Abstract: Systems, media, compositions, methods, and kits disclosed herein relate to a panel of protein biomarkers for the early detection of colon cell proliferative disorders, including colorectal cancer. The presence or levels of the proteins in a biological sample for the protein panels described herein may be used for classifier generation, and as inputs in machine learning models useful to classify subjects in a population for the detection of colon cell proliferative disorders.

Abstract: A nanoparticle conjugate includes a nanoparticle, first oligonucleotides of one or more types bound to the nanoparticle, each type of first oligonucleotide having a sequence, and targeting conjugates of one or more types, each type of targeting conjugate comprising a targeting entity and a second oligonucleotide bound to the targeting entity and having a sequence that is complementary to a sequence of a predetermined type of the first oligonucleotides, wherein the second oligonucleotide are covalently bound to a surface of the nanoparticle, a functional group on the surface of the nanoparticle, or one of the first oligonucleotide. Also provided are methods for making such nanoparticle conjugates and methods and devices for using such nanoparticle conjugates.