Abstract: An apparatus for transporting magnetic particles. The apparatus can comprise a substrate that can be configured for rotating around an axis of rotation. Further, fluidic structures can be arranged in the substrate, which can comprise an oblique chamber wall that can be arranged at an angle ? with respect to a plane perpendicular to the axis of rotation. A magnetic force element can be arranged radially inside the oblique chamber wall with respect to the axis of rotation and can be configured to apply a magnetic force to the magnetic particles disposed in the fluidic structures depending on a positional relationship between the magnetic force element and the fluidic structures, wherein the oblique chamber wall is inclined towards the magnetic force element.

Abstract: Disclosed are methods for detecting a target molecule in a sample, including the sequential steps of: (a) incubating at the same time in a buffer solution in a reaction well (i) the sample suspected to contain one or more target molecules, (ii) solid phase-coupled capture molecules suitable for binding to the target molecules, wherein the capture molecules are coupled to beads (capture-beads), (iii) a defined amount of detection molecules suitable for binding to the target molecules and/or the capture molecules, wherein the detection molecules are coupled to beads (detection-beads), wherein the capture-beads are separable from the detection-beads, (b) separating in the reaction well the capture-beads from the buffer solution including unbound detection-beads, and (c) detecting the remaining unbound detection-beads in the buffer solution, which has been separated from the capture-beads, wherein the method does not include a washing step.