Patents by Inventor Konstantin Konstantinov
Konstantin Konstantinov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11912739Abstract: Provided herein are methods of performing chromatography with gamma-irradiated chromatography resin that include providing a chromatography column including a gamma-irradiated chromatography resin; performing a first cycle of chromatography through the column, where the cycle includes exposing the chromatography resin to a denaturing buffer; and performing at least one additional cycle of chromatography through the column. Also provided are integrated, closed or substantially closed, and continuous processes for manufacturing of a recombinant protein that include the use of at least one chromatography column including gamma-irradiated chromatography resin, where the gamma-irradiated chromatography resin is exposed to denaturing buffer during each cycle in the process, and reduced bioburden buffer is used in the process.Type: GrantFiled: August 14, 2020Date of Patent: February 27, 2024Assignee: Genzyme CorporationInventors: Rahul Godawat, Veena Warikoo, Rohan Patil, Konstantin Konstantinov, Venkat Kishore Ryakala, Mahsa Rohani
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Patent number: 11884703Abstract: Provided herein are methods of processing a fluid that include the use of one or both of a circuit system including a tangential flow filtration (TFF) unit and a circuit system including a tangential flow virus filtration (TFVF) unit. Also provided are integrated and continuous processes for manufacturing a therapeutic protein drug substance that include a step of processing a fluid including the recombinant therapeutic protein using any of the methods provided herein.Type: GrantFiled: February 19, 2021Date of Patent: January 30, 2024Assignee: Genzyme CorporationInventors: Tarl Vetter, Michael Coolbaugh, Veena Warikoo, Konstantin Konstantinov
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Publication number: 20240002785Abstract: Described herein are methods for the production of extracellular vesicles comprising culturing a population of producer cells in single-cell suspension, wherein the cells are cultured in chemically-defined culture medium, wherein the culture medium lacks animal-derived serum and animal-derived components; and obtaining from the cell culture an extracellular vesicle preparation comprising extracellular vesicles. In certain embodiments, the methods comprise perfusion culturing methods, including single-cell perfusion culturing methods and batch-refeed culturing methods. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.Type: ApplicationFiled: February 21, 2023Publication date: January 4, 2024Applicant: LONZA SALES AGInventors: Agata A. VILLIGER, Andrew F. GRUBE, Tik Yan CHAN, Scott D. ESTES, Kathryn E. GOLDEN, Konstantin KONSTANTINOV, Douglas E. WILLIAMS
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Patent number: 11839861Abstract: Provided herein are methods of reducing bioburden of (e.g., sterilizing) a chromatography resin that include exposing a container including a composition including a chromatography resin and at least one antioxidant agent and/or chelator to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chromatography resin, where the at least one antioxidant agent and/or chelator are present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after/upon exposure to the dose of gamma-irradiation. Also provided are reduced bioburden chromatography columns including the reduced bioburden chromatography resin, compositions including a chromatography resin and at least one chelator and/or antioxidant agent, methods of performing reduced bioburden column chromatography using one of these reduced bioburden chromatography columns, and integrated, closed, and continuous processes for reduced bioburden manufacturing of a purified recombinant protein.Type: GrantFiled: January 14, 2021Date of Patent: December 12, 2023Assignee: Genzyme CorporationInventors: Rahul Godawat, Veena Warikoo, Rohan Patil, Konstantin Konstantinov, Venkat Kishore Ryakala
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Publication number: 20230301917Abstract: Methods of loading extracellular vesicles with payload molecules via homogenization are disclosed herein.Type: ApplicationFiled: October 11, 2022Publication date: September 28, 2023Applicant: Codiak BioSciences, Inc.Inventors: Aaron R. NOYES, Michael P. MERCALDI, Kathryn E. GOLDEN, Raymond W. BOURDEAU, Michael F. DOHERTY, Konstantin KONSTANTINOV, Douglas E. WILLIAMS
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Publication number: 20210268477Abstract: Provided herein are methods of reducing bioburden of (e.g., sterilizing) a chromatography resin that include exposing a container including a composition including a chromatography resin and at least one antioxidant agent and/or chelator to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chromatography resin, where the at least one antioxidant agent and/or chelator are present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after/upon exposure to the dose of gamma-irradiation. Also provided are reduced bioburden chromatography columns including the reduced bioburden chromatography resin, compositions including a chromatography resin and at least one chelator and/or antioxidant agent, methods of performing reduced bioburden column chromatography using one of these reduced bioburden chromatography columns, and integrated, closed, and continuous processes for reduced bioburden manufacturing of a purified recombinant protein.Type: ApplicationFiled: January 14, 2021Publication date: September 2, 2021Applicant: Genzyme CorporationInventors: Rahul Godawat, Veena Warikoo, Rohan Patil, Konstantin Konstantinov, Venkat Kishore Ryakala
