Abstract: To identify a cytochrome P450 involved in the detoxification and metabolism of a specific growth inhibitor. Provided is a gene encoding a cytochrome P450 classified into Indica rice-derived CYP72A31, comprising a polynucleotide encoding a protein comprising an amino acid sequence of SEQ ID NO: 2.

Abstract: To identify a cytochrome P450 involved in the detoxification and metabolism of a specific growth inhibitor. Provided is a gene encoding a cytochrome P450 classified into Indica rice-derived CYP72A31, comprising a polynucleotide encoding a protein comprising an amino acid sequence of SEQ ID NO: 2.

Abstract: Disclosed is a gene coding for a protein (a) or (b) showing a high level of resistance to PC herbicides or sulfonylurea herbicides. The protein (a) consists of an amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8, and the protein (b) consists of an amino acid sequence derived from the amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8 by substitution, deletion or addition of at least one or more amino acids, has resistance to a pyrimidinyl carboxy herbicide, and has acetolactate synthase activity.

Abstract: Transformed cells are efficiently selected using a mutant ALS gene having high specificity to PC herbicides. The transformation method comprises the steps of: transforming a host cell with a recombination vector containing a gene of interest and a gene coding for a mutant acetolactate synthase having mutation of glycine corresponds to position 95 of the amino acid sequence of a wild-type acetolactate synthase derived from rice to alanine; culturing the transformed cell obtained in the former step in the presence of a pyrimidinyl carboxy herbicide; and wherein the gene coding for the mutant acetolactate synthase is used as a selection marker.

Abstract: The present invention provides a gene coding for the following protein (a) or (b) showing a high level of resistance to PC herbicides or sulfonylurea herbicides: (a) a protein which consists of an amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8; (b) a protein which consists of an amino acid sequence derived from the amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8 by substitution, deletion or addition of at least one or more amino acids, has resistance to a pyrimidinyl carboxy herbicide, and has acetolactate synthase activity.

Abstract: Transformed cells are efficiently selected using a mutant ALS gene having high specificity to PC herbicides. The transformation method comprises the steps of: transforming a host cell with a recombination vector containing a gene of interest and a gene coding for a mutant acetolactate synthase having mutation of glycine corresponds to position 95 of the amino acid sequence of a wild-type acetolactate synthase derived from rice to alanine; culturing the transformed cell obtained in the former step in the presence of a pyrimidinyl carboxy herbicide; and wherein the gene coding for the mutant acetolactate synthase is used as a selection marker.

Abstract: The present invention provides a method for assessing sensitivity of a rice blast fungus to a scytalone dehydratase inhibitor, comprising the steps of: (a) and (b), which (a) identifies an amino acid in an amino acid sequence of a scytalone dehydratase isolated from the rice blast fungus, where the amino acid corresponds to valine at position 75 of SEQ ID NO: 4; and (b) assesses the sensitivity of the rice blast fungus to the scytalone dehydratase inhibitor, where if the amino acid identified in step (a) is methionine, the sensitivity of the rice blast fungus to The scytalone dehydratase inhibitor is assessed to be lower than that of a wild-type rice blast fungus which endogenously produces the scytalone dehydratase of SEQ ID NO: 4.

Abstract: The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi. The gene codes for either one of the following proteins (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2; or (b) a protein consisting of an amino acid sequence shown in SEQ ID NO:2 by deletion substitution or addition of one or more amino acids, which exhibits scytalone dehydratase activity in the presence of a scytalone dehydratase inhibitor.

Abstract: The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi. The gene codes for either one of the following proteins (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2; or (b) a protein consisting of an amino acid sequence shown in SEQ ID NO:2 by deletion, substitution or addition of one or more amino acids, which exhibits scytalone dehydratase activity in the presence of a scytalone dehydratase inhibitor.

Abstract: An isoxazoline derivative represented by the following general formula [I]: wherein R1 and R2 may be the same or different and are each an alkyl group; R3, R4, R5 and R6 are each a hydrogen atom; Y is an optionally substituted 5- to 6-membered aromatic heterocyclic group or fused aromatic heterocyclic group having a hetero atom selected from a nitrogen atom, a oxygen atom and a sulfur atom; and n is an integer: of 0 to 2. The isoxazoline derivative has an excellent herbicidal effect and an excellent selectivity between crop and weed.

