Abstract: The present invention relates to a method for producing Cypridina luciferase labeled with hydrophilic biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted with Cypridina luciferase, and biotin-labeled Cypridina luciferase wherein a sugar chain in Cypridina luciferase has been biotinylated.

Abstract: An object of the present invention is to provide a mutant luciferase having luciferase activity with an altered emission spectrum. A specific amino acid residue(s) is substituted in a luciferase derived from Cypridina noctiluca and then the resulting mutant luciferase having luciferase activity with an emission spectrum differing from that of the wild-type luciferase is screened for.

Abstract: The present invention relates to a method for producing Cypridina luciferase labeled with hydrophilic biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted with Cypridina luciferase, and biotin-labeled Cypridina luciferase wherein a sugar chain in Cypridina luciferase has been biotinylated.

Abstract: An object of the present invention is to provide a mutant luciferase having luciferase activity with an altered emission spectrum. A specific amino acid residue(s) is substituted in a luciferase derived from Cypridina noctiluca and then the resulting mutant luciferase having luciferase activity with an emission spectrum differing from that of the wild-type luciferase is screened for.

Abstract: In the evaporative humidifier of the present invention, a desired humidity is obtained by supplying water only to a humidifying module required for humidification. Air simply passes through a humidifying module to which water is not supplied. Accordingly, air obtained by mixing humidified air passing through the humidifying module which is wet with water being supplied thereto (wet zone) and non-humidified air passing through the humidifying module to which water is not supplied (dry zone) is blown out from an outlet of the evaporative humidifier. The mixture amount (mixture ratio) is controlled by the opening and closing of the valve, so that a desired humidity is obtained. Therefore, with the evaporative humidifier of the present invention, humidity control can be accurately performed without unnecessarily operating the humidifying device and without wasting energy.

Abstract: The present invention provides a DNA fragment having a cold-inducible promoter function of yeast, which has high activity in a low temperature range, by identifying a DNA fragment, which exists in a non-translation region located upstream of the 5?-terminal side of a gene selected from the group consisting of cold-inducible genes of Saccharomyces cerevisiae, and has a cold-inducible promoter function.

Abstract: The present invention relates to a method for producing Cypridina luciferase labeled with hydrophilic biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted with Cypridina luciferase, and biotin-labeled Cypridina luciferase wherein a sugar chain in Cypridina luciferase has been biotinylated.

Abstract: The present invention provides a DNA fragment having a cold-inducible promoter function of yeast, which has high activity in a low temperature range, by identifying a DNA fragment, which exists in a non-translation region located upstream of the 5?-terminal side of a gene selected from the group consisting of cold-inducible genes of Saccharomyces cerevisiae, and has a cold-inducible promoter function.