Abstract: An exemplary embodiment of the present disclosure provides a composition comprising two or more cytokines, and one or more of keratan sulfate, chondroitin sulfate, or hyaluronic acid. The composition can simulate a fluid from a patient. The simulated fluid has a viscosity, a storage modulus, and a loss modulus similar to that of patient-derived synovial fluid. A method for making a composition for simulating a fluid from a patient is also disclosed. The method includes creating a mixture comprising two or more cytokines, and one or more of keratan sulfate, chondroitin sulfate, or human serum albumin. The method also includes adding a low molecular weight hyaluronic acid to the mixture, adding a high molecular weight hyaluronic acid to the mixture, and incubating the mixture for a predetermined time at a temperature ranging from approximately 0° C. to approximately 10° C.

Abstract: The present disclosure provides a system for mimicking the secondary lymphoid organs where suspension cells (e.g., T cells) are expanded; methods el expanding activating, and transfecting the suspension cells in the synthetic, microenvironment, and suspension cells produced by such systems and methods.

Abstract: The various embodiments of the disclosure relate generally to processes, methods, and systems for generating functional B cells ex vivo. It is particularly useful for ex vivo generation of antigen-specific germinal-center (GC) like B cells that are capable of efficient B cell expansion, immunoglobulin (Ig) class switching/class switching recombination (CSR), expression of germinal B cell phenotypes, antibody secretion, and somatic hypermutation (SHM) and resulting affinity maturation center phenotypes.

Abstract: Provided herein are methods, compositions, and devices for occluding cavities or passageways in a patient, in particular cavities or passageways in the cardiovascular system of a patient, such as the LAA of a patient's heart. The methods, compositions, and devices can be used to percutaneously occlude the LAA, decreasing the risk of thromboembolic events associated with AF.

Abstract: Provided herein are methods, compositions, and devices for occluding cavities or passageways in a patient, in particular cavities or passageways in the cardiovascular system of a patient, such as the LAA of a patient's heart. The methods, compositions, and devices can be used to percutaneously occlude the LAA, decreasing the risk of thromboembolic events associated with AF.

Abstract: Provided herein are methods, compositions, and devices for occluding cavities or passageways in a patient, in particular cavities or passageways in the cardiovascular system of a patient, such as the LAA of a patient's heart. The methods, compositions, and devices can be used to percutaneously occlude the LAA, decreasing the risk of thromboembolic events associated with AF.

Abstract: Provided herein are methods, compositions, and devices for occluding cavities or passageways in a patient, in particular cavities or passageways in the cardiovascular system of a patient, such as the LAA of a patient's heart. The methods, compositions, and devices can be used to percutaneously occlude the LAA, decreasing the risk of thromboembolic events associated with AF.

Abstract: The invention is directed to a synthetic particle antibody comprising a bi-functional particle framework, such as for example and not limitation, a Janus micro- or nanoparticle, wherein one side of the bi-functional particle comprises targeting ligands (such as for example and not limitation, a protein, a peptide, an aptamer, and/or fragments thereof, wherein the at least one targeting ligand has the ability to specifically bind to a desired cell or tissue type in a subject's body) and the other side of the bi-functional particle comprises immune-activating ligands (such as for example and not limitation, fragments of the Fc portion of antibodies, immune-activating peptides, immune-activating aptamers, and other proteins, peptides or nucleic acids that mimic the structure and/or function of the Fc portion of antibodies).

Abstract: A multichannel multifluidic device is disclosed that can be configured to mimic the distinct microenvironments of bone marrow by providing different cell types within each channel and culturing the device under conditions to promote development of the distinct mircorenvironments.

Abstract: Provided herein are methods, compositions, and devices for occluding cavities or passageways in a patient, in particular cavities or passageways in the cardiovascular system of a patient, such as the LAA of a patient's heart. The methods, compositions, and devices can be used to percutaneously occlude the LAA, decreasing the risk of thromboembolic events associated with AF.

Abstract: Provided herein are methods, compositions, and devices for occluding cavities or passageways in a patient, in particular cavities or passageways in the cardiovascular system of a patient, such as the LAA of a patient's heart. The methods, compositions, and devices can be used to percutaneously occlude the LAA, decreasing the risk of thromboembolic events associated with AF.

Abstract: Provided herein are methods, compositions, and devices for occluding cavities or passageways in a patient, in particular cavities or passageways in the cardiovascular system of a patient, such as the LAA of a patient's heart. The methods, compositions, and devices can be used to percutaneously occlude the LAA, decreasing the risk of thromboembolic events associated with AF.

Abstract: The present disclosure provides a system for mimicking the secondary lymphoid organs where suspension cells (e.g., T cells) are expanded; methods of expanding, activating, and transfecting the suspension cells in the synthetic microenvironment, and suspension cells produced by such systems and methods.

Abstract: Disclosed herein are novel iodonium analogs having anticancer and anti-inflammatory activity.

Abstract: Provided herein are methods, compositions, and devices for occluding cavities or passageways in a patient, in particular cavities or passageways in the cardiovascular system of a patient, such as the LAA of a patient's heart. The methods, compositions, and devices can be used to percutaneously occlude the LAA, decreasing the risk of thromboembolic events associated with AF.

Abstract: Embodiments of the present disclosure can comprise a method for multiplexed analysis. The method can comprise acquiring interrogation data associated with a microstructure in a population and analyzing the microstructure based on the interrogation data. In some embodiments, the microstructure can have a different shape than at least another microstructure in the population and comprise a plurality of cells. Additionally, the acquiring the interrogation data can comprise acquiring interrogation data of microstructures in a population at a structure concentration of at least 100 microstructures/?L. Therefore, in some embodiments, acquiring the interrogation data can comprises flowing the population through a flow cytometer.

Abstract: Provided herein are methods, compositions, and devices for occluding cavities or passageways in a patient, in particular cavities or passageways in the cardiovascular system of a patient, such as the LAA of a patient's heart. The methods, compositions, and devices can be used to percutaneously occlude the LAA, decreasing the risk of thromboembolic events associated with AF.

Abstract: The present disclosure provides a system for mimicking the secondary lymphoid organs where suspension cells (e.g., T cells) are expanded; methods of expanding, activating, and transfecting the suspension cells in the synthetic microenvironment, and suspension cells produced by such systems and methods.

Abstract: The various embodiments of the disclosure relate generally to processes, methods, and systems for generating functional B cells ex vivo. It is particularly useful for ex vivo generation of antigen-specific germinal-center (GC) like B cells that are capable of efficient B cell expansion, immunoglobulin (Ig) class switching/class switching recombination (CSR), expression of germinal B cell phenotypes, antibody secretion, and somatic hypermutation (SHM) and resulting affinity maturation center phenotypes.

Abstract: Embodiments of the present disclosure can comprise a method for multiplexed analysis. The method can comprise acquiring interrogation data associated with a microstructure in a population and analyzing the microstructure based on the interrogation data. In some embodiments, the microstructure can have a different shape than at least another microstructure in the population and comprise a plurality of cells. Additionally, the acquiring the interrogation data can comprise acquiring interrogation data of microstructures in a population at a structure concentration of at least 100 microstructures/?L. Therefore, in some embodiments, acquiring the interrogation data can comprises flowing the population through a flow cytometer.