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Publication number: 20210269477Abstract: Provided herein are methods of processing a cell culture and open circuit filtration systems.Type: ApplicationFiled: May 14, 2021Publication date: September 2, 2021Applicant: Genzyme CorporationInventors: Konstantin Konstantinov, Benjamin Wright, Jin Yin, Marcella Yu, Hang Zhou
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Publication number: 20210171572Abstract: Provided herein are methods of processing a fluid that include the use of one or both of a circuit system including a tangential flow filtration (TFF) unit and a circuit system including a tangential flow virus filtration (TFVF) unit. Also provided are integrated and continuous processes for manufacturing a therapeutic protein drug substance that include a step of processing a fluid including the recombinant therapeutic protein using any of the methods provided herein.Type: ApplicationFiled: February 19, 2021Publication date: June 10, 2021Applicant: Genzyme CorporationInventors: Tarl Vetter, Michael Coolbaugh, Veena Warikoo, Konstantin Konstantinov
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Patent number: 10968252Abstract: Provided herein are methods of processing a fluid that include the use of one or both of a circuit system including a tangential flow filtration (TFF) unit and a circuit system including a tangential flow virus filtration (TFVF) unit. Also provided are integrated and continuous processes for manufacturing a therapeutic protein drug substance that include a step of processing a fluid including the recombinant therapeutic protein using any of the methods provided herein.Type: GrantFiled: August 15, 2017Date of Patent: April 6, 2021Assignee: Genzyme CorporationInventors: Tarl Vetter, Michael Coolbaugh, Veena Warikoo, Konstantin Konstantinov
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Publication number: 20210093568Abstract: Methods of loading extracellular vesicles with payload molecules via homogenization are disclosed herein.Type: ApplicationFiled: September 8, 2020Publication date: April 1, 2021Inventors: Aaron R. NOYES, Michael P. MERCALDI, Kathryn E. GOLDEN, Raymond W. BOURDEAU, Michael F. DOHERTY, Konstantin KONSTANTINOV, Douglas E. WILLIAMS
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Patent number: 10919021Abstract: Provided herein are methods of reducing bioburden of (e.g., sterilizing) a chromatography resin that include exposing a container including a composition including a chromatography resin and at least one antioxidant agent and/or chelator to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chromatography resin, where the at least one antioxidant agent and/or chelator are present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after/upon exposure to the dose of gamma-irradiation. Also provided are reduced bioburden chromatography columns including the reduced bioburden chromatography resin, compositions including a chromatography resin and at least one chelator and/or antioxidant agent, methods of performing reduced bioburden column chromatography using one of these reduced bioburden chromatography columns, and integrated, closed, and continuous processes for reduced bioburden manufacturing of a purified recombinant protein.Type: GrantFiled: August 21, 2018Date of Patent: February 16, 2021Assignee: Genzyme CorporationInventors: Rahul Godawat, Veena Warikoo, Rohan Patil, Konstantin Konstantinov, Venkat Kishore Ryakala
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Publication number: 20200377850Abstract: Provided herein are seed train processes and methods of producing a recombinant protein that include the use of these seed train processes.Type: ApplicationFiled: February 24, 2020Publication date: December 3, 2020Applicant: Genzyme CorporationInventors: Michael Bruninghaus, Konstantin Konstantinov, Benjamin Wright, Weichang Zhou
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Publication number: 20200369718Abstract: Provided herein are methods of performing chromatography with gamma-irradiated chromatography resin that include providing a chromatography column including a gamma-irradiated chromatography resin; performing a first cycle of chromatography through the column, where the cycle includes exposing the chromatography resin to a denaturing buffer; and performing at least one additional cycle of chromatography through the column. Also provided are integrated, closed or substantially closed, and continuous processes for manufacturing of a recombinant protein that include the use of at least one chromatography column including gamma-irradiated chromatography resin, where the gamma-irradiated chromatography resin is exposed to denaturing buffer during each cycle in the process, and reduced bioburden buffer is used in the process.Type: ApplicationFiled: August 14, 2020Publication date: November 26, 2020Applicant: Genzyme CorporationInventors: Rahul Godawat, Veena Warikoo, Rohan Patil, Konstantin Konstantinov, Venkat Kishore Ryakala, Mahsa Rohani
