Abstract: The disclosure herein describes a message ordering system for processing and synchronizing chat messages in partitions to maintain messaging order and load balancing in a distributed system at scale. Messages are placed in partitions based on session identifiers (IDs). Messaging order is secured by a session receiver holding a lock over particular partitions. Receivers having subscription to messages in service bus are enabled to terminate on occurrence of a predetermined event, such as threshold wait time without receiving additional messages with a selected session ID from their partitions, activity level on a node, number of receivers on a node or other metrics. Session ID locks are released after a session ends, a receiver terminates or a node crashes for failure handling. New receivers are created to handle new incoming messages for additional load balancing and/or failover.

Abstract: A system and method for providing customized content recommendations to a user based on extrapolated data is described. The system may receive an answer to a question from a user and determine a second unanswered question having a threshold relationship level with the question. The system may compute a predicted probability that the user would answer the second question correctly, calculate a first comparative skill level of the user among a cohort of similar users, and rank the first comparative skill level of the user against a second comparative skill level of the cohort to determine a skill gap of the user. Further, in some implementations, the system may generate a search query based on the skill gap of the user, and determine recommended content customized to the skill gap of the user.

Abstract: The disclosed technology is generally directed to online chat management services. In one example of the technology, organization information is accessed for tenants. The organization information includes, for each organization, configuration information for the organization. Each organization belongs to a corresponding tenant. Worker service instances are scheduled to perform fills associated with the organizations, such that sufficient fills are performed for each organization according to the configuration information for the organizations in the accessed organization information. Each fill includes: causing artifacts associated with a function to be created, such that the artifacts associated with the function are configured based on the corresponding tenant. Artifact information associated with the created artifacts is received, including at least one unique identifier associated with the created artifacts for the function.

Abstract: The invention provides methods of synthesizing a product DNA molecule having a desired and/or defined sequence. The methods involve annealing at least one long oligonucleotide and at least one short oligonucleotide to at least one anchor strand having a sequence at least partially complementary to the at least one long and at least one short oligonucleotide. After annealing, at least one long oligonucleotide bound to an anchor strand abuts at least one short oligonucleotide bound to the same anchor strand. The anchor strand has one or more non-standard nucleotides, and optionally one or more degenerate nucleotides. The method involves ligating the abutting at least one long oligonucleotide and at least one short oligonucleotide to form a dsDNA molecule. The invention also provides methods of synthesizing DNA molecules by assembling oligonucleotide members of a library that contains less than 20,000 members that can be assembled into all possible DNA sequences.

Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

Abstract: Methods for generating synthetic genomes, for example synthetic genomes having desired properties or viable genomes of reduced size, are disclosed. Also disclosed are synthetic genomes produced by the methods disclosed herein and synthetic cells containing the synthetic genomes disclosed herein.

Abstract: Methods for generating synthetic genomes, for example synthetic genomes having desired properties or viable genomes of reduced size, are disclosed. Also disclosed are synthetic genomes produced by the methods disclosed herein and synthetic cells containing the synthetic genomes disclosed herein.

Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

Abstract: The disclosure herein describes a message ordering system for processing and synchronizing chat messages in partitions to maintain messaging order and load balancing in a distributed system at scale. Messages are placed in partitions based on session identifiers (IDs). Messaging order is secured by a session receiver holding a lock over particular partitions. Receivers having subscription to messages in service bus are enabled to terminate on occurrence of a predetermined event, such as threshold wait time without receiving additional messages with a selected session ID from their partitions, activity level on a node, number of receivers on a node or other metrics. Session ID locks are released after a session ends, a receiver terminates or a node crashes for failure handling. New receivers are created to handle new incoming messages for additional load balancing and/or failover.

Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

Abstract: The disclosed technology is generally directed to online chat management services. In one example of the technology, organization information is accessed for tenants. The organization information includes, for each organization, configuration information for the organization. Each organization belongs to a corresponding tenant. Worker service instances are scheduled to perform fills associated with the organizations, such that sufficient fills are performed for each organization according to the configuration information for the organizations in the accessed organization information. Each fill includes: causing artifacts associated with a function to be created, such that the artifacts associated with the function are configured based on the corresponding tenant. Artifact information associated with the created artifacts is received, including at least one unique identifier associated with the created artifacts for the function.

Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

Abstract: A system generates a first data set by executing, in response to occurrence of an event, a data flow configured with a property of a first object of a first data processing system to map the first object to a second object of a second data processing system. The system acquires additional information, including additional properties of the first object and data used by the first and second data processing systems to communicate with other data processing systems, from the first and second data processing systems to map the first object to the second object. The system generates a second data set having a format compatible with the second data processing system based on the first data set and the additional information and sends the second data set to the second data processing system. The system maps other objects using reconfigured data flows.

Abstract: Disclosed herein are novel polypeptides having nuclease activity. The Mmc3 polypeptides function as Class 2 Type V effectors, and catalyze double stranded breaks in nucleic acid strands. The polypeptides are useful, for example, for gene editing systems such as CRISPR, to make site specific alterations of target nucleic acid sequences.

Abstract: A system generates a first data set by executing, in response to occurrence of an event, a data flow configured with a property of a first object of a first data processing system to map the first object to a second object of a second data processing system. The system acquires additional information, including additional properties of the first object and data used by the first and second data processing systems to communicate with other data processing systems, from the first and second data processing systems to map the first object to the second object. The system generates a second data set having a format compatible with the second data processing system based on the first data set and the additional information and sends the second data set to the second data processing system. The system maps other objects using reconfigured data flows.

Abstract: Methods for generating synthetic genomes, for example synthetic genomes having desired properties or viable genomes of reduced size, are disclosed. Also disclosed are synthetic genomes produced by the methods disclosed herein and synthetic cells containing the synthetic genomes disclosed herein.

Abstract: A system and method for providing customized content recommendations to a user based on extrapolated data is described. The system may receive an answer to a question from a user and determine a second unanswered question having a threshold relationship level with the question. The system may compute a predicted probability that the user would answer the second question correctly, calculate a first comparative skill level of the user among a cohort of similar users, and rank the first comparative skill level of the user against a second comparative skill level of the cohort to determine a skill gap of the user. Further, in some implementations, the system may generate a search query based on the skill gap of the user, and determine recommended content customized to the skill gap of the user.