Abstract: An image acquisition device includes a spatial light modulator modulating irradiation light, a control unit controlling a modulating pattern so that first and second light converging points are formed in an observation object, a light converging optical system converging the irradiation light, a scanning unit scanning positions of the first and second light converging points in the observation object in a scanning direction intersecting an optical axis of the light converging optical system, and a photodetector detecting first observation light generated from the first light converging point and second observation light generated from the second light converging point. The photodetector has a first detection area for detecting the first observation light and a second detection area for detecting the second observation light. The positions of the first and second light converging points are different from each other in a direction of the optical axis.

Abstract: An imaging system includes a light source for outputting initial pulsed light, a polarization control unit for rotating a polarization plane of the initial pulsed light, an optical pulse shaping unit for inputting the initial pulsed light with the rotated polarization plane, and outputting first pulsed light Lp1 having a first polarization direction and second pulsed light Lp2 having a second polarization direction different from the first polarization direction with a time, an irradiation optical system for irradiating an imaging object with the pulsed light Lp1 and the pulsed light Lp2, a light separation element for separating the pulsed light Lp1 and the pulsed light Lp2 reflected by or transmitted through the imaging object on the basis of the polarization directions, an imaging unit for imaging the pulsed light Lp1, and an imaging unit for imaging the pulsed light Lp2.

Abstract: An iterative Fourier transform unit of a modulation pattern calculation apparatus performs a Fourier transform on a waveform function including an intensity spectrum function and a phase spectrum function, performs a replacement of a temporal intensity waveform function based on a desired waveform after the Fourier transform, and then performs an inverse Fourier transform. The iterative Fourier transform unit performs the replacement using a result of multiplying a function representing the desired waveform by a coefficient. The coefficient has a value with which a difference between the function after the multiplication of the coefficient and the temporal intensity waveform function after the Fourier transform is smaller than a difference before the multiplication, and a ratio of the difference is smaller when an intensity is higher at each time of the function before the multiplication.

Abstract: A photostimulation apparatus includes an objective lens arranged to face a biological object, a light source configured to output light to be radiated toward the biological object via the objective lens, a shape acquisition unit configured to acquire information about a shape with a refractive index difference in the biological object, a hologram generation unit configured to generate aberration correction hologram data for correcting aberrations due to the shape with the refractive index difference on the basis of the information acquired by the shape acquisition unit, and a spatial light modulator on which a hologram based on the aberration correction hologram data is presented and which modulates the light output from the light source.

Abstract: A data generating device sets an initial candidate solution of an intensity spectrum function, a phase spectrum function, and an initial temperature and a cooling rate, generates a neighborhood solution, transforms a first waveform function of a frequency domain including the neighborhood solution and the phase spectrum function into a second waveform function of a time domain including a time-intensity waveform function and a time-phase waveform function and calculates an evaluation value representing a degree of difference between the time-intensity waveform function and the desired time-intensity waveform, sets the neighborhood solution as an n-th candidate solution for a certain probability, and lowers the temperature on the basis of the cooling rate. A decrease in the temperature acts in a direction in which the probability P is lowered when the evaluation value of the neighborhood solution is worse than the evaluation value of the candidate solution.

Abstract: An intensity spectrum designing unit of a data generating device includes an initial value setting unit that sets a plurality of objects of a first generation of an intensity spectrum function A(?) and a phase spectrum function ?(?), an evaluation value calculating unit that calculates an evaluation value for each of a plurality of objects of an n-th generation, an object selecting unit that selects two or more objects used for generating a plurality of objects of an (n+1)-th generation among objects of the n-th generation on the basis of superiority of the evaluation value, and a next-generation generating unit that generates a plurality of objects of the (n+1)-th generation on the basis of the selected two or more objects. The evaluation value calculating unit, the object selecting unit, and the next-generation generating unit repeat processes while 1 is added to n until a predetermined condition is satisfied.