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Publication number: 20200317726Abstract: Provided herein are integrated continuous biomanufacturing processes for producing a therapeutic protein drug substance. Also provided are systems that are capable of continuously producing a therapeutic protein drug substance.Type: ApplicationFiled: June 22, 2020Publication date: October 8, 2020Applicant: Genzyme CorporationInventors: Konstantin Konstantinov, Rahul Godawat, Veena Warikoo, Sujit Jain
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Patent number: 10711034Abstract: Provided herein are integrated continuous biomanufacturing processes for producing a therapeutic protein drug substance. Also provided are systems that are capable of continuously producing a therapeutic protein drug substance.Type: GrantFiled: April 21, 2017Date of Patent: July 14, 2020Assignee: Genzyme CorporationInventors: Konstantin Konstantinov, Rahul Godawat, Veena Warikoo, Sujit Jain
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Patent number: 10570367Abstract: Provided herein are seed train processes and methods of producing a recombinant protein that include the use of these seed train processes.Type: GrantFiled: June 8, 2015Date of Patent: February 25, 2020Assignee: Genzyme CorporationInventors: Michael Bruninghaus, Konstantin Konstantinov, Benjamin Wright, Weichang Zhou
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Publication number: 20190175506Abstract: Methods of loading extracellular vesicles with payload molecules via homogenization are disclosed herein.Type: ApplicationFiled: November 13, 2018Publication date: June 13, 2019Inventors: Aaron R. Noyes, Michael P. Mercaldi, Kathryn E. Golden, Raymond W. Bourdeau, Michael F. Doherty, Konstantin Konstantinov, Douglas E. Williams
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Publication number: 20190085284Abstract: Described herein are methods for the production of extracellular vesicles comprising culturing a population of producer cells in single-cell suspension, wherein the cells are cultured in chemically-defined culture medium, wherein the culture medium lacks animal-derived serum and animal-derived components; and obtaining from the cell culture an extracellular vesicle preparation comprising extracellular vesicles. In certain embodiments, the methods comprise perfusion culturing methods, including single-cell perfusion culturing methods and batch-refeed culturing methods. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.Type: ApplicationFiled: September 20, 2018Publication date: March 21, 2019Inventors: Agata A. Villiger, Andrew F. Grube, Tik Yan Chan, Scott D. Estes, Kathryn E. Golden, Konstantin Konstantinov, Douglas E. Williams
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Publication number: 20180369786Abstract: Provided herein are methods of reducing bioburden of (e.g., sterilizing) a chromatography resin that include exposing a container including a composition including a chromatography resin and at least one antioxidant agent and/or chelator to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chromatography resin, where the at least one antioxidant agent and/or chelator are present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after/upon exposure to the dose of gamma-irradiation. Also provided are reduced bioburden chromatography columns including the reduced bioburden chromatography resin, compositions including a chromatography resin and at least one chelator and/or antioxidant agent, methods of performing reduced bioburden column chromatography using one of these reduced bioburden chromatography columns, and integrated, closed, and continuous processes for reduced bioburden manufacturing of a purified recombinant protein.Type: ApplicationFiled: August 21, 2018Publication date: December 27, 2018Applicant: Genzyme CorporationInventors: Rahul Godawat, Veena Warikoo, Rohan Patil, Konstantin Konstantinov, Venkat Kishore Ryakala
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Publication number: 20180354986Abstract: Provided herein are methods of performing chromatography with gamma-irradiated chromatography resin that include providing a chromatography column including a gamma-irradiated chromatography resin; performing a first cycle of chromatography through the column, where the cycle includes exposing the chromatography resin to a denaturing buffer; and performing at least one additional cycle of chromatography through the column. Also provided are integrated, closed or substantially closed, and continuous processes for manufacturing of a recombinant protein that include the use of at least one chromatography column including gamma-irradiated chromatography resin, where the gamma-irradiated chromatography resin is exposed to denaturing buffer during each cycle in the process, and reduced bioburden buffer is used in the process.Type: ApplicationFiled: August 21, 2018Publication date: December 13, 2018Applicant: Genzyme CorporationInventors: Rahul Godawat, Veena Warikoo, Rohan Patil, Konstantin Konstantinov, Venkat Kishore Ryakala, Mahsa Rohani