Abstract: Provided is a microneedle. The microneedle array includes a needle portion, which contains an inactivated whole virion influenza vaccine, and a sheet portion. Also provided is an administration method in which the microneedle array is administered and then administered secondly after an interval of equal to or greater than 24 hours and less than 2 months.

Abstract: Provided is a microneedle. The microneedle array includes a needle portion, which contains an inactivated whole virion influenza vaccine, and a sheet portion. Also provided is an administration method in which the microneedle array is administered and then administered secondly after an interval of equal to or greater than 24 hours and less than 2 months.

Abstract: An object of the present invention is to provide a biosensor comprising a hydrogel capable of immobilizing a physiologically active substance thereon, which can be produced conveniently by use of a safe material, and a method for producing the same. The present invention provides a method for producing a biosensor, which comprises bringing a polymer containing an activated carboxyl group into contact with a substrate surface coated with an organic layer having an amino group to thereby bind the polymer to the organic layer.

Abstract: An object of the present invention is to provide a biosensor comprising a hydrogel capable of immobilizing a physiologically active substance thereon, which can be produced conveniently by use of a safe material, and a method for producing the same. The present invention provides a method for producing a biosensor, which comprises bringing a polymer containing an activated carboxyl group into contact with a substrate surface coated with an organic layer having an amino group to thereby bind the polymer to the organic layer.

Abstract: An object of the present invention is to provide a biosensor that can detect binding of a compound with a functional protein and then assay a reaction product derived from the activity of the functional protein again.

Abstract: It is an object of the present invention to provide a biosensor having excellent preservation stability. The present invention provides a biosensor wherein a first hydrophilic polymer layer is immobilized on a self-assembled membrane applied onto the surface of a metal substrate, a second hydrophilic polymer layer is applied onto the first hydrophilic polymer layer, and the total film thickness of the first hydrophilic polymer layer and the second hydrophilic polymer layer in a dry state is between 20 nm and 100 nm.

Abstract: A sensor unit of a surface plasmon resonance (SPR) assay system includes a transparent dielectric medium. A thin film has a first surface and a sensing surface. The first surface is connected with the dielectric medium to constitute an interface. The sensing surface is back to the first surface, for detecting (bio) chemical reaction. A flow cell block has a flow channel for flowing of the sample to the sensing surface. Attenuated total reflection of illuminating light is checked at the interface, to analyze interaction between ligand and analyte as samples. The flow channel includes a first inner surface, disposed opposite to the sensing surface to extend along, for passing the sample to flow between. The first inner surface has a height, defined with reference to the sensing surface, and in a range of 200-500 microns.

Abstract: A flow cell device is incorporated in the sensor unit for surface plasmon resonance (SPR) assay. A box shaped flow cell body has a lower surface, and is disposed in contact of the lower surface with a sensing surface for detecting reaction of sample. Two flow channels are formed in the flow cell body, for flow of the sample. Each of the flow channels include a fluid passageway, formed in the lower surface, for flow of the sample in contact with the sensing surface. Two end cavities are formed in an upper surface of the flow cell body and open therein, to extend from ends of the fluid passageway. The flow channels are arranged adjacent to one another, disposed to extend in a direction of the flow. Fluid passageways of the flow channels are overlapped partially on one another in a flow cell longitudinal direction of the flow cell body.

Abstract: An object to be solved by the present invention is to determine the dissociation constant of an analyte molecule immobilized on a metal surface and a molecule that interacts therewith in surface plasmon resonance (SPR) analysis via an analytical method that produces a low noise level (i.e., a noise width of a reference chip), small baseline fluctuations (i.e., signal changes of a reference chip), and highly reliable results of measurement.

Abstract: A sensor unit of a surface plasmon resonance (SPR) assay system includes a transparent dielectric medium. A thin film has a first surface and a sensing surface. The first surface is connected with the dielectric medium to constitute an interface. The sensing surface is back to the first surface, for detecting (bio) chemical reaction. A flow cell block has a flow channel for flowing of the sample to the sensing surface. Attenuated total reflection of illuminating light is checked at the interface, to analyze interaction between ligand and analyte as samples. The flow channel includes a first inner surface, disposed opposite to the sensing surface to extend along, for passing the sample to flow between. The first inner surface has a height, defined with reference to the sensing surface, and in a range of 200-500 microns.

Abstract: It is an object of the present invention to provide a method for carrying out on a same biosensor both of the measurement of the amount of a physiologically active substance immobilized on a biosensor substrate and the measurement of the biological activity of the above physiologically active substance, thereby analyzing the interaction of a substance with the above physiologically active substance that maintains its activity. The present invention provides a measurement method using a biosensor substrate, wherein both the amount of a physiologically active substance immobilized on the substrate and the biological activity of the physiologically active substance immobilized on the substrate are measured on said same biosensor substrate.

