Patents by Inventor Kole T. Roybal
Kole T. Roybal has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240075062Abstract: The present disclosure generally relates to, inter alia, a new class of receptors engineered to modulate transcriptional regulation in a ligand-dependent manner. Particularly, the new receptors, even though derived from Notch, do not require the Notch negative regulatory regions previously believed to be essential for the functioning of the receptors. In addition, the new receptors described herein incorporate an extracellular oligomerization domain to promote oligomer formation of the chimeric receptors. The disclosure also provides compositions and methods useful for producing such receptors, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various health conditions such as cancers.Type: ApplicationFiled: March 3, 2023Publication date: March 7, 2024Inventors: Kole T. ROYBAL, Raymond LIU, Iowis ZHU
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Patent number: 11897932Abstract: The present disclosure generally relates to, among other things, a new class of receptors engineered to modulate transcriptional regulation in a ligand-dependent manner. Particularly, the new receptors, even though derived from Notch, do not require the Notch negative regulatory regions previously believed to be essential for the functioning of the receptors. The disclosure also provides compositions and methods useful for producing such receptors, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various health conditions or diseases, such as cancers.Type: GrantFiled: March 30, 2021Date of Patent: February 13, 2024Assignee: The Regents of the University of CaliforniaInventors: Kole T. Roybal, Raymond Liu, Iowis Zhu
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Publication number: 20230312673Abstract: This disclosure relates to chimeric receptors (CRs) and their signaling components for the regulation of an immune response. Also provided are nucleic acids encoding the disclosed CRs, recombinant immune cells expressing the same, and pharmaceutical compositions containing the disclosed nucleic acids and/or recombinant cells.Type: ApplicationFiled: September 1, 2021Publication date: October 5, 2023Inventors: Kole T. ROYBAL, Daniel B. GOODMAN, Camillia AZIMI, Alexander MARSON, Jeffrey A. BLUESTONE
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Publication number: 20230212557Abstract: The present disclosure provides synthetic modular polypeptide libraries and nucleic acids encoding such synthetic modular polypeptide libraries. Also provided are methods of making synthetic modular polypeptide libraries and nucleic acids encoding synthetic modular polypeptide libraries. Methods of screening a synthetic modular polypeptide library to identify a selected phenotype associated with a member of a synthetic modular polypeptide library are also provided where such methods fmd use in both in vitro and in vivo assays.Type: ApplicationFiled: December 19, 2022Publication date: July 6, 2023Inventors: Wendell A. Lim, Scott M. Coyle, Russell M. Gordley, Kole T. Roybal
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Publication number: 20230183709Abstract: The present disclosure generally relates to, inter alia, a new class of chimeric Notch receptors containing a fully humanized transcriptional effector, engineered to modulate gene expression and cellular activities in a ligand-dependent manner. The new chimeric Notch receptors surprisingly retain the ability to transduce signals in response to ligand binding despite that the Notch extracellular subunit (NEC), which includes the negative regulatory region (NRR) previously believed to be essential for the functioning of Notch receptors is completely absent. In addition, the new receptors described herein incorporate an extracellular oligomerization domain to promote oligomer formation of the chimeric receptors. Also provided are compositions and methods useful for producing such receptors, nucleic acids encoding same, engineered cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various diseases such as cancers.Type: ApplicationFiled: April 8, 2021Publication date: June 15, 2023Inventors: Kole T. ROYBAL, Raymond LIU
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Publication number: 20230174612Abstract: The present disclosure generally relates to, inter alia, a new class of chimeric Notch receptors containing a synthetic zinc finger transcriptional effector (synZTE) module, engineered to modulate gene expression and cellular activities in a ligand-dependent manner. The new Notch receptors surprisingly retain the ability to transduce signals in response to ligand binding despite that the Notch extracellular subunit (NEC), which includes the negative regulatory region (NRR) previously believed to be essential for the functioning of Notch receptors, is partly or completely deleted. In addition, the synZTE is designed to bind orthogonal DNA target sequences in target organisms which in turn facilitates precise regulation of therapeutic gene expression with minimal off-target activity.Type: ApplicationFiled: March 24, 2021Publication date: June 8, 2023Inventors: Kole T. ROYBAL, Iowis ZHU, Raymond LIU, Ahmad S. KHALIL, Divya ISRANI
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Patent number: 11617766Abstract: The present disclosure generally relates to, inter alia, a new class of receptors engineered to modulate transcriptional regulation in a ligand-dependent manner. Particularly, the new receptors, even though derived from Notch, do not require the Notch negative regulatory regions previously believed to be essential for the functioning of the receptors. In addition, the new receptors described herein incorporate an extracellular oligomerization domain to promote oligomer formation of the chimeric receptors. The disclosure also provides compositions and methods useful for producing such receptors, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various health conditions such as cancers.Type: GrantFiled: November 17, 2021Date of Patent: April 4, 2023Assignee: The Regents of the University of CaliforniaInventors: Kole T. Roybal, Raymond Liu, Iowis Zhu
