Abstract: The present invention relates to: [1] a nonaqueous electrolytic solution containing a compound (A) represented by formula (I); and [2] a nonaqueous electrolytic solution battery including a positive electrode, a negative electrode, and the nonaqueous electrolytic solution.

Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.

Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.

Abstract: A cap of a linear guide device includes a receiving part configured to receive hitting of a hitting jig, a fitting part extending from the receiving part, having an outer diameter to be an interference with the counterbore part, and being press-fitted into the counterbore part, a foreign material receiving groove arranged at an outer circumference of the fitting part, and configured to receive a foreign material generated from the fitting part when the cap is hit into the counterbore part, and a gas discharge groove extending to reach an inside of the counterbore part from the foreign material receiving groove, with the fitting part being press-fitted into the counterbore part at the outer circumference of the fitting part, and configured to discharge a gas in the inside of the counterbore part to a foreign material receiving groove side.

Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.

Abstract: A cap of a linear guide device includes a receiving part configured to receive hitting of a hitting jig, a fitting part extending from the receiving part, having an outer diameter to be an interference with the counterbore part, and being press-fitted into the counterbore part, a foreign material receiving groove arranged at an outer circumference of the fitting part, and configured to receive a foreign material generated from the fitting part when the cap is hit into the counterbore part, and a gas discharge groove extending to reach an inside of the counterbore part from the foreign material receiving groove, with the fitting part being press-fitted into the counterbore part at the outer circumference of the fitting part, and configured to discharge a gas in the inside of the counterbore part to a foreign material receiving groove side.

Abstract: The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.

Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.

Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.

Abstract: A side seal for a linear guide apparatus including a guide rail, a slider, and a plurality of rolling elements includes seal members formed of different materials, a holder plate and covers. Both seal members have recesses formed in a first face of a base and fitted with a protrusion of the holder plate. Both seal members have respective lips directed in opposite directions separated from each other. A tip of the lip of the first seal member is formed to have a shape in plane contact with the face of the guide rail. A tip of the lip of the second seal member is formed to have a shape in line contact with the face of the guide rail.

Abstract: A side seal for a linear guide apparatus including a guide rail, a slider, and a plurality of rolling elements includes seal members formed of different materials, a holder plate and covers. Both seal members have recesses formed in a first face of a base and fitted with a protrusion of the holder plate. Both seal members have respective lips directed in opposite directions separated from each other. A tip of the lip of the first seal member is formed to have a shape in plane contact with the face of the guide rail. A tip of the lip of the second seal member is formed to have a shape in line contact with the face of the guide rail.

Abstract: The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.

Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.

Abstract: Genetically modified mammalian cells comprising a Tol2 transposon transferred into a chromosome can be obtained by co-transfecting mammalian cells with a Tol2 transposase encoded by a Tol2 transposon found in medaka fish, and a Tol2 transposon lacking this transposase.

Abstract: A transposase encoded by the Tol2 element; a polynucleotide encoding the same; a method of modifying the gene structure of a cell (preferably a vertebrate cell) by using the above protein; a method of modifying the function of a cell by modifying the gene structure thereof; and a cell having been modified in function by these methods. Also the structure of a cis element necessary in gene transfer is clarified and presented.

Abstract: A transposase encoded by the Tol2 element; a polynucleotide encoding the same; a method of modifying the gene structure of a cell (preferably a vertebrate cell) by using the above protein; a method of modifying the function of a cell by modifying the gene structure thereof; and a cell having been modified in function by these methods. Also the structure of a cis element necessary in gene transfer is clarified and presented.

Abstract: The present invention aims to smoothly and adequately adjust recording laser power while an increase in processing load during recording is suppressed. When a recording operation start request is received, OPC is executed with reference to a target ? value (?t) of a disc to set recording laser power Pws. Recording operation is performed based on the set recording laser power Pws. When a system is brought into an intermittent standby state, R-OPC is executed, so the recording laser power Pws is adjusted and set again. Then, its subsequent recording operation is performed based on the recording laser power Pws which is set again. When a duration of the recording operation exceeds a maximal waiting time Ts, the recording operation is suspended so that the system is set to a recording standby state. Then, the R-OPC is executed, so that the recording laser power Pws is adjusted and set again.

Abstract: A transposase encoded by the Tol2 element; a polynucleotide encoding the same; a method of modifying the gene structure of a cell (preferably a vertebrate cell) by using the above protein; a method of modifying the function of a cell by modifying the gene structure thereof; and a cell having been modified in function by these methods. Also the structure of a cis element necessary in gene transfer is clarified and presented.

Abstract: A semiconductor memory device includes: an ID code memory circuit configured to store ID code data bits in a non-volatile manner; an ID code generating circuit configured to generate an ID code based on the data bits stored in the ID code memory circuit and output it to an external terminal; a power supply voltage detecting circuit configured to detect a power supply voltage supplied from the external and supply a select signal to at least one of the ID code memory circuit and ID code generating circuit for selectively generating one of ID codes, data bits of which are different from each other at least in part in correspondence with power supply voltage levels.

Abstract: A transposase encoded by the Tol2 element; a polynucleotide encoding the same; a method of modifying the gene structure of a cell (preferably a vertebrate cell) by using the above protein; a method of modifying the function of a cell by modifying the gene structure thereof; and a cell having been modified in function by these methods. Also the structure of a cis element necessary in gene transfer is clarified and presented.