Abstract: The present invention provides methods for inducing tissue-specific expression, which includes preparing an expression vector having a promoter. The promoter includes any one of the following DNAs (a) to (c): (a) a DNA, which includes the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which includes the nucleotide sequence ranging from nucleotides 1344 to 2843 of SEQ ID NO: 1; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment including a nucleotide sequence fully complementary to the nucleotide sequence shown in SEQ ID NO: 1, wherein (a), (b) or (c) can control the transcription of a coding sequence located downstream thereof; and wherein the stringent conditions are 5×SSC at 42° C.; The expression vector, also, includes a coding sequence located downstream of the promoter.

Abstract: The present invention provides a gene encoding ALS protein which shows a extremely high level of resistance to pyrimidinyl carboxy herbicides. The gene of the present invention encodes an acetolactate synthase protein, for instance, according to SEQ ID NO:1, where the acetolactate synthase protein has resistance to a pyrimidinyl carboxy herbicide and has acetolactate synthase activity.

Abstract: The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi. The gene codes for either one of the following proteins (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2; or (b) a protein consisting of an amino acid sequence shown in SEQ ID NO:2 by deletion substitution or addition of one or more amino acids, which exhibits scytalone dehydratase activity in the presence of a scytalone dehydratase inhibitor.

Abstract: The present invention provides a gene coding for the following protein (a) or (b) showing a high level of resistance to PC herbicides or sulfonylurea herbicides: (a) a protein which consists of an amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8; (b) a protein which consists of an amino acid sequence derived from the amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8 by substitution, deletion or addition of at least one or more amino acids, has resistance to a pyrimidinyl carboxy herbicide, and has acetolactate synthase activity.

Abstract: The present invention enables characteristic gene expression control and relates to a promoter, which comprises any one of the following DNAs (a) to (c): (a) a DNA, which comprises the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which comprises a nucleotide sequence derived from the nucleotide sequence shown in SEQ ID NO: 1 by deletion, substitution, insertion, or addition of 1 or a plurality of nucleotides, and which can control transcription of a gene located downstream thereof; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment comprising a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 1, and which can control the transcription of a gene located downstream thereof.

Abstract: An isoxazoline derivative represented by the following general formula [I]: 1

Abstract: The present invention provides a gene encoding ALS protein which shows extremely high level of resistance to PC herbicides.

Abstract: The present invention provides a herbicidal composition containing a novel pyrimidine derivative having the formula (1), ##STR1## wherein R.sup.1 is a trifluoromethanesulfonyioxy group, an ethenyl group which may be substituted, or an ethynyl group which may be substituted; R.sup.2 is a hydrogen atom, an alkyl group, 2-trimethylsilyl ethyl group; R.sup.3 and R.sup.4 are the same or different, and are a methoxy group or a halogen atom; X is an oxygen atom or a sulfur atom; and Z is a methine group; or its salt, as an active ingredient.The pyrimidine derivative of the present invention exhibits an excellent herbicidal effect against noxious weeds which grow in paddy fields, upland fields and non-agricultural fields.

Abstract: A herbicidal alkanoic acid derivative of the formula: ##STR1## wherein R.sup.3 is a hydrogen atom, a halogen atom, a halogen-substituted alkyl group, an alkyl group, a cycloalkyl group, an alkylthioalkyl group, a hydroxyalkyl group, a hydroxyl group, a cyano group, an acyloxyalkyl group, a thienyl group, a naphthyl group, a dihydronaphthyl group ##STR2## wherein R.sup.8 is a hydrogen atom, a halogen atom, a nitro group, an alkyl group, an alkoxy group or --S(O).sub.n R.sup.9 wherein R.sup.9 is an alkyl group, and n is an integer of from 0 to 2, m is an integer of from 0 to 2, each of R.sup.2 and R.sup.4 which may be the same or different is a hydrogen atom or an alkyl group, or R.sup.2 and R.sup.4 form together with the adjacent carbon atom a 3-, 4-, 5- or 6-membered ring which may contain an oxygen atom and may be substituted by one or two alkyl groups, each of R.sup.5 and R.sup.6 which may be the same or different is a hydrogen atom or an alkyl group, R.sup.7 is an alkyl group or a phenyl group, or R.sup.

Abstract: A new class of 5H-1,3,4-thiadiazolo[3,2-a]pyrimidin-5-one derivatives were synthesized. They are useful as agricultural-horticultural fungicides.