Patents by Inventor Konstantine Severinov

Konstantine Severinov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods of ligating expressed proteins
    Patent number: 6875594
    Abstract: The present invention provides a method of cleaving a recombinantly expressed protein from an intein and ligating the protein to a peptide containing an N-terminal cysteine having an unoxidized sulfhydryl side chain which comprises contacting the protein with the peptide in a reaction solution comprising a conjugated thiophenol, thereby forming a C-terminal thioester of the recombinant protein which spontaneously rearranges intramolecularly to form an amide bond linking the protein to the peptide.
    Type: Grant
    Filed: July 12, 2001
    Date of Patent: April 5, 2005
    Assignee: The Rockefeller University
    Inventors: Tom W. Muir, Philip A. Cole, Dolan Sondhi, Konstantine Severinov
  • Methods of ligating expressed proteins
    Publication number: 20020151006
    Abstract: The present invention provides a method of cleaving a recombinantly expressed protein from an intein and ligating the protein to a peptide containing an N-terminal cysteine having an unoxidized sulfhydryl side chain which comprises contacting the protein with the peptide in a reaction solution comprising a conjugated thiophenol, thereby forming a C-terminal thioester of the recombinant protein which spontaneously rearranges intramolecularly to form an amide bond linking the protein to the peptide.
    Type: Application
    Filed: July 12, 2001
    Publication date: October 17, 2002
    Inventors: Tom W. Muir, Philip A. Cole, Jeffrey M. Friedman, Dolan Sondhi, Konstantine Severinov