided is an inexpensive keratoconjunctival cover sheet that is comfortable and has good oxygen-permeability, contamination-resistance, wettability, handleability, and optical properties, and that can be used for medical treatment, visual correction, a cosmetic application, and the like. The keratoconjunctival cover sheet is constituted by a gel layer that includes metal ions and a water-soluble gelling agent exhibiting a gelation ability in the presence of the metal ions, and has a surface area capable of covering a cornea or keratoconjunctiva.
    Type: Application
    Filed: November 7, 2017
    Publication date: September 23, 2021
    Inventors: Koichi BABA, Kohji NISHIDA
  • Publication number: 20210285911
    Abstract: Provided are a virus measuring method, a virus measuring device, a virus determining program, a stress determining method, and a stress determining device. A virus measuring method includes a contact step of bringing a liquid specimen containing a body fluid of a subject and an electrolytic solution into contact with each other via a through-hole portion formed in a separating wall, a current measuring step of applying a voltage to the liquid specimen and the electrolytic solution with respect to the through-hole portion and obtaining a waveform of an ionic current flowing through the through-hole portion, and a virus determining step of determining the kind of a virus contained in the body fluid on the basis of the waveform. In the virus determining step, the kind of the virus is determined by comparing the waveform with waveform information that corresponds to a known virus and is obtained beforehand.
    Type: Application
    Filed: July 18, 2019
    Publication date: September 16, 2021
    Applicant: OSAKA UNIVERSITY
    Inventors: Kohji NISHIDA, Noriyasu HASHIDA, Masateru TANIGUCHI, Makusu TSUTSUI
  • Publication number: 20210263339
    Abstract: The present invention provides a new method for producing a lens to be fitted to an eye in a simple manner at a low cost. In order to achieve the aforementioned object, the present invention provides a method for producing a lens to be fitted to an eye, including the step of: hydrophilizing a surface of a polymer lens by reacting with a compound radical, wherein the compound radical is a radical containing one element selected from the group consisting of Group 15 elements and Group 16 elements, and a Group 17 element.
    Type: Application
    Filed: June 20, 2019
    Publication date: August 26, 2021
    Inventors: Kohji NISHIDA, Koichi BABA, Shizuka KOH, Kei OHKUBO, Haruyasu ASAHARA, Tsuyoshi INOUE, Hiroshi UYAMA, Taka-Aki ASOH
  • Patent number: 11066641
    Abstract: The present invention relates to a method for inducing the differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lineage in a serum-free medium without using feeder cells and inducing the differentiation of the resultant ocular surface ectodermal lineage cells into corneal epithelial cells.
    Type: Grant
    Filed: January 13, 2016
    Date of Patent: July 20, 2021
    Assignee: Osaka University
    Inventors: Kohji Nishida, Ryuhei Hayashi, Yuki Ishikawa