Patents by Inventor Kristen E. Opper

Kristen E. Opper has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Recombinant DNA processes using a dNTP mixture containing modified nucleotides
    Patent number: 6902914
    Abstract: Amplification mixtures, kits, amplicons, kits and processes are provided for amplifying a nucleic acid. In particular, provided are processes which utilize an amplification mixture comprising a polymerase, a deoxynucleotidetriphosphate (dNTP) mixture which contains modified dNTPs, a first primer and a second primer. Further provided are dNTP mixtures which contain modified dNTPs for at least two of the four nucleotide triphosphates, which when incorporated into a polynucelotide, impart resistance to enzymatic degradation by an exonuclease at the sites of incorporation of the modified dNTPs. Also provided are the amplicons and vectors which incorporate the modified nucleotides.
    Type: Grant
    Filed: November 15, 2001
    Date of Patent: June 7, 2005
    Assignee: Sigma-Aldrich, Co.
    Inventors: Brian Ward, Leanne M. Snyder, Chuan Li, Keming Song, Kristen E. Opper, Stephanie Uder, Ron Hernan
  • Recombinant DNA processes using a dNTP mixture containing modified nucleotides
    Publication number: 20030099956
    Abstract: Amplification mixtures, kits, amplicons, kits and processes are provided for amplifying a nucleic acid. In particular, provided are processes which utilize an amplification mixture comprising a polymerase, a deoxynucleotidetriphosphate (dNTP) mixture which contains modified dNTPs, a first primer and a second primer. Further provided are dNTP mixtures which contain modified dNTPs for at least two of the four nucleotide triphosphates, which when incorporated into a polynucelotide, impart resistance to enzymatic degradation by an exonuclease at the sites of incorporation of the modified dNTPs. Also provided are the amplicons and vectors which incorporate the modified nucleotides.
    Type: Application
    Filed: November 15, 2001
    Publication date: May 29, 2003
    Inventors: Brian Ward, Leanne M. Snyder, Chuan Li, Keming Song, Kristen E. Opper, Stephanie Uder, Ron Hernan