Abstract: The present specification discloses natural repellent compounds, and isomeric forms, structural analogs and derivatives of the said compounds, having repellent activity against mosquitoes and other blood-sucking arthropods, compositions comprising such repellent compounds, methods of screening for such repellent compounds, methods of reducing mosquito bites on an individual and methods of reducing mosquito infestation to a location.

Abstract: The present specification discloses natural repellent compounds, and isomeric forms, structural analogues and derivatives of the said compounds, having repellent activity against mosquitoes and other blood-sucking arthropods, compositions comprising such repellent compounds, methods of screening for such repellent compounds, methods of reducing mosquito bites on an individual and methods of reducing mosquito infestation to a location.

Abstract: The present specification discloses natural repellent compounds, and isomeric forms, structural analogs and derivatives of the said compounds, having repellent activity against mosquitoes and other blood-sucking arthropods, compositions comprising such repellent compounds, methods of screening for such repellent compounds, methods of reducing mosquito bites on an individual and methods of reducing mosquito infestation to a location.

Abstract: The present specification discloses natural repellent compounds, and isomeric forms, structural analogues and derivatives of the said compounds, having repellent activity against mosquitoes and other blood-sucking arthropods, compositions comprising such repellent compounds, methods of screening for such repellent compounds, methods of reducing mosquito bites on an individual and methods of reducing mosquito infestation to a location.

Abstract: The present invention relates to the use of nucleic acids which code for secretory fusion proteins to elicit immune responses. The secretory fusion protein comprises a signal sequence from a secretion competent protein, particularly a cytokine, which is linked in frame with the coding sequence of the antigen of interest which is normally intracellular. The nucleic acid encoding such a fusion protein is significantly more effective in its ability to elicit antibody production than the nucleic acid encoding the intracellular antigen. The present invention thus provides for a method of generating antibodies as well as a method of eliciting protective immune response using such nucleic acids. Also provided are pharmaceutical compositions comprising these nucleic acids.

Abstract: An expression cassette is disclosed which is useful for the secretion of a heterologous protein from mammalian and insect cells. The expression cassette comprises a polynucleotide sequence encoding a secretion competent polypeptide which is linked in frame to a heterologous gene sequence. Also disclosed is a method of secreting heterologous proteins in mammalian and insect cells using the expression cassette.

Abstract: Described herein is a method of expressing heterologous proteins in insect cells using an expression cassette comprising a structural gene for a heterologous protein physically attached to an insect cellular promoter and an enhancer. The cells may also express the IE-1 product. Also described herein is a method of killing insects comprising infecting the insects with a recombinant baculovirus comprising a structural gene for an incompatible protein functionally linked to an insect cellular promoter and an enhancer. The invention is also directed towards expression cassettes comprising an insect cellular promoter functionally linked to an enhancer wherein the promoter is capable of directing the expression of a heterologous protein in tissues containing the expression cassette, recombinant expression cassettes containing heterologous proteins, transplacement fragments, vectors and recombinant baculoviruses.

Abstract: An baculovirus artificial chromosome is disclosed which has the lef-8 gene inactivated. The baculovirus artificial chromosome allows the cloning and expression of heterologous genes in insect and mammalian cells without killing the host cells. Also disclosed is an infectious baculovirus comprising the artificial chromosome and a cell containing the chromosome. Also disclosed is an insect cell which expresses the LEF-8 gene in the absence of a baculovirus chromosome. Also disclosed is a method to express heterologous proteins in eukaryotic cells using the baculovirus artificial chromosome.

Abstract: An expression cassette useful for the secretion of a heterologous protein from eukaryotic cells comprising a DNA sequence encoding a promoter functionally linked to a DNA sequence coding for the juvenile hormone esterase gene which is linked in frame to a heterologous gene is disclosed. Also disclosed is a method of secreting heterologous proteins in eukaryotic cells.

Abstract: Described herein is a method of expressing heterologous proteins in insect cells using an expression cassette comprising a structural gene for a heterologous protein physically attached to an insect cellular promoter and an enhancer. The cells may also express the IE-1 product. Also described herein is a method of killing insects comprising infecting the insects with a recombinant baculovirus comprising a structural gene for an incompatible protein functionally linked to an insect cellular promoter and an enhancer. The invention is also directed towards expression cassettes comprising an insect cellular promoter functionally linked to an enhancer wherein the promoter is capable of directing the expression of a heterologous protein in tissues containing the expression cassette, recombinant expression cassettes containing heterologous proteins, transplacement fragments, vectors and recombinant baculoviruses.

Abstract: Described herein is a method of expressing heterologous proteins in insect cells using an expression cassette comprising a structural gene for a heterologous protein physically attached to an insect cellular promoter and an enhancer. The cells may also express the IE-1 product. Also described herein is a method of killing insects comprising infecting the insects with a recombinant baculovirus comprising a structural gene for an incompatible protein functionally linked to an insect cellular promoter and an enhancer. The invention is also directed towards expression cassettes comprising an insect cellular promoter functionally linked to an enhancer wherein the promoter is capable of directing the expression of a heterologous protein in tissues containing the expression cassette, recombinant expression cassettes containing heterologous proteins, transplacement fragments, vectors and recombinant baculoviruses.