Abstract: The present invention provides for a plasmid, pEAP7.DELTA.P, containing a DNA region capable of inducing extracellular secretion of a useful, physiologically active substance in transformed host and a promoter DNA region regulating expression of the first DNA region. The present invention further provides for a microorganism transformed with the plasmid and a process for the production of the substances by culturing the microorganism.

Abstract: A novel plasmid pXP102-3 which is constructed from Aeromonas sp. No. 212 chromosomal DNA fragment coding for production of xylanase and a vector plasmid pEAP2 carrying a DNA fragment coding for production and secretion of penicillinase. A novel microorganism, Escherichia coli HB101 (pXP102-3), containing the plasmid pXP102-3 and being capable of producing and secreting penicillinase and xylanase. A method for cultivation of the microorganism characterized in that it is cultured in a broth containing NaCl for 12 to 48 hours.

Abstract: Methanosarcina alcaliphilum which is alkalophilic methanogen having the optimum growth pH range of from about 8.1 to about 8.7. This strain exhibits excellent properties such as alkalophilicity and resistance to low temperature. This strain makes it possible to carry out methane fermentation methods at an alkaline pH and at a low temperature conditions. This strain is applied to methane fermentation in treating solid waste and waste water. Moreover, the bacterial concentration thereof in a reactor can be increased within a very short time and, therefore, the size of such a reactor can be made compact.

Abstract: Methanohalococcus alcaliphilum having an optimum growth sodium chloride concentration ranging from about 2.5 to about 3 mole are herein disclosed. The bacteria are halophilic or halophilic and alkalophilic and can be used as the microorganisms which play a central role in the methods of methane fermentation to enhance the efficiency of such a method since the use of the microorganism makes it possible to carry out the method at an alkaline pH, in the presence of salts in a high concentration and at a low temperature.

Abstract: A novel plasmid pEAP2, which was constructed from Bacillus sp. 170 chromosomal DNA carrying the gene for extracellular production of penicillinase and a vector plasmid pMB9. A novel microorganism, Escherichia coli HB101(pEAP2) carrying the plasmid pEAP2 and being capable of extracellular production of penicillinase. The method for cultivation of the microorganism is characterized by culturing it in the medium containing NaCl (or KCl) for 16-48 hours. According to this invention, useful high-molecular substances can be produced in a high yield.

Abstract: The present invention relates to a method for preparing magnetic powder comprising homogeneous and fine particles using an alkali-producing enzyme. The object of the present invention is to provide a method suitable for preparing magnetic powder comprising relatively small particles, for instance, fine particles having a particle size ranging from 50 to 500 nm. The present invention relates to a method for preparing at least one member selected from the group consisting of iron oxides, iron hydroxides and iron oxyhydroxides which comprises the step of alkalizing a solution containing iron ions utilizing an alkali-producing enzyme and a substrate of the enzyme.According to the present invention, there can be produced magnetite (Fe.sub.3 O.sub.4) and maghemite (gamma-Fe.sub.2 O.sub.3) useful as magnetic powder as well as goethite (alpha-FeO(OH)), hematite (alpha-Fe.sub.2 O.sub.3) and lepidocrocite (gamma-FeO(OH)) useful as the starting materials thereof.

Abstract: This invention relates to a compound, FR-900848, having antimicrobial activity against various microorganisms, to a process for the preparation of same and to a pharmaceutical composition comprising same.

Abstract: A crosslinking agent constituted by a cyclodextrin clathrate compound of a compound having isocyanate group(s). This crosslinking agent is stable at room temperature and very readily initiates crosslinking. The crosslinking agent is prepared by reacting a compound having isocyanate group(s) with cyclodextrin at 65.degree. C. or below in water, and recovering the clathrate compound from the water.

Abstract: A novel plasmid pCX311, which was constructed from Bacillus sp. C125 chromosomal DNA carrying the gene for extracellular production of xylanase and a vector plasmid pBR322. A novel microorganism, Escherichia coli HB101 (pCX311) carrying the plasmid pCX311 and being capable of extracellular production of xylanase. Method for culturing the microorganism characterized by cultivation of it in the medium containing NaCl (or KCl) and bran, or NaCl (or KCl) and xylan for 12-48 hours. According to this invention, useful high-molecular substances can be produced in a high yield.

Abstract: Process for the oil extraction from oil sand comprising: mixing oil sand, cyclodextrin and water with one another to prepare a suspension, leaving it to stand or centrifuging it to separate into an oil, a water and a sand layers, and then collecting the oil layer.

Abstract: Process for the oil extraction from oil sand comprising: mixing oil sand, cyclodextrin, a hydrocarbon solvent, a flocculating agent and water with one another to prepare a suspension, leaving the suspension to stand or centrifuging it to separate into an oil, a water and a sand layer, and then collecting the oil layer.

Abstract: This invention relates to a process for producing .gamma.-cyclodextrin without using any organic solvents i.e. by non-solvent process and more particularly, to processes for producing .gamma.-cyclodextrin, which comprise: (1) passing a sugar solution containing cyclodextrins and reducing sugars as primary ingredients through a column packed with alkali or alkali earth metal salts of strongly acidic cation exchange resin to separate cyclodextrins fraction from reducing sugars fraction and then, passing the cyclodextrins fraction through a column packed with gel resin particles to separate and collect .gamma.-cyclodextrin and, (2) bringing the sugar solution into contact with a hydrophobic, synthetic adsorption resin comprising a porous polymer to adsorb only .gamma.-cyclodextrin or .gamma.- and .beta.-cyclodextrins and then, eluting the adsorbed cyclodextrin(s) with water to separate and collect .gamma.-cyclodextrin.

Abstract: The present invention relates to a novel process for recovering cyclodextrins, characterized in that a solution containing cyclodextrins as well as starches, dextrins, reducing sugars and the like is brought into contact with a hydrophobic, synthetic adsorption resin comprising a porous polymer to adsorb only cyclodextrins, the cyclodextrins thus adsorbed being eluted thereafter with water while being heated to separate and recover said cyclodextrins.

Abstract: This invention provides a novel process for producing cyclodextrin by reacting cyclodextrin glycosyl transferase having an optimum pH on the alkaline side with gelatinized starch. According to this invention, cyclodextrin can be obtained in the form of a pure crystal in a high yield by reacting a glucomylase with the reaction mixture liquid formed by the above enzymatic reaction to decompose unreacted starch, concentrating the liquid, adding a small amount of cyclodextrin as a seed crystal and recovering the precipitated cyclodextrin.

Abstract: An alkaline protease is produced by cultivation of novel microorganism strains belonging to the Bacillus genus. The alkaline protease has optimum pH of 11 to 12 and is useful as an additive for detergents.

Abstract: A new deoxyribonuclease (DNase) having preservability and high temperature stability is provided by cultivating a new strain M-26 of the genus Bacillus in an alkaline culture medium. The DNase has such a specificity that it very selectively cleaves phosphodiester bonds between deoxyguanosine and deoxyguanosine in a molecule of deoxyribonucleic acid (DNA) while leaving phosphate groups at the 5'-position.