Patents by Inventor Kristen Kamerath Dang

Kristen Kamerath Dang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Compositions and methods for protein production
    Patent number: 11643651
    Abstract: The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.
    Type: Grant
    Filed: February 15, 2017
    Date of Patent: May 9, 2023
    Assignee: Syngenta Participations AG
    Inventors: Shib Sankar Basu, Kristen Kamerath Dang, Thomas Z. McNeill, Kasimalai Azhakanandam, Sheng Guo
  • COMPOSITIONS AND METHODS FOR PROTEIN PRODUCTION
    Publication number: 20170226504
    Abstract: The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.
    Type: Application
    Filed: February 15, 2017
    Publication date: August 10, 2017
    Applicant: Syngenta Participations AG
    Inventors: Shib Sankar BASU, Kristen Kamerath DANG, Thomas Z. MCNEILL, Kasimalai AZHAKANANDAM, Sheng Guo
  • Compositions and methods for protein production
    Patent number: 9605258
    Abstract: The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.
    Type: Grant
    Filed: January 9, 2015
    Date of Patent: March 28, 2017
    Assignee: Syngenta Participations AG
    Inventors: Shib Sankar Basu, Kristen Kamerath Dang, Kasimalai Azhakanandam, Thomas Z. McNeill, Sheng Guo
  • Compositions and methods for protein production
    Patent number: 9334494
    Abstract: The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.
    Type: Grant
    Filed: December 7, 2012
    Date of Patent: May 10, 2016
    Assignee: SYNGENTA PARTICIPATIONS AG
    Inventors: Shib Sankar Basu, Kristen Kamerath Dang, Kasimalai Azhakanandam, Thomas Z. McNeill, Sheng Guo
  • COMPOSITIONS AND METHODS FOR PROTEIN PRODUCTION
    Publication number: 20150152408
    Abstract: The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.
    Type: Application
    Filed: January 9, 2015
    Publication date: June 4, 2015
    Inventors: Shib Sankar Basu, Kristen Kamerath Dang, Kasimalai Azhakanandam, Thomas Z. McNeill, Sheng Guo