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Patent number: 11560561Abstract: The present disclosure provides synthetic modular polypeptide libraries and nucleic acids encoding such synthetic modular polypeptide libraries. Also provided are methods of making synthetic modular polypeptide libraries and nucleic acids encoding synthetic modular polypeptide libraries. Methods of screening a synthetic modular polypeptide library to identify a selected phenotype associated with a member of a synthetic modular polypeptide library are also provided where such methods find use in both in vitro and in vivo assays.Type: GrantFiled: August 31, 2016Date of Patent: January 24, 2023Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Wendell A. Lim, Scott M. Coyle, Russell M. Gordley, Kole T. Roybal
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Publication number: 20220378832Abstract: A method for treating a subject for glioblastoma is provided. In some embodiments, the method may comprise administering to a subject a molecular circuit that includes a binding triggered transcriptional switch (BTTS) that, when bound to MOG, induces one or more encoded therapeutics specific for one or more antigens expressed by the GBM. Nucleic acids containing sequences encoding all or portions of such circuits are also provided, as well as cells, expression cassettes and vectors that contain such nucleic acids. Also provided are kits for practicing the described methods.Type: ApplicationFiled: November 6, 2020Publication date: December 1, 2022Inventors: Wendell A. Lim, Milos Simic, Hideho Okada, Kole T. Roybal, Joseph H. Choe, Payal B. Watchmaker
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Publication number: 20220372101Abstract: The present disclosure generally relates to, among other things, a new class of receptors engineered to modulate transcription in a ligand-dependent manner. The new receptors provide a selectable degree of noise, expression level, and signal to noise ratio. The disclosure also provides compositions and methods useful for producing such receptors, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various health conditions or diseases, such as cancers.Type: ApplicationFiled: September 23, 2020Publication date: November 24, 2022Inventors: Kole T. ROYBAL, Raymond LIU, Iowis ZHU
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Publication number: 20220356225Abstract: The present disclosure generally relates to, inter alia, a new class of chimeric Notch receptors containing a synthetic zinc finger transcriptional effector (synZTE) module, engineered to modulate gene expression and cellular activities in a ligand-dependent manner. The new Notch receptors surprisingly retain the ability to transduce signals in response to ligand binding despite that the Notch extracellular subunit, which includes the negative regulatory region previously believed to be essential for the functioning of Notch receptors, is partly or completely deleted. In addition, the synZTE is designed to bind orthogonal DNA target sequences in target organisms which in turn facilitates precise regulation of therapeutic gene expression with minimal off-target activity.Type: ApplicationFiled: September 23, 2020Publication date: November 10, 2022Inventors: Kole T. ROYBAL, Iowis ZHU, Raymond LIU, Ahmad S. KHALIL, Divya ISRANI
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Publication number: 20220348677Abstract: The present disclosure generally relates to, among other things, a new class of receptors engineered to modulate transcriptional regulation in a ligand-dependent manner. In particular, the new receptors contain a heterologous transmembrane domain comprising at least one ?-secretase site. The disclosure also provides compositions and methods useful for producing such receptors, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various health conditions or diseases, such as cancers.Type: ApplicationFiled: September 23, 2020Publication date: November 3, 2022Inventors: Kole T. ROYBAL, Raymond LIU, Iowis ZHU
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Publication number: 20220348628Abstract: The present disclosure generally relates to, among other things, a new class of receptors engineered to modulate transcriptional regulation in a ligand-dependent manner Particularly, the new receptors, even though derived from Notch and Robo, do not require the Notch or Robo regulatory regions previously believed to be necessary for the functioning of the receptors. The disclosure also provides compositions and methods useful for producing such receptors, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various health conditions such as diseases (e.g., cancers).Type: ApplicationFiled: September 23, 2020Publication date: November 3, 2022Inventors: Kole T. ROYBAL, Raymond LIU, Iowis ZHU
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Publication number: 20220340637Abstract: The present disclosure generally relates to, inter alia, a new class of receptors engineered to modulate transcriptional regulation in a ligand-dependent manner. Particularly, the new receptors, even though derived from Notch, do not require the Notch negative regulatory regions previously believed to be essential for the functioning of the receptors. In addition, the new receptors described herein incorporate a flexible polypeptide connector region disposed between the extracellular ligand-binding domain (ECD) and the transmembrane domain (TMD) which facilitates optimizing the stiffness/flexibility of the chimeric polypeptides to achieve a desired activity. The disclosure also provides compositions and methods useful for producing such receptors, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various health conditions such as diseases (e.g., cancers).Type: ApplicationFiled: September 23, 2020Publication date: October 27, 2022Inventors: Kole T. ROYBAL, Raymond LIU, Iowis ZHU
