Patents by Inventor Konan Peck

Konan Peck has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9891227
    Abstract: Methods of assaying a biological target are disclosed. The method comprises: (a) providing a sample containing the biological target; (b) providing biotin-labeled first aptamers conjugated to a gold nanoparticle (GNP), and second aptamers conjugated to a magnetic bead, wherein the first and the second aptamers exhibit specific binding affinities to the target; (c) incubating the sample with the first and the second aptamers to obtain target-bound aptamers; (d) separating the target-bound aptamers from unbound aptamers; (e) eluting the first aptamers from the GNP; (f) incubating the eluted biotin-labeled first aptamers with streptavidin-magnetic beads and reporter gold nanoparticles (GNPs) to obtain a complex comprising: the bead, the first aptamers, attached to the bead; and the reporter GNPs captured by the bead through the first aptamers; (g) eluting the reporter GNPs captured; and (h) detecting the target by measuring and analyzing a light-scattering signal of the eluted reporter GNPs.
    Type: Grant
    Filed: April 30, 2015
    Date of Patent: February 13, 2018
    Assignee: ACADEMIA SINICA
    Inventors: Konan Peck, Pan-Chyr Yang, Yi-Chung Chang
  • Patent number: 9856480
    Abstract: The invention provides DNAzymes which are capable to silence the expression of EGFR at allele-specific level. These allele-specific DNAzymes against EGFR T790M mutation will knockdown the expression of EGFR T790M mRNA while keeping EGFR wild-type mRNA intact. Hence, these allele-specific DNAzymes against EGFR T790M mutation may overcome T790M-derived TKI resistance accompanied with lower unwanted side effects on normal cells in lung cancer patients.
    Type: Grant
    Filed: January 14, 2014
    Date of Patent: January 2, 2018
    Assignees: National Taiwan University, ACADEMIA SINICA
    Inventors: Pan-Chyr Yang, Wei-Yun Lai, Konan Peck, Cheng-Ju Chang, Chi-Yuan Chen, Shuenn-Chen Yang
  • Publication number: 20170211067
    Abstract: Aptamers that bind to and inhibit CTLA-4 and uses thereof in enhancing immune activities, and treating cancer and HIV infection are provided.
    Type: Application
    Filed: July 31, 2015
    Publication date: July 27, 2017
    Applicants: Academia Sinica, National Taiwan University, Taipei Medical University
    Inventors: Konan Peck, Pan-Chyr Yang, Yi-Chung Chang, Bo-Tsang Huang, Shauh-Der Yeh
  • Publication number: 20160145625
    Abstract: The invention provides DNAzymes which are capable to silence the expression of EGFR at allele-specific level. These allele-specific DNAzymes against EGFR T790M mutation will knockdown the expression of EGFR T790M mRNA while keeping EGFR wild-type mRNA intact. Hence, these allele-specific DNAzymes against EGFR T790M mutation may overcome T790M-derived TKI resistance accompanied with lower unwanted side effects on normal cells in lung cancer patients.
    Type: Application
    Filed: January 14, 2014
    Publication date: May 26, 2016
    Applicant: Academia Sinica
    Inventors: Pan-Chyr Yang, Wei-Yun Lai, Konan Peck
  • Publication number: 20150316545
    Abstract: Methods of assaying a biological target are disclosed. The method comprises: (a) providing a sample containing the biological target; (b) providing biotin-labeled first aptamers conjugated to a gold nanoparticle (GNP), and second aptamers conjugated to a magnetic bead, wherein the first and the second aptamers exhibit specific binding affinities to the target; (c) incubating the sample with the first and the second aptamers to obtain target-bound aptamers; (d) separating the target-bound aptamers from unbound aptamers; (e) eluting the first aptamers from the GNP; (f) incubating the eluted biotin-labeled first aptamers with streptavidin-magnetic beads and reporter gold nanoparticles (GNPs) to obtain a complex comprising: the bead, the first aptamers, attached to the bead; and the reporter GNPs captured by the bead through the first aptamers; (g) eluting the reporter GNPs captured; and (h) detecting the target by measuring and analyzing a light-scattering signal of the eluted reporter GNPs.
    Type: Application
    Filed: April 30, 2015
    Publication date: November 5, 2015
    Inventors: Konan PECK, Pan-Chyr Yang, Yi-Chung Chang
  • Publication number: 20090220985
    Abstract: The present invention discloses a method for rapid assessment of lung cancer therapy efficacy in a few days instead of weeks by conventional imaging methods. This method can also be used to detect relapse of the cancer and to improve the current TNM cancer staging method for more accurate prognosis. The rapid assessment of therapy efficacy is based on detecting circulating cancer cells in body fluid with high positive detection rate. The high positive detection rate is achieved by using qPCR amplification of multiple marker genes identified by in silico search of DNA sequence database. This invention also discloses a scoring method to calculate the cancer cell load based on qPCR results to correlate the amount of circulating cancer cells in lung cancer patients and predict the treatment outcomes.