Abstract: In a waveform measurement method, first, initial pulsed light is spatially dispersed for respective wavelengths. Next, the initial pulsed light is input to a polarization dependent type SLM in a state where a polarization plane is inclined with respect to a modulation axis direction, and a phase spectrum of a first polarization component of the initial pulsed light along the modulation axis direction is modulated, to cause a time difference between first pulsed light Lp1 including the first polarization component and second pulsed light Lp2 including a second polarization component orthogonal to the first polarization component. After combining the wavelength components, an object is irradiated with the pulsed light Lp1 and the pulsed light Lp2, and light generated in the object is detected. The above detection operation is performed while changing the time difference, and a temporal waveform of the pulsed light Lp1 is obtained.

Abstract: A Fourier transform is performed on a first waveform function in a frequency domain, and a second waveform function in a time domain including a temporal intensity waveform function and a temporal phase waveform function is generated. A replacement of the temporal intensity waveform function based on a desired waveform is performed for the second waveform function. The second waveform function is modified so as to bring a spectrogram of the second waveform function close to a target spectrogram generated in advance in accordance with a desired wavelength band. An inverse Fourier transform is performed on the modified second waveform function, and a third waveform function in the frequency domain is generated. Data is generated on the basis of an intensity spectrum function or a phase spectrum function of the third waveform function.

Abstract: A microscope apparatus (1A) includes a biological sample table (11) that supports the biological sample (B), an objective lens (12) disposed to face the biological sample table (11), a laser light source (13) that outputs light with which the biological sample (B) is irradiated via the objective lens (12), a shape measurement unit (20) that acquires a surface shape of the biological sample (B), a control unit (40) that generates aberration correction hologram data for correcting an aberration caused by the surface shape of the biological sample (B) on the basis of information acquired in the shape measurement unit (20), a first spatial light modulator (33) to which a hologram based on the aberration correction hologram data is presented and that modulates the light output from the laser light source (31), and a photodetector (37) that detects an intensity of light to be detected (L2) generated in the biological sample (B).

Abstract: A pulsed light generation apparatus includes a dispersing unit for dispersing pulsed light for respective wavelengths, a polarization dependent type spatial light modulator for modulating the dispersed pulsed light in respective wavelengths, and a combining unit for combining wavelength components of the pulsed light output from the spatial light modulator. A polarization plane of the pulsed light input to the spatial light modulator is inclined with respect to a polarization direction in which the spatial light modulator has a modulation function. The spatial light modulator causes a time difference between a first polarization component of the pulsed light along the polarization direction and a second polarization component of the pulsed light intersecting with the first polarization component.

Abstract: In a waveform measurement method, first, initial pulsed light is spatially dispersed for respective wavelengths. Next, the initial pulsed light is input to a polarization dependent type SLM in a state where a polarization plane is inclined with respect to a modulation axis direction, and a phase spectrum of a first polarization component of the initial pulsed light along the modulation axis direction is modulated, to cause a time difference between first pulsed light Lp1 including the first polarization component and second pulsed light Lp2 including a second polarization component orthogonal to the first polarization component. After combining the wavelength components, an object is irradiated with the pulsed light Lp1 and the pulsed light Lp2, and light generated in the object is detected. The above detection operation is performed while changing the time difference, and a temporal waveform of the pulsed light Lp1 is obtained.

Abstract: An iterative Fourier transform unit in a modulation pattern calculation apparatus performs a Fourier transform on a waveform function including an intensity spectrum function and a phase spectrum function, performs a replacement of a temporal intensity waveform function based on a desired waveform after the Fourier transform, and then performs an inverse Fourier transform. The iterative Fourier transform unit performs the replacement using a result of multiplying a function representing the desired waveform by a coefficient, and the coefficient has a value in which a difference between the function after the multiplication and the temporal intensity waveform function after the Fourier transform is smaller than a difference before the multiplication of the coefficient.

Abstract: A photostimulation apparatus includes an objective lens arranged to face a biological object, a light source configured to output light to be radiated toward the biological object via the objective lens, a shape acquisition unit configured to acquire information about a shape with a refractive index difference in the biological object, a hologram generation unit configured to generate aberration correction hologram data for correcting aberrations due to the shape with the refractive index difference on the basis of the information acquired by the shape acquisition unit, and a spatial light modulator on which a hologram based on the aberration correction hologram data is presented and which modulates the light output from the light source.