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Publication number: 20220324982Abstract: The instant disclosure provides chimeric polypeptides which modulate various cellular processes following a cleavage event induced upon binding of a specific binding member of the polypeptide with its binding partner. Methods of using chimeric polypeptides to modulate cellular functions, including e.g., induction of gene expression, are also provided. Nucleic acids encoding the subject chimeric polypeptides and associated expression cassettes and vectors as well as cells that contain such nucleic acids and/or expression cassettes and vectors are provided. Also provided, are methods of treating a subject using the described components and methods as well as kits for practicing the subject methods.Type: ApplicationFiled: June 15, 2022Publication date: October 13, 2022Inventors: Wendell A. Lim, Kole T. Roybal, Jasper Z. Williams
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Patent number: 11401332Abstract: The instant disclosure provides chimeric polypeptides which modulate various cellular processes following a cleavage event induced upon binding of a specific binding member of the polypeptide with its binding partner. Methods of using chimeric polypeptides to modulate cellular functions, including e.g., induction of gene expression, are also provided. Nucleic acids encoding the subject chimeric polypeptides and associated expression cassettes and vectors as well as cells that contain such nucleic acids and/or expression cassettes and vectors are provided. Also provided, are methods of treating a subject using the described components and methods as well as kits for practicing the subject methods.Type: GrantFiled: August 22, 2017Date of Patent: August 2, 2022Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Wendell A. Lim, Kole T. Roybal, Jasper Z. Williams
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Publication number: 20220133797Abstract: The present disclosure generally relates to, inter alia, a new class of receptors engineered to modulate transcriptional regulation in a ligand-dependent manner. Particularly, the new receptors, even though derived from Notch, do not require the Notch negative regulatory regions previously believed to be essential for the functioning of the receptors. In addition, the new receptors described herein incorporate an extracellular oligomerization domain to promote oligomer formation of the chimeric receptors. The disclosure also provides compositions and methods useful for producing such receptors, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various health conditions such as cancers.Type: ApplicationFiled: November 17, 2021Publication date: May 5, 2022Inventors: Kole T. Roybal, Raymond Liu, Iowis Zhu
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Patent number: 11202801Abstract: The present disclosure generally relates to, inter alia, a new class of receptors engineered to modulate transcriptional regulation in a ligand-dependent manner. Particularly, the new receptors, even though derived from Notch, do not require the Notch negative regulatory regions previously believed to be essential for the functioning of the receptors. In addition, the new receptors described herein incorporate an extracellular oligomerization domain to promote oligomer formation of the chimeric receptors. The disclosure also provides compositions and methods useful for producing such receptors, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various health conditions such as cancers.Type: GrantFiled: March 30, 2021Date of Patent: December 21, 2021Assignee: The Regents of the University of California, UCSFInventors: Kole T. Roybal, Raymond Liu, Iowis Zhu
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Publication number: 20210268024Abstract: The present disclosure generally relates to, inter alia, a new class of receptors engineered to modulate transcriptional regulation in a ligand-dependent manner. Particularly, the new receptors, even though derived from Notch, do not require the Notch negative regulatory regions previously believed to be essential for the functioning of the receptors. In addition, the new receptors described herein incorporate an extracellular oligomerization domain to promote oligomer formation of the chimeric receptors. The disclosure also provides compositions and methods useful for producing such receptors, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various health conditions such as cancers.Type: ApplicationFiled: March 30, 2021Publication date: September 2, 2021Inventors: Kole T. ROYBAL, Raymond Liu, Iowis Zhu
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Publication number: 20210246186Abstract: The present disclosure generally relates to, among other things, a new class of receptors engineered to modulate transcriptional regulation in a ligand-dependent manner. Particularly, the new receptors, even though derived from Notch, do not require the Notch negative regulatory regions previously believed to be essential for the functioning of the receptors. The disclosure also provides compositions and methods useful for producing such receptors, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various health conditions or diseases, such as cancers.Type: ApplicationFiled: March 30, 2021Publication date: August 12, 2021Inventors: Kole T. Roybal, Raymond Liu, Iowis Zhu