Patents by Inventor Ko-Woon Lee

Ko-Woon Lee has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for generating recombinant DNA library using unidirectional single-stranded DNA fragments
    Patent number: 6955879
    Abstract: The present invention relates to a method for producing a recombinant polynucleotides comprising the steps of generating a pool of unidirectional single-stranded polynucleotide fragments from two or more homologous double-stranded polynucleotides, conducting a polymerization process comprising multi-cyclic extension reactions using the unidirectional single-stranded polynucleotide fragments as templates and specific oligonucleotides as primers to obtain recombinant polynucleotides, and conducting a polymerase chain reaction using at least one primer to amplify the recombinant polynucleotides; and a method for constructing a recombinant DNA library comprising the steps of inserting the recombinant polynucleotide prepared by the above method into a vector and transforming an expression cell with said vector containing the recombinant polynucleotide to obtain a plurality of mutant clones.
    Type: Grant
    Filed: June 16, 2001
    Date of Patent: October 18, 2005
    Assignee: Amicogen, Inc.
    Inventors: Si-Hyoung Lee, Yong-Chul Shin, Yeong-Joong Jeon, Kyung-Hwa Jung, Eun-Jung Ryu, Ko-Woon Lee
  • Method for generating recombinant dna library using unidirectional single-stranded dna fragments
    Publication number: 20030152943
    Abstract: The present invention relates to a method for producing a recombinant polynucleotides comprising the steps of generating a pool of unidirectional single-stranded polynucleotide fragments from two or more homologous double-stranded polynucleotides, conducting a polymerization process comprising multi-cyclic extension reactions using the unidirectional single-stranded polynucleotide fragments as templates and specific oligonucleotides as primers to obtain recombinant polynucleotides, and conducting a polymerase chain reaction using at least one primer to amplify the recombinant polynucleotides; and a method for constructing a recombinant DNA library comprising the steps of inserting the recombinant polynucleotide prepared by the above method into a vector and transforming an expression cell with said vector containing the recombinant polynucleotide to obtain a plurality of mutant clones.
    Type: Application
    Filed: May 28, 2002
    Publication date: August 14, 2003
    Inventors: Si-Hyoung Lee, Yong-Chul Shin, Yeong-Joong Jeon, Kyung-Hwa Jung, Eun-Jung Ryu, Ko-Woon Lee