Abstract: A modulation pattern calculation apparatus includes an iterative Fourier transform unit, a filtering process unit, and a modulation pattern calculation unit. The iterative Fourier transform unit performs a Fourier transform on a waveform function including an intensity spectrum function and a phase spectrum function, performs a replacement of a temporal intensity waveform function based on a desired waveform after the Fourier transform and then performs an inverse Fourier transform, and performs a replacement to constrain the phase spectrum function after the inverse Fourier transform. The filtering process unit performs a filtering process of cutting a part exceeding a cutoff intensity for each wavelength, on the intensity spectrum function in a frequency domain.

Abstract: An iterative Fourier transform unit in a modulation pattern calculation apparatus performs a Fourier transform on a waveform function including an intensity spectrum function and a phase spectrum function, performs a replacement of a temporal intensity waveform function based on a desired waveform after the Fourier transform, and then performs an inverse Fourier transform. The iterative Fourier transform unit performs the replacement using a result of multiplying a function representing the desired waveform by a coefficient, and the coefficient has a value in which a difference between the function after the multiplication and the temporal intensity waveform function after the Fourier transform is smaller than a difference before the multiplication of the coefficient.

Abstract: A wavelength conversion element includes a crystal having a periodically poled structure in which polarization is inverted with an inversion period ? along a z-axis which is an input axis of a light pulse. The wavelength conversion element is configured to generate an output the inversion period ?(x) at each position x by change of the inversion period ? according to the position x, and when a target frequency linearly changing with the position x is set to fT(x)=b+ax, a frequency width of the output frequency is set to ?f(x), and the output frequency is set to f(x)=fT(x)+?(x), the output frequency is set to coincide with the target frequency within a range satisfying a condition |?(x)|??f(x).

Abstract: A photostimulation apparatus includes an objective lens arranged to face a biological object, a light source configured to output light to be radiated toward the biological object via the objective lens, a shape acquisition unit configured to acquire information about a shape with a refractive index difference in the biological object, a hologram generation unit configured to generate aberration correction hologram data for correcting aberrations due to the shape with the refractive index difference on the basis of the information acquired by the shape acquisition unit, and a spatial light modulator on which a hologram based on the aberration correction hologram data is presented and which modulates the light output from the light source.

Abstract: A waveform measurement device includes an input spectrum acquisition unit for acquiring an input intensity spectrum being an intensity spectrum of pulsed light, an optical element inputting the pulsed light and outputting light having an intensity spectrum corresponding to a phase spectrum of the pulsed light, an output spectrum acquisition unit for acquiring an output intensity spectrum being an intensity spectrum of the light output from the optical element, and a phase spectrum determination unit for determining the phase spectrum of the pulsed light by comparing an output intensity spectrum calculated when the pulsed light having an input intensity spectrum and a virtual phase spectrum is assumed to be input to the optical element with the output intensity spectrum acquired in the output spectrum acquisition unit. The phase spectrum determination unit sets the virtual phase spectrum by deforming the control phase spectrum.

Abstract: A control apparatus includes a lens, an SLM presenting a modulation pattern on a modulation plane and outputting modulated light L2 for forming light spots P1 and P2 on a pupil plane of the lens, an imaging device imaging a fringe pattern image formed on a focal plane of the lens and generating image data Da indicating the fringe pattern image, a calculation unit calculating at least one kind of parameter among an intensity amplitude, a phase shift amount, and an intensity average from the image data Da, an analysis unit obtaining a deviation in relative positions of an optical axis of the lens and a reference coordinate of the modulation plane based on the parameter, and a changing unit changing an origin position of the reference coordinate so that the deviation in the relative positions is decreased.

Abstract: A microscope apparatus (1A) includes a biological sample table (11) that supports the biological sample (B), an objective lens (12) disposed to face the biological sample table (11), a laser light source (13) that outputs light with which the biological sample (B) is irradiated via the objective lens (12), a shape measurement unit (20) that acquires a surface shape of the biological sample (B), a control unit (40) that generates aberration correction hologram data for correcting an aberration caused by the surface shape of the biological sample (B) on the basis of information acquired in the shape measurement unit (20), a first spatial light modulator (33) to which a hologram based on the aberration correction hologram data is presented and that modulates the light output from the laser light source (31), and a photodetector (37) that detects an intensity of light to be detected (L2) generated in the biological sample (B).