    Type: Application
    Filed: March 24, 2009
    Publication date: September 3, 2009
    Applicant: National Health Research Institutes
    Inventors: Konan Peck, Yuh-Pyng Sher, Jin-Yuan Shih, Pan-Chyr Yang, Cheng-Wen Wu
  • Patent number: 7507534
    Abstract: The present invention discloses a method for rapid assessment of lung cancer therapy efficacy in a few days instead of weeks by conventional imaging methods. This method can also be used to detect relapse of the cancer and to improve the current TNM cancer staging method for more accurate prognosis. The rapid assessment of therapy efficacy is based on detecting circulating cancer cells in body fluid with high positive detection rate. The high positive detection rate is achieved by using qPCR amplification of multiple marker genes identified by in silico search of DNA sequence database. This invention also discloses a scoring method to calculate the cancer cell load based on qPCR results to correlate the amount of circulating cancer cells in lung cancer patients and predict the treatment outcomes.
    Type: Grant
    Filed: December 31, 2005
    Date of Patent: March 24, 2009
    Assignee: National Health Research Institutes
    Inventors: Konan Peck, Yuh-Pyng Sher, Jin-Yuan Shih, Pan-Chyr Yang, Cheng-Wen Wu
  • Patent number: 7354709
    Abstract: This invention is based on the discovery of an association of Collapsin Response Mediator Protein-1 (CRMP-1) with tumor metastasis. The level of CRMP-1 protein or mRNA can be used as an indicator of cellular invasiveness and of a test compound's ability to alter cellular invasiveness. The level of CRMP-1 protein can also be altered, e.g., to reduce invasiveness.
    Type: Grant
    Filed: June 25, 2002
    Date of Patent: April 8, 2008
    Assignees: National Taiwan University, Academia Sinica, National Health Research Institutes
    Inventors: Pan-Chyr Yang, Jin-Yuan Shih, Jeremy J. W. Chen, Konan Peck, Cheng-Wen Wu, Tse-Ming Hong, Shuenn-Chen Yang
  • Publication number: 20070048750
    Abstract: The present invention discloses a method for rapid assessment of lung cancer therapy efficacy in a few days instead of weeks by conventional imaging methods. This method can also be used to detect relapse of the cancer and to improve the current TNM cancer staging method for more accurate prognosis. The rapid assessment of therapy efficacy is based on detecting circulating cancer cells in body fluid with high positive detection rate. The high positive detection rate is achieved by using PCR amplification of multiple marker genes identified by in silico search of DNA sequence database. This invention also discloses a scoring method to calculate the cancer cell load based on PCR results to correlate the amount of circulating cancer cells in lung cancer patients with their treatment outcomes.
    Type: Application
    Filed: December 31, 2005
    Publication date: March 1, 2007
    Applicant: NATIONAL HEALTH RESEARCH INSTITUTE
    Inventors: Konan Peck, Yuh-Pyng Sher, Jin-Yuan Shih, Pan-Chyr Yang, Cheng-Wen Wu
  • Patent number: 6867050
    Abstract: Apparatuses and methods are described for parallel oligonucleotide synthesis of hundreds of different sequences and lengths at a time. Standard phosphoramidite chemistry is employed. The syntheses take place in a reaction plate compatible with the industrial standard microplate format to allow the use of readily available automated instruments for subsequent processing. Key parameters in reducing synthesis volume in small reaction wells are discussed. This invention provides solutions to the difficulties of low volume, high number synthetic reactions.
    Type: Grant
    Filed: August 6, 2001
    Date of Patent: March 15, 2005
    Assignee: Academia Sinica
    Inventors: Konan Peck, Ji-Yen Cheng
  • Publication number: 20030207270
    Abstract: The present invention provides the tools and methodologies necessary to guide the standardization of herbal compositions, to determine which specific components of an herbal composition are responsible for any particular biological activity, to predict the biological activities of a particular herbal composition, to determine the relatedness of herbal compositions, and for the development of improved herbal therapeutics. This invention provides the tools and methodologies for creating, maintaining, improving and utilizing Herbal BioResponse Arrays (HBR Arrays), wherein the HBR Arrays constitute data sets associated with particular herbal compositions.
    Type: Application
    Filed: August 2, 2002
    Publication date: November 6, 2003
    Inventors: Patrick C. Kung, Konan Peck, Yun-Shien Lee, Yuh-Pyng Sher, Yung-Chi Cheng
  • Publication number: 20030077624
    Abstract: This invention is based on the discovery of an association of Collapsin Response Mediator Protein-1 (CRMP-1) with tumor metastasis. The level of CRMP-1 protein or mRNA can be used as an indicator of cellular invasiveness and of a test compound's ability to alter cellular invasiveness. The level of CRMP-1 protein can also be altered, e.g., to reduce invasiveness.