Abstract: A sensor unit of a surface plasmon resonance (SPR) assay system includes a transparent dielectric medium. A thin film has a first surface and a sensing surface. The first surface is connected with the dielectric medium to constitute an interface. The sensing surface is back to the first surface, for detecting (bio)chemical reaction. A flow cell block has a flow channel for flowing of the sample to the sensing surface. Attenuated total reflection of illuminating light is checked at the interface, to analyze interaction between ligand and analyte as samples. The flow channel includes a first inner surface, disposed opposite to the sensing surface to extend along, for passing the sample to flow between. The first inner surface has a height, defined with reference to the sensing surface, and in a range of 200-500 microns.

Abstract: The present invention provides a method for preparing an immobilization substrate for a biosensor, the immobilization substrate having a measurement surface having a physiologically active substance immobilized thereon, and a reference surface not having a physiologically active substance immobilized thereon, the biosensor measuring an interaction, in terms of a change in refractive index, between the physiologically active substance and a test substance in a solution which is supplied to both the measurement surface and the reference surface, the method comprising: performing an activation treatment on a part of the substrate surface by using an organic solvent and an activating agent, thereby forming, on the substrate, a first surface which is activated such that it can immobilize the physiologically active substance, and forming a second surface which has not been subjected to the activation treatment; forming a flow channel which includes both the first surface and the second surface by attaching a flow c

Abstract: An object of the present invention is to provide a biosensor that can detect binding of a compound with a functional protein and then assay a reaction product derived from the activity of the functional protein again.

Abstract: It is an object of the present invention to provide a method for measuring the interaction between a physiologically active substance immobilized on the surface of a substrate and a test substance, wherein the influence of negative signals intermittently generated due to desorption of the physiologically active substance as a ligand from the surface of the substrate upon the measured value that indicates the binding amount of a test substance as an analyte can be eliminated. The present invention provides a method for measuring the interaction between a physiologically active substance immobilized on the surface of a substrate and a test substance, wherein a calibration curve is produced by using baseline values obtained by repeatedly measuring a single type of solution, and the measurement value of said test substance is calibrated by said calibration curve, so as to obtain the interaction signals of said test substance.

Abstract: An object of the present invention is to suppress the noise width of a reference cell during measurement and the base line fluctuation. The present invention provides a method for measuring a change in surface plasmon resonance, which comprises: using a surface plasmon resonance measurement device comprising a flow channel system having a cell formed on a metal film and a light-detecting means for detecting the state of surface plasmon resonance by measuring the intensity of a light beam totally reflected on the meal film; and exchanging the liquid contained in the above flow channel system, wherein the above method is characterized in that a change in surface plasmon resonance is measured in a state where the flow of the liquid has been stopped, after the liquid contained in the above flow channel system has been exchanged.

Abstract: An object of the present invention is to provide a method for production of a physiologically active substance-immobilized substrate, which can prevent the inactivation of a physiologically active substance and/or can immobilize a physiologically active substance at a high concentration onto a substrate surface without use of electrostatic attraction. The present invention provides a method for production of a physiologically active substance-immobilized substrate, which comprises steps of: applying, to a metal substrate having a layer for immobilizing a physiologically active substance, a solution containing a physiologically active substance capable of forming a covalent bond with a molecule constituting the layer for immobilizing a physiologically active substance; and then drying the solution.

Abstract: A method for screening a specified compound, the method comprising: repeatedly applying an assay/washing process to the same biologically active substance for each of different plural compounds, the assay/washing process comprising supplying a compound to an attached biologically active substance, assaying the amount of binding between the biologically active substance and the compound, and washing the compound supplied to the biologically active substance after the assay; and repeating an activity assay for detecting the activity of the biologically active substance at least twice during the interval from before starting of the first assay/washing process to after completion of the last assay/washing process, wherein a relationship of activity change indicating the activity change of the biologically active substance is determined based on the activity of the biologically active substance obtained by the activity assay, a corrected amount of binding is determined by correcting the amount of binding between t

Abstract: An object of the present invention is to provide a method for preventing the generation of negative signals because of dissociation of a ligand from a surface and improving the precision of signals indicating binding between a ligand and an analyte. The present invention provides a method for immobilizing a biomolecule on a carrier having reactive groups which comprises: (i) a step of activating some reactive groups so that they can form a covalent bond with a biomolecule; (ii) a step of reacting the biomolecule with the aforementioned activated reactive groups; and (iii) a step of again activating some reactive groups; wherein the steps (i), (ii) and (iii) are performed in this order.

Abstract: An object to be solved by the present invention is to determine the dissociation constant of an analyte molecule immobilized on a metal surface and a molecule that interacts therewith in surface plasmon resonance (SPR) analysis via an analytical method that produces a low noise level (i.e., a noise width of a reference chip), small baseline fluctuations (i.e., signal changes of a reference chip), and highly reliable results of measurement.