Abstract: [Problem] To evaluate objective cell groups by estimating a mixing ratio of objective cell group without affecting treatment itself and its production process when the objective cell groups include plural kinds of cell groups having different attributes, and to provide an image analysis system and a culture management system capable of accurately perform a quality control and a production control with low cost. [Solution] The cell quality evaluation system 1 detects a feature amount in each cell easily analyzable from images, and estimates a mixing ratio of each of plural kinds of cell groups included in the objective cell groups based on a distribution of the detected feature amount and pre-recorded information of the feature amount. As the feature amount, the embodiment uses a migration speed of each cell, easily distinguishable by analyzing tracking of each cell from plural images in time series.

Abstract: A liquid transport method is disclosed for efficiently producing cell cultures. The liquid transport method of the present disclosure, which is in processing of cells, includes: a) a step in which a container holding a liquid is gripped by a gripping tool of a robot; and b) a step in which the liquid in the container is transported to a collection container by rotating the gripped container. In steps a) and b) the robot operates such as not to pass over a vertical line of an opening of the collection container.

Abstract: A method for evaluating the ability of cells to grow into a sheet includes (1) a step of examining cell cultures growing into a sheet to determine their impedance and/or electrical resistance or numerical values relating thereto; (2) a step of comparing the numerical values determined by Step (1) with reference values; and (3) a step of judging, based on the results of comparison obtained in Step (2), the ability of cells to grow into a sheet.

Abstract: Disclosed are a cell processing method, a device, and a robot system that has a simple constitution and are reasonable. A robot system is provided for injecting liquid by rotating an injection container containing the liquid and an injection volume of the liquid is constant around an axis vertical to a long axis of the injection container. The robot system executes an injection start control to rotate the injection container around a predetermined axis, an injection control to stop rotation for a predetermined time and inject the liquid, and an injection end control to reversely rotate the injection container around the predetermined axis, and the predetermined time is calculated based on an injection flow rate measured in real time.

Abstract: A method is disclosed for recovering living cells highly efficiently from cryopreserved cells by thawing and a system designed for such a method. The method for recovering living cells from cryopreserved cells includes thawing cryopreserved cells and diluting the thawed cell suspension with a diluent, wherein the dilution is performed in such a way that the maximum load of osmotic pressure at the time of dilution is equal to or less than 250 mOsm/second.

Abstract: A liquid transport method is disclosed for efficiently producing cell cultures. The liquid transport method of the present disclosure, which is in processing of cells, includes: a) a step in which a container holding a liquid is gripped by a gripping tool of a robot; and b) a step in which the liquid in the container is transported to a collection container by rotating the gripped container. In steps a) and b) the robot operates such as not to pass over a vertical line of an opening of the collection container.

Abstract: A liquid transport method is disclosed for efficiently producing cell cultures. The liquid transport method of the present disclosure, which is in processing of cells, includes: a) a step in which a container holding a liquid is gripped by a gripping tool of a robot; and b) a step in which the liquid in the container is transported to a collection container by rotating the gripped container. In steps a) and b) the robot operates such as not to pass over a vertical line of an opening of the collection container.

Abstract: A sheet-shaped cell culture and method for producing a sheet-shaped cell culture are disclosed having a high activity such as high cytokine productivity and a production method thereof. The method for producing a sheet-shaped cell culture can include freezing cells, thawing the frozen cells and forming a sheet-shaped cell culture. A sheet-shaped cell culture which is produced by the production method and which has a high activity and a method for treating a disease associated with an abnormality of a tissue with the sheet-shaped cell culture.

Abstract: [Problem] To evaluate objective cell groups by estimating a mixing ratio of objective cell group without affecting treatment itself and its production process when the objective cell groups include plural kinds of cell groups having different attributes, and to provide an image analysis system and a culture management system capable of accurately perform a quality control and a production control with low cost. [Solution] The cell quality evaluation system 1 detects a feature amount in each cell easily analyzable from images, and estimates a mixing ratio of each of plural kinds of cell groups included in the objective cell groups based on a distribution of the detected feature amount and pre-recorded information of the feature amount. As the feature amount, the embodiment uses a migration speed of each cell, easily distinguishable by analyzing tracking of each cell from plural images in time series.

