Abstract: The present invention provides a method for detecting multiple target nucleic acids, the method making it possible to amplify a plurality of genes using one reaction vessel containing therein one type of reaction solution and further using a single label. Computation is performed in accordance with the following formulas for every specific cycle and/or every cycle during amplification reaction using a kit for detecting the multiple target nucleic acids. (Formula 1): f1[n]=fhyb.1[n]/fden.1[n], (Formula 1?): f2[n]=fhyb.2[n]/fden.2[n], (Formula 2): Fr[n]=(a?f2 [n])/(a?f1 [n]).fhyb.1 [n]: Fluorescence intensity value in elongation step of first target nucleic acid detecting step. The same hereinafter.