Abstract: A method of introducing solution into the wells of a multiwell plate comprised of a substrate having multiple wells on at least one principal surface thereof. The wells are imparted dimensions, shapes, and surface configurations such that when said multiwell plate is positioned in stationary fashion with the openings of said wells facing upward, said solution does not enter said wells even when the openings of said wells are covered by said solution, and the solution is positioned on said principal surface of said multiwell plate having wells and a centrifugal force oriented from the well opening toward the bottom is applied to introduce said solution into said wells. Provided is a method for conveniently filling vessels (wells) with a solution such as a reaction solution, even when the vessels (wells) number in excess of 1,000, and even when the dimension, shape, and surface configuration of the vessels (wells) do not permit fluid to flow into the vessels (wells).

Abstract: A method of introducing solution into the wells of a multiwell plate comprised of a substrate having multiple wells on at least one principal surface thereof. The wells are imparted dimensions, shapes, and surface configurations such that when said multiwell plate is positioned in stationary fashion with the openings of said wells facing upward, said solution does not enter said wells even when the openings of said wells are covered by said solution, and the solution is positioned on said principal surface of said multiwell plate having wells and a centrifugal force oriented from the well opening toward the bottom is applied to introduce said solution into said wells. Provided is a method for conveniently filling vessels (wells) with a solution such as a reaction solution, even when the vessels (wells) number in excess of 1,000, and even when the dimension, shape, and surface configuration of the vessels (wells) do not permit fluid to flow into the vessels (wells).

Abstract: A method for identifying a microorganism, wherein the method comprises the steps of: 1) preparing one or more kind(s) of double-stranded DNA fragments by the random PCR using at least a part of a genome of an organism of interest, 2) applying the double-stranded DNA fragments which were prepared in step 1 to temperature gradient gel electrophoresis (TGGE) or denaturant gradient gel electrophoresis (DGGE), 3) extracting identification dots of each DNA fragment from the electrophoresis pattern which was obtained in step 2, 4) determining PaSS and/or genome semi-distance from the identification dots which were obtained in step 3, and 5) analyzing the PaSS and/or genome semi-distance which were/was obtained in step 4, wherein pseudo-absolute position of identification dots is determined by the locational relation to standard DNA in the presence of standard DNA as the starting dots in TGGE or DGGE.

Abstract: A method for identifying a microorganism, wherein the method comprises the steps of: 1) preparing one or more kind(s) of double-stranded DNA fragments by the random PCR using at least a part of a genome of an organism of interest, 2) applying the double-stranded DNA fragments which were prepared in step 1 to temperature gradient gel electrophoresis (TGGE) or denaturant gradient gel electrophoresis (DGGE), 3) extracting identification dots of each DNA fragment from the electrophoresis pattern which was obtained in step 2, 4) determining PaSS and/or genome semi-distance from the identification dots which were obtained in step 3, and 5) analyzing the PaSS and/or genome semi-distance which were/was obtained in step 4, wherein pseudo-absolute position of identification dots is determined by the locational relation to standard DNA in the presence of standard DNA as the starting dots in TGGE or DGGE.

Abstract: The present invention provides an improved gate valve system for cutting off a fluid flowing through a pipe. The gate valve system includes a gate valve and at least one member. The gate valve includes a seal member exhibiting an elastically deformable property for sealingly contacting the inner surface of the pipe, and a support member for receiving said seal member and movable in the perpendicular direction to the flowing direction of the fluid between the outside and inside of the pipe for timely bringing said seal member into a sealing contact with the inner surface of the pipe. The at least one expansion member is deformable, but is not substantially subjected to the volume variation even under pressure. The at least one expansion member is embedded in the seal member in such a manner as to expand the seal member laterally outwardly to the inner surface of the pipe upon receiving pressure effected by the sealing contact between the seal member and the inner surface of the pipe.