Abstract: A tissue collection device including a sheath member that has a first threaded portion on the inner surface of the sheath member and a puncture tube positionable in the interior of the sheath member. The puncture tube is axially movable in distal and proximal directions relative to the sheath member. The puncture tube has a second threaded portion on the outer surface of the puncture tube that threadedly engages with the first threaded portion when the puncture tube is within the interior of the sheath member. At least one of the distal end of the sheath member and the distal end of the puncture tube includes a blade to incise tissue from a living body.

Abstract: A method, a device, and an annular member are disclosed for keeping a shape of a fragile material in a liquid in a container. The method includes covering a surface of the liquid with a sealing material configured to melt and solidify according to a temperature change; and solidifying the sealing material to cover the surface of the liquid. The device includes a container configured to hold the fragile material; and a sealing material configured to melt and solidify according to a temperature change. The annular member includes an upper annular groove configured to receive a sealing material, the sealing material configured to melt and solidify according to a temperature change, the upper annular groove having an inner wall and an outer wall, and wherein a height of the inner wall is lower than a height of the outer wall.

Abstract: A method for evaluating the ability of cells to grow into a sheet includes (1) a step of examining cell cultures growing into a sheet to determine their impedance and/or electrical resistance or numerical values relating thereto; (2) a step of comparing the numerical values determined by Step (1) with reference values; and (3) a step of judging, based on the results of comparison obtained in Step (2), the ability of cells to grow into a sheet.

Abstract: A cell culture transferring instrument includes a cylindrical body held by an operator not shown in the drawing, a plunger inserted through the interior of the cylindrical body, a plurality of support members connected to a distal end of the plunger, and an adsorbent capable of adsorbing a sheet-shaped cell culture held by the support members. The support members in contact with the distal end of the cylindrical body are elastically deformed by displacing the plunger toward the proximal end side with respect to the cylindrical body in a state in which the sheet-shaped cell culture is adsorbed to the adsorbent, whereby the adsorbent and the sheet-shaped cell culture are deformed and housed in a housing hole in the cylindrical body.

Abstract: A liquid transport method is disclosed for efficiently producing cell cultures. The liquid transport method of the present disclosure, which is in processing of cells, includes: a) a step in which a container holding a liquid is gripped by a gripping tool of a robot; and b) a step in which the liquid in the container is transported to a collection container by rotating the gripped container. In steps a) and b) the robot operates such as not to pass over a vertical line of an opening of the collection container.

Abstract: A medical device is disclosed, which is capable of treating cardiac insufficiency, on a causal therapy basis. The medical device is a heart harness composed of a porous hollow structure. A method of treating a heart disease includes applying the medical device as above to a heart of a subject in need thereof.

Abstract: A method is disclosed for recovering living cells highly efficiently from cryopreserved cells by thawing and a system designed for such a method. The method for recovering living cells from cryopreserved cells includes thawing cryopreserved cells and diluting the thawed cell suspension with a diluent, wherein the dilution is performed in such a way that the maximum load of osmotic pressure at the time of dilution is equal to or less than 250 mOsm/second.

Abstract: A sheet-shaped cell culture and method for producing a sheet-shaped cell culture are disclosed having a high activity such as high cytokine productivity and a production method thereof. The method for producing a sheet-shaped cell culture can include freezing cells, thawing the frozen cells and forming a sheet-shaped cell culture. A sheet-shaped cell culture which is produced by the production method and which has a high activity and a method for treating a disease associated with an abnormality of a tissue with the sheet-shaped cell culture.

Abstract: A cell culture transferring instrument includes a cylindrical body held by an operator not shown in the drawing, a plunger inserted through the interior of the cylindrical body, a plurality of support members connected to a distal end of the plunger, and an adsorbent capable of adsorbing a sheet-shaped cell culture held by the support members. The support members in contact with the distal end of the cylindrical body are elastically deformed by displacing the plunger toward the proximal end side with respect to the cylindrical body in a state in which the sheet-shaped cell culture is adsorbed to the adsorbent, whereby the adsorbent and the sheet-shaped cell culture are deformed and housed in a housing hole in the cylindrical body.