    Type: Application
    Filed: June 25, 2002
    Publication date: April 24, 2003
    Inventors: Pan-Chyr Yang, Jin-Yuan Shih, Jeremy J. W. Chen, Konan Peck, Cheng-Wen Wu, Tse-Ming Hong, Shuenn-Chen Yang
  • Publication number: 20030054387
    Abstract: Many genes are identified as being metastasis associated. Identifying and profiling of these genes expression can be used to evaluate a sample, to diagnose tumor invasive potential or metastatic development in a sample, or screen for a test compound useful in the prevention or treatment of tumor metastasis.
    Type: Application
    Filed: June 25, 2002
    Publication date: March 20, 2003
    Inventors: Jeremy J.W. Chen, Pan-Chyr Yang, Konan Peck, Tse-Ming Hong, Shuenn-Chen Yang, Cheng-Wen Wu
  • Publication number: 20020176811
    Abstract: Apparatuses and methods are described for parallel oligonucleotide synthesis of hundreds of different sequences and lengths at a time. Standard phosphoramidite chemistry is employed. The syntheses take place in a reaction plate compatible with the industrial standard microplate format to allow the use of readily available automated instruments for subsequent processing. Key parameters in reducing synthesis volume in small reaction wells are discussed. This invention provides solutions to the difficulties of low volume, high number synthetic reactions.
    Type: Application
    Filed: August 6, 2001
    Publication date: November 28, 2002
    Inventors: Konan Peck, Ji-Yen Cheng
  • Patent number: 6428667
    Abstract: Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
    Type: Grant
    Filed: October 10, 2000
    Date of Patent: August 6, 2002
    Assignee: The Regents of the University of California, Berkeley
    Inventors: Alexander N. Glazer, Richard A. Mathies, Konan Peck
  • Patent number: 6280933
    Abstract: Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
    Type: Grant
    Filed: November 7, 1997
    Date of Patent: August 28, 2001
    Assignee: The Regents of the University of California
    Inventors: Alexander N. Glazer, Richard A. Mathies, Konan Peck
  • Patent number: 6218114
    Abstract: The invention relates to a method of detecting a differentially expressed gene in a first sample of nucleic acids representing a first population of RNA transcripts and a second sample of nucleic acids representing a second population of RNA transcripts. The nucleic acids in the samples are labled with a member of specific binding pair, and the labeled nucleic acids in each sample are then hybridized to an excess of copies of a gene-specific sequence. The hybridized nucleic acids in each sample are further labeled by binding a second member of the specific binding pair to the first member, in which the second member has an activity to convert a chromogenic substrate into a chromogen. As a result of contacting the second member with the chromogenic substrate, the chromogenic substrate is converted into the chromogen. A difference in the amounts of chromogen produced from assaying the two samples indicate that the gene-specific sequence is differentially expressed in the samples.
    Type: Grant
    Filed: March 27, 1998
    Date of Patent: April 17, 2001
    Assignee: Academia Sinica
    Inventors: Konan Peck, Jeremy J. W. Chen, Pan-Chyr Yang, Reen Wu, Fu Chang, Yi-Wen Chu, Cheng-Wen Wu
  • Patent number: 5789173
    Abstract: The present invention discloses a method for rapid antimicrobial susceptibility testing to screen antibiotics in a few hours instead of days by conventional methods. This method can also be used to identify susceptible antibiotics to treat mycobacterial infection in a few days instead of the usual six to eight weeks. Fast screening of antibiotics is achieved by a short period of specimen incubation in different antibiotics embedded media to create differential bacterial counts. The differences of bacterial counts among antibiotics embedded media are subsequently amplified by DNA amplification methods for detection. Following DNA amplification, rapid quantitation and minimum inhibition concentration (MIC) determinations for a panel of antibiotics are achieved in less than one minute by fluorescence quantitation methods.
    Type: Grant
    Filed: August 2, 1996
    Date of Patent: August 4, 1998
    Assignee: Academia Sinica
    Inventors: Konan Peck, Pan-Chyr Yang, Shu-Li Wung
  • Patent number: 5763162
    Abstract: Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
    Type: Grant
    Filed: December 2, 1993
    Date of Patent: June 9, 1998
    Assignee: The Regents of University of California
    Inventors: Alexander N. Glazer, Richard A. Mathies, Konan Peck
  • Patent number: 5091652
    Abstract: A fluorescent scanner for scanning the fluorescence from a fluorescence labeled separated sample on a sample carrier including a confocal microscope for illuminating a predetermined volume of the sample carrier and/or receiving and processing fluorescence emissions from said volume to provide a display of the separated sample.
    Type: Grant
    Filed: June 1, 1990
    Date of Patent: February 25, 1992
    Assignee: The Regents of the University of California
    Inventors: Richard A. Mathies